Bipartite molecular approach for species delimitation and resolving cryptic speciation of Exobasidium vexans within the Exobasidium genus

2021 ◽  
Vol 92 ◽  
pp. 107496
Author(s):  
Chayanika Chaliha ◽  
V. Chandra Kaladhar ◽  
Robin Doley ◽  
Praveen Kumar Verma ◽  
Aditya Kumar ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Cryptic Speciation ◽  
Molecular Approach

Species delimitation without prior knowledge: DISSECT reveals extensive cryptic speciation in the Silene aegyptiaca complex (Caryophyllaceae)

2016 ◽  
Vol 102 ◽  
pp. 1-8 ◽  
Author(s):  
Zeynep Toprak ◽  
Bernard E. Pfeil ◽  
Graham Jones ◽  
Thomas Marcussen ◽  
Alaattin Selçuk Ertekin ◽  
...  
Keyword(s):  
Prior Knowledge ◽  
Species Delimitation ◽  
Cryptic Speciation

scholarly journals Testing species hypotheses for Fridericia magna, an enchytraeid worm (Annelida: Clitellata) with great mitochondrial variation

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Svante Martinsson ◽  
Mårten Klinth ◽  
Christer Erséus
Keyword(s):  
Species Delimitation ◽  
Single Species ◽  
The Other ◽  
Cryptic Speciation ◽  
Incipient Speciation ◽  
Nuclear Markers ◽  
Separate Species ◽  
Mitochondrial Markers ◽  
Morphological Differences ◽  
Barcode Gap

Abstract Background Deep mitochondrial divergences were observed in Scandinavian populations of the terrestrial to semi-aquatic annelid Fridericia magna (Clitellata: Enchytraeidae). This raised the need for testing whether the taxon is a single species or a complex of cryptic species. Results A total of 62 specimens from 38 localities were included in the study, 44 of which were used for species delimitation. First, the 44 specimens were divided into clusters using ABGD (Automatic Barcode Gap Discovery) on two datasets, consisting of sequences of the mitochondrial markers COI and 16S. For each dataset, the worms were divided into six not completely congruent clusters. When they were combined, a maximum of seven clusters, or species hypotheses, were obtained, and the seven clusters were used as input in downstream analyses. We tested these hypotheses by constructing haplowebs for two nuclear markers, H3 and ITS, and in both haplowebs the specimens appeared as a single species. Multi-locus species delimitation analyses performed with the Bayesian BPP program also mainly supported a single species. Furthermore, no apparent morphological differences were found between the clusters. Two of the clusters were partially separated from each other and the other clusters, but not strongly enough to consider them as separate species. All 62 specimens were used to visualise the Scandinavian distribution, of the species, and to compare with published COI data from other Fridericia species. Conclusion We show that the morphospecies Fridericia magna is a single species, harbouring several distinct mitochondrial clusters. There is partial genetic separation between some of them, which may be interpreted as incipient speciation. The study shows the importance of rigorous species delimitation using several independent markers when deep mitochondrial divergences might give the false impression of cryptic speciation.

Out of the deep: Cryptic speciation in a Neotropical gecko (Squamata, Phyllodactylidae) revealed by species delimitation methods

2014 ◽  
Vol 80 ◽  
pp. 113-124 ◽  
Author(s):  
Fabricius M.C.B. Domingos ◽  
Renan J. Bosque ◽  
José Cassimiro ◽  
Guarino R. Colli ◽  
Miguel T. Rodrigues ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Cryptic Speciation

scholarly journals Cryptic speciation in populations of the genus Aphyocharax (Characiformes: Characidae) from eastern Amazon coastal river drainages and surroundings revealed by single locus species delimitation methods

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Pâmella S. Brito ◽  
Erick C. Guimarães ◽  
Karen L. A. Guimarães ◽  
Luís R. R. Rodrigues ◽  
Marcelo R. Anjos ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Fish Species ◽  
Species Delimitation ◽  
Morphological Differentiation ◽  
Single Locus ◽  
Cryptic Speciation ◽  
Coastal River ◽  
River Drainages ◽  
New Fish Species

Abstract Recent studies in eastern Amazon coastal drainages and their surroundings have revealed new fish species that sometimes exhibit little morphological differentiation (cryptic species). Thus, we used a DNA-based species delimitation approach to test if populations showing the morphotype and typical character states of the Aphyocharax avary holotype correspond either to A. avary or A. brevicaudatus, two known species from the region, or if they form independent lineages, indicating cryptic speciation. WP and GMYC analyses recovered five lineages (species) in the ingroup, while a bPTP analysis delimited three lineages. ABGD analyses produced two possible results: one corroborating the WP and GMYC methods and another corroborating the bPTP method. All methods indicate undescribed cryptic species in the region and show variation from at least 1 to 4 species in the ingroup, depending on the approach, corroborating previous studies, and revealing this region as a possible hotspot for discovering undescribed fish species.

scholarly journals Phylogeography, species delimitation and population structure of a Western Australian short-range endemic mite harvestman (Arachnida: Opiliones: Pettalidae: Karripurcellia)

2018 ◽  
Vol 2 (1) ◽  
pp. 81-87
Author(s):  
Martin Schwentner ◽  
Gonzalo Giribet
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Species Delimitation ◽  
National Park ◽  
Short Range ◽  
Cryptic Speciation ◽  
Mtdna Sequences ◽  
Single Population ◽  
Eucalypt Forests ◽  
Western Australian

The mite harvestmen of the genusKarripurcelliaGiribet, 2003 are endemic to the tall, wet eucalypt forests of south-western Western Australia, a region known as a hotspot for biodiversity. Currently, there are two accepted species,K.peckorumGiribet, 2003 andK.sierwaldaeGiribet, 2003, both with type localities within the Warren National Park. We obtained 65COImtDNA sequences from across the entire distributional range of the genus. These sequences, falling into two to three geographically separate groups, probably correspond to two species. Morphologically, all of the studied specimens correspond toK.peckorum, suggesting cryptic speciation within that species. A few common haplotypes occur in more than one population, but most haplotypes are confined to a single population. As a result, populations are genetically differentiated and gene flow after initial colonization appears to be very limited or completely lacking. Our study provides another example of short-range endemism in an invertebrate from the south-western mesic biome.

scholarly journals Opening the Pandora Box: DNA-barcoding evidence limitations of morphology to identify Spanish mosquitoes

2018 ◽  
Author(s):  
Delgado-Serra Sofía ◽  
Viader Miriam ◽  
Ruiz-Arrondo Ignacio ◽  
Miranda Miguel Ángel ◽  
Barceló Carlos ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Species Delimitation ◽  
Morphological Identification ◽  
Cryptic Speciation ◽  
Central Question ◽  
Tropical Regions ◽  
Invasive Mosquito ◽  
Anopheles Algeriensis ◽  
Typological Approach ◽  
La Rioja

AbstractCryptic speciation is frequent in the medically important mosquitoes. While most findings have been reported in tropical regions, it is an unexplored topic in countries where mosquito-borne diseases are not endemic, like Spain. The occurrence of recent outbreaks in Europe has increased the awareness on the native and invasive mosquito fauna present in the continent. Therefore, the central question of this study is whether the typological approach is sufficient to identify Spanish mosquitoes. To address this problem, we confronted the results of the morphological identification of 62 adult specimens collected from four different regions of Spain (La Rioja, Navarra, Castellón and the Island of Majorca) with the results obtained through DNA-barcoding. We conducted a comprehensive phylogenetic analysis of the COI gene region and compared this with the results of four species delimitation algorithms (ABGD initial partition, ABGD P=0.46%, bPTP and TCS). We report strong evidence for cryptic speciation in Anopheles algeriensis and Aedes vexans and reproductive isolation of the rock pool mosquito Aedes mariae. In addition, we report that the character present in the wings is not efficient to distinguish species Culiseta annulata from Culiseta subochrea, which distribution in the country may be different than previously described.

When rare species become endangered: cryptic speciation in myrmecophilous hoverflies

2002 ◽  
Vol 76 (2) ◽  
pp. 315-315
Author(s):  
KARSTEN SCHONROGGE ◽  
BOYD BARR ◽  
JUDITH C. WARDLAW ◽  
EMMA NAPPER ◽  
MICHAEL G. GARDNER ◽  
...  
Keyword(s):  
Rare Species ◽  
Cryptic Speciation

A molecular approach for the discrimination of medicinal Astragali radix used in TCM

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
G Heubl ◽  
K Woelkart ◽  
EM Wenzig ◽  
B Heydel ◽  
R Bauer
Keyword(s):  
Molecular Approach ◽  
Astragali Radix

Notes on the molecular taxonomy of the Proclossiana eunomia complex (Lepidoptera, Nymphalidae: Argynnini): analysis of DNA barcodes

2018 ◽  
Vol 8 (1) ◽  
pp. 222-232 ◽  
Author(s):  
R. V. Yakovlev ◽  
N. A. Shapoval ◽  
G. N. Kuftina ◽  
A. V. Kulak ◽  
S. V. Kovalev
Keyword(s):  
Body Size ◽  
Molecular Taxonomy ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Molecular Approach ◽  
Dna Barcodes ◽  
Morphological Differences ◽  
Proclossiana Eunomia

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

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