Novel carrier-free nanoparticles composed of 7-ethyl-10-hydroxycamptothecin and chlorin e6: Self-assembly mechanism investigation and in vitro/in vivo evaluation

2020 ◽  
Vol 188 ◽  
pp. 110722 ◽  
Author(s):  
Yanna Zhao ◽  
Yuping Zhao ◽  
Qisan Ma ◽  
Huaizhen Zhang ◽  
Yinglin Liu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Chlorin E6 ◽  
In Vivo Evaluation ◽  
Assembly Mechanism ◽  
Carrier Free

scholarly journals Carrier-free, self-assembled pure drug nanorods composed of 10-hydroxycamptothecin and chlorin e6 for combinatorial chemo-photodynamic antitumor therapy in vivo

Nanoscale ◽  
2017 ◽  
Vol 9 (38) ◽  
pp. 14347-14356 ◽  
Author(s):  
Yan Wen ◽  
Wei Zhang ◽  
Ningqiang Gong ◽  
Yi-Feng Wang ◽  
Hong-Bo Guo ◽  
...  
Keyword(s):  
Combination Therapy ◽  
Self Assembly ◽  
Chlorin E6 ◽  
Antitumor Therapy ◽  
Carrier Free ◽  
Pure Drug ◽  
Self Assembled

Carrier-free nanodrug HCPT/Ce6 NRs were prepared through a simple self-assembly approach for chemo-photodynamic combination therapy of tumors in vivo.

Acidity-responsive shell-sheddable camptothecin-based nanofibers for carrier-free cancer drug delivery

Nanoscale ◽  
2019 ◽  
Vol 11 (34) ◽  
pp. 15907-15916 ◽  
Author(s):  
Zhuha Zhou ◽  
Ying Piao ◽  
Lingqiao Hao ◽  
Guanyu Wang ◽  
Zhuxian Zhou ◽  
...  
Keyword(s):  
Self Assembly ◽  
Surface Coating ◽  
Tumor Tissue ◽  
The Self ◽  
Cancer Drug ◽  
Ph Responsive ◽  
Carrier Free ◽  
Cancer Drug Delivery

pH-responsive nanofibers are obtained by the self-assembly of the camptothecin prodrug and surface-coating, which can efficiently enter cancer cells in vitro and penetrate deep into tumor tissue in vivo.

scholarly journals Turing miRNA into infinite coordination supermolecule: a general and enabling nanoengineering strategy for resurrecting nuclear acid therapeutics

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Liya Li ◽  
Wangxiao He ◽  
Weiming You ◽  
Jin Yan ◽  
Wenjia Liu
Keyword(s):  
Self Assembly ◽  
Wnt Signaling Pathway ◽  
Pdx Model ◽  
Carrier Free ◽  
Rapid Clearance ◽  
Targeting Ability ◽  
Time Injection ◽  
General Method

Abstract Background Clinical translation of therapeutic nuclear acid, particularly those targeting tumor progression, has been hampered by the intrinsic weaknesses of nuclear acid therapeutic including poor systemic stability, rapid clearance, low membrane permeability and lack of targeting ability. Small nuclear acid engineered into carrier-free nanodrugs with structural stability and disease targeting may be viable to overcome pharmaceutical obstacles of nuclear acid. Methods A general method through a mild and simple chemistry was established to convert therapeutic miRNA into an infinite Auric-sulfhydryl coordination supramolecular miRNA termed IacsRNA with near-spherical nanostructure, high colloid as well as anti-hydrolysis stability and low macrophage uptakes. Results IacsRNA presented the increased half-life period in circulation and accumulation at tumor sites in comparison to normal miRNA. Moreover, Iacs-miR-30c showed no toxicity of viscera and sanguis system in the 5-time injection dosage of the treatment. More importantly, Iacs-miR-30c potently suppressed the Wnt signaling pathway in vitro and in vivo, and effectively sensitized both potency of 5-Fu in PDX model of colon cancer and Anti-PD1 in B16F10 homograft model of melanoma. Conclusion Collectively, this work amply confirmed the design of IacsRNA as a general and viable strategy of nano-pharmaceutic to concert flimsy therapeutic miRNA into potential drugs. Considering from a broader perspective, the miRNA-initiated infinite coordination self-assembly strategy has distinct advantages in resurrecting nuclear acid therapeutics, probably bringing new inspiration to RNA-derived therapeutics of a great variety of human diseases including cancer. Graphical Abstract

Carrier free rapamycin loaded drug eluting stent: In vitro and in vivo evaluation

2013 ◽  
Vol 168 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Wahid Khan ◽  
Shady Farah ◽  
Abraham Nyska ◽  
Abraham J. Domb
Keyword(s):  
Drug Eluting Stent ◽  
In Vivo Evaluation ◽  
Carrier Free ◽  
Drug Eluting

Self-assembled 5-fluorouracil-cinnamaldehyde nanodrugs for greatly improved chemotherapy in vivo

2021 ◽  
pp. 088532822198953
Author(s):  
Qin Fang ◽  
Xiaoxiao Xu ◽  
Longshun Yang ◽  
Yanbing Xue ◽  
Xu Cheng ◽  
...  
Keyword(s):  
Side Effects ◽  
Self Assembly ◽  
Metabolic Effects ◽  
Tumor Growth Inhibition ◽  
Ester Bond ◽  
Carrier Free ◽  
Drug Activities ◽  
Dual Drug

The preferred cancer treatment is to achieve a high therapeutic effect as well as reduce side effects. In this study, we developed carrier-free nano drugs based on 5-fluorouracil (5FU) and cinnamaldehyde (CA) to meet the above goals. Two model drugs were spliced by acetal linkage and ester bond, which could self-assemble into nano drug particles (5FU–CA NPs) with a size of ∼170 nm. In vitro cell experiments showed 5FU–CA NPs were efficiently internalized by HepG2 cells. They then quickly exerted dual drug activities by the cleavage of acetal and ester bond, resulting in enhanced cell-killing efficacy and apoptosis. Synergistic mechanisms were achieved via the anti-metabolic effects mediated by 5FU–COOH and the oxidative damage induced by CA. In vivo anti-tumor evaluation further indicated that 5FU–CA NPs had higher tumor growth inhibition than 5FU–COOH/CA mixture (5FU–COOH + CA) and exhibited lower systemic toxicity under the same reducing dose of each drug. Overall, this is a successful synergistic anti-tumor attempt through rational self-assembly of drugs with different mechanisms and it can be extrapolated to other agents.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

Programmable in vitro Co-Encapsidation of Guest Proteins Inside Protein Nanocages

2018 ◽  
Author(s):  
Noor H. Dashti ◽  
Rufika S. Abidin ◽  
Frank Sainsbury
Keyword(s):  
Self Assembly ◽  
Mammalian Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Cell Engineering ◽  
Particle Analysis ◽  
Protein Cages

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both <i>in vitro</i> and <i>in vivo</i> cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for <i>in vivo</i> self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by <i>in vitro</i> self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

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