A rapid and practical prediction method for the Arizona solvent system family used in high speed countercurrent chromatography

2020 ◽  
Vol 1629 ◽  
pp. 461426
Author(s):  
Xu Wang ◽  
Chen Liu ◽  
Qing-Yun Ma ◽  
Zhen-Hua Tian ◽  
Hai-Qiang Jiang ◽  
...  
Keyword(s):  
High Speed ◽  
Prediction Method ◽  
Solvent System ◽  
Countercurrent Chromatography

scholarly journals Silver Ion-Complexation High-Speed Countercurrent Chromatography Coupled with Prep-HPLC for Separation of Sesquiterpenoids from Germacrene A Fermentation Broth

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 230
Author(s):  
Huanzhu Zhao ◽  
Xiangyun Song ◽  
Iftikhar Ali ◽  
Manzoor Hussain ◽  
Andleeb Mehmood ◽  
...  
Keyword(s):  
Metal Ion ◽  
High Speed ◽  
Fermentation Broth ◽  
Solvent System ◽  
Silver Ion ◽  
Preparative Hplc ◽  
Countercurrent Chromatography ◽  
Geometrical Isomers ◽  
Crude Sample ◽  
Counter Current

A silver ion high-speed counter-current chromatography ([Ag+]-HSCCC) was developed to separate and purify five sesquiterpenoids from germacrene A fermentation broth. The solvent system was consisted of n-hexane-methanol-silver nitrate (3 mol/L) solution (10:9.5:0.5, v/v). By employing this chromatographic protocol, five sesquiterpenoids named β-elemene (1; 54.1 mg), germacrene A (2; 28.5 mg), γ-selinene (3; 4.6 mg), β-selinene (4; 3.4 mg), and α-selinene (5; 1.3 mg) were obtained successfully from 500 mg extracted crude sample with purities of 97.1%, 95.2%, 98.2%, 96.3% and 98.5%, respectively, combined with preparative HPLC. The results reveal that the addition of metal ion in biphasic solvent system significantly improved the HSCCC separation factor of sesquiterpenoids. Meanwhile, our study also provided an alternate approach to separate the compounds with less polarity, also geometrical isomers and various natural product classes.

Separation of betacyanins from Iresine herbstii Hook. ex Lindl. leaves by high-speed countercurrent chromatography in a polar solvent system

2020 ◽  
Vol 1626 ◽  
pp. 461370 ◽  
Author(s):  
Aneta Spórna-Kucab ◽  
Natalia Wróbel ◽  
Agnieszka Kumorkiewicz-Jamro ◽  
Sławomir Wybraniec
Keyword(s):  
High Speed ◽  
Polar Solvent ◽  
Solvent System ◽  
Countercurrent Chromatography ◽  
Polar Solvent System

scholarly journals Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Gang Wang ◽  
Dan He ◽  
Fengchun Zhao ◽  
Junqiang Hu ◽  
Yin-Won Lee ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Countercurrent Chromatography ◽  
Macroporous Resin ◽  
Two Phase ◽  
Rice False Smut ◽  
Extraction And Purification ◽  
False Smut ◽  
Optimized Conditions

Abstract Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract

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