Energetic cost of physical activity in cystic fibrosis children during Pseudomonas aeruginosa pulmonary exacerbation

2005 ◽  
Vol 24 (1) ◽  
pp. 88-96 ◽  
Author(s):  
L. Béghin ◽  
F. Gottrand ◽  
L. Michaud ◽  
H. Vodougnon ◽  
N. Wizla-Derambure ◽  
...  
Keyword(s):  
Physical Activity ◽  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Energetic Cost ◽  
Pulmonary Exacerbation

Application of quantitative PCR to the diagnosis and monitoring of Pseudomonas aeruginosa colonization in 5–18-year-old cystic fibrosis patients

2011 ◽  
Vol 60 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Typhaine Billard-Pomares ◽  
Stéphanie Herwegh ◽  
Nathalie Wizla-Derambure ◽  
Dominique Turck ◽  
René Courcol ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quantitative Pcr ◽  
Sputum Sample ◽  
Pulmonary Exacerbation ◽  
Additional Tool ◽  
Bacterial Cultures ◽  
Group A ◽  
Group B ◽  
Age Range

Early detection of Pseudomonas aeruginosa and early aggressive treatment are recommended to delay chronic infection in cystic fibrosis (CF) patients. The aim of this study was to assess a quantitative PCR (q-PCR) assay for the diagnosis of early P. aeruginosa colonization in 23 young CF patients (group A, age range 7–18 years) and to survey the eradication of P. aeruginosa in 10 young CF patients (group B, age range 5–18 years) after an initial antibiotic treatment. q-PCR results for consecutive sputum samples from each patient during a period of 18 months were compared with bacterial cultures during the same period plus an additional period of 12 months, and with concomitant clinical signs of pulmonary exacerbation. The q-PCR and bacterial cultures were negative for 17 of the 23 patients in group A and six of the 10 patients in group B during the study period. However, consecutive positive q-PCR results were observed for one patient in group A and three patients in group B, while the bacterial cultures for the same sputum sample remained negative. They preceded positive P. aeruginosa bacterial cultures at 7 and 8 months for two patients in group B. These positive results were associated with a worsening of the clinical status of patients, but pulmonary exacerbation appeared non-specific for the diagnosis of early P. aeruginosa colonization since pulmonary exacerbations were observed in patients in whom q-PCR or bacterial culture remained negative. In conclusion, q-PCR may be a useful additional tool to provide information on the P. aeruginosa status of CF patients.

2-Alkyl-4-quinolone quorum sensing molecules are biomarkers for culture-independent Pseudomonas aeruginosa burden in adults with cystic fibrosis

2021 ◽  
Vol 70 (10) ◽  
Author(s):  
Nur Masirah M. Zain ◽  
Karmel Webb ◽  
Iain Stewart ◽  
Nigel Halliday ◽  
David A. Barrett ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Type Species ◽  
Bacterial Load ◽  
Pulmonary Exacerbation ◽  
Content Type ◽  
Link Type ◽  
Culture Independent ◽  
Clinical Stability

Introduction. Pseudomonas aeruginosa produces quorum sensing signalling molecules including 2-alkyl-4-quinolones (AQs), which regulate virulence factor production in the cystic fibrosis (CF) airways. Hypothesis/Gap statement. Culture can lead to condition-dependent artefacts which may limit the potential insights and applications of AQs as minimally-invasive biomarkers of bacterial load. Aim. We aimed to use culture-independent methods to explore the correlations between AQ levels and live P. aeruginosa load in adults with CF. Methodology. Seventy-five sputum samples at clinical stability and 48 paired sputum samples obtained at the beginning and end of IV antibiotics for a pulmonary exacerbation in adults with CF were processed using a viable cell separation technique followed by quantitative P. aeruginosa polymerase chain reaction (qPCR). Live P. aeruginosa qPCR load was compared with the concentrations of three AQs (HHQ, NHQ and HQNO) detected in sputum, plasma and urine. Results. At clinical stability and the beginning of IV antibiotics for pulmonary exacerbation, HHQ, NHQ and HQNO measured in sputum, plasma and urine were consistently positively correlated with live P. aeruginosa qPCR load in sputum, compared to culture. Following systemic antibiotics live P. aeruginosa qPCR load decreased significantly (P<0.001) and was correlated with a reduction in plasma NHQ (plasma: r=0.463, P=0.003). Conclusion. In adults with CF, AQ concentrations correlated more strongly with live P. aeruginosa bacterial load measured by qPCR compared to traditional culture. Prospective studies are required to assess the potential of systemic AQs as biomarkers of P. aeruginosa bacterial burden.

scholarly journals Multidrug-Resistant Pseudomonas aeruginosa Infection in a Child with Cystic Fibrosis

2016 ◽  
Vol 60 (10) ◽  
pp. 5627-5630 ◽  
Author(s):  
Jocelyn Y. Ang ◽  
Nahed Abdel-Haq ◽  
Frank Zhu ◽  
Abrar K. Thabit ◽  
David P. Nicolau ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Cystic Fibrosis Patient ◽  
Multidrug Resistant ◽  
Pulmonary Exacerbation ◽  
Content Type ◽  
Pseudomonas Aeruginosa Infection

ABSTRACTWe describe a pediatric cystic fibrosis patient who developed a pulmonary exacerbation due to two multidrug-resistant (MDR)Pseudomonas aeruginosaisolates. In addition to these MDR organisms, the case was further complicated by β-lactam allergy. Despite the MDR phenotype, both isolates were susceptible to an antimicrobial combination.

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