Notes on the molecular taxonomy of the Proclossiana eunomia complex (Lepidoptera, Nymphalidae: Argynnini): analysis of DNA barcodes

2018 ◽  
Vol 8 (1) ◽  
pp. 222-232 ◽  
Author(s):  
R. V. Yakovlev ◽  
N. A. Shapoval ◽  
G. N. Kuftina ◽  
A. V. Kulak ◽  
S. V. Kovalev
Keyword(s):  
Body Size ◽  
Molecular Taxonomy ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Molecular Approach ◽  
Dna Barcodes ◽  
Morphological Differences ◽  
Proclossiana Eunomia

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

scholarly journals Combination of nuclear and mitochondrial markers as a useful tool to identify Ctenophthalmus species and subspecies (Siphonaptera: Ctenophthalmidae)

2021 ◽  
Author(s):  
Antonio Zurita ◽  
Cristina Cutillas
Keyword(s):  
Taxonomic Status ◽  
Molecular Study ◽  
Molecular Data ◽  
Point Of View ◽  
Morphological Data ◽  
Molecular Approach ◽  
Nuclear Marker ◽  
Mitochondrial Markers ◽  
Morphological Criteria ◽  
Mtdna Markers

AbstractCtenophthalmus is considered the largest genus within the Order Siphonaptera. From a morphological point of view, only males of this genus can be identified at species and subspecies levels using morphological keys, whereas there are no morphological criteria in order to classify females at these taxonomical levels. Furthermore, the amount of available molecular and phylogenetic data for this genus is quite scarce so far. The main objective of this work was to assess the utility of the combination of nuclear and mitochondrial markers with respect to their ability to differentiate among different subspecies within the Ctenophthalmus genus. With this purpose, we carried out a comparative morphological and molecular study of three different subspecies (Ctenophthalmus baeticus arvernus, Ctenophthalmus nobilis dobyi, and Ctenophthalmus andorrensis catalaniensis) in order to clarify and discuss its taxonomic status. In addition, our study complemented the molecular data previously provided for Ctenophthalmus baeticus boisseauorum and Ctenophthalmus apertus allani subspecies. We sequenced five different molecular markers: EF1-α, ITS1, ITS2, cox1, and cytb. Our results confirmed that morphological data by themselves are not able to discriminate among Ctenophthalmus female taxa; however, the combination of the nuclear marker EF1-α together with mtDNA markers cytb and cox1 constituted a useful taxonomical and phylogenetic tool to solve this issue. Based on these results, we consider that the use of this molecular approach should be gradually used within Ctenophthalmus genus in order to complement its classical taxonomy and clarifying the complex taxonomy of other congeneric species of fleas.

A new species of Carpenter Moths (Lepidoptera, Cossidae) from Tarbagatai (NE Kazakhstan) and Altai (SW Siberia, Russia) Mountains

Zootaxa ◽  
2020 ◽  
Vol 4896 (1) ◽  
pp. 85-95
Author(s):  
ROMAN V. YAKOVLEV ◽  
NAZAR A. SHAPOVAL ◽  
VADIM V. IVONIN ◽  
SVYATOSLAV A. KNYAZEV ◽  
GALINA N. KUFTINA ◽  
...  
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Altai Mountains ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

We described a new cossid species, Dyspessa ulgen sp. nov. from the Tarbagatai and Altai Mountains and compared it to other taxa of Dyspessa reported from the region (D. tristis, D. saldaitisi, D. saissanica), as well as to morphologically similar D. ulula. The new species is most closely related to D. ulula but differs from the latter in the characteristics of the male genitalia, wing pattern, and molecular data (a 658 bp fragment of the mitochondrial COI gene). 

Regarding the taxonomic status of Ophyra Robineau-Desvoidy (Diptera: Muscidae): A molecular approach

Zootaxa ◽  
2004 ◽  
Vol 712 (1) ◽  
pp. 1 ◽  
Author(s):  
GUILHERME SCHNELL E. SCHUEHLI ◽  
CLAUDIO JOSÉ BARROS DE CARVALHO ◽  
BRIAN M. WIEGMANN
Keyword(s):  
Elongation Factor ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Systematic Position ◽  
Mitochondrial Genes ◽  
Phylogenetic Study ◽  
Molecular Approach ◽  
The Family ◽  
The Subject ◽  
Elongation Factor 1

The muscid genus Ophyra has long been the subject of debate over its placement within the family. However, a phylogenetic study has never been conducted that would clarify its systematic position. In the present paper, phylogenetic relationships are examined between Ophyra albuquerquei and related muscid genera. The mitochondrial genes Cytochrome Oxidase I and II and tRNA-Leu were used combined with the nuclear genes CAD and Elongation Factor 1 to compose a matrix with 2989 characters (716 parsimonyinformative). These characters were analyzed under parsimony resulting in a single most parsimonious tree. Contrary to some recent classifications, our molecular data suggest the placement of Ophyra albuquerquei within the Muscinae in a separate position from the azeliine genus Hydrotaea.

scholarly journals New insight into the taxonomic resolution of the genus Bythotrephes Leydig (Crustacea: Cladocera) based on molecular data from Central Europe

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maciej Karpowicz ◽  
Magdalena Świsłocka ◽  
Joanna Moroz ◽  
Łukasz Sługocki
Keyword(s):  
Genetic Analysis ◽  
Central Europe ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Taxonomic Resolution ◽  
Monotypic Genus ◽  
Mtdna Coi ◽  
Xix Century ◽  
Insight Into

AbstractThe taxonomic status of the genus Bythotrephes Leydig (Crustacea: Cladocera) has been debated since the second half of the XIX century. The most widespread view of recent decades has been that Bythotrephes is a monotypic genus, which was support by preliminary molecular data. However, the recent detailed morphological revision of this genus clearly distinguishes at least seven species. Therefore, we performed a multi-lake survey in Central Europe to give new insight into the taxonomic status of Bythotrephes by combining genetic analysis with traditional morphology-based taxonomy. Based on the morphology we identified two species in Central Europe, B. brevimanus and B. lilljeborgi, as well as hybrid forms. For the genetic analysis, we used newly obtained 113 sequences of mtDNA COI gene of the 535-bp length Bythotrephes from Central Europe and sequences downloaded from GenBank. There were no significant differences between all analyzed sequences, which supports the hypothesis that Bythotrephes is a monotypic genus, with only one highly polymorphic species. On the other hand, the results of our work could point out that the COI gene is insufficient to evaluate the taxonomic status of Bythotrephes. Nonetheless, we have identified 29 new haplotypes of mtDNA COI, and one which was the same as the haplotype found in North America and Finland. Furthermore, this haplotype was the source variant from which most other haplotypes were derived.

scholarly journals Eidophasia assmanni sp. nov., the first alpine representative of the genus, detected in the Russian Altai Mountains (Lepidoptera, Plutellidae)

ZooKeys ◽  
2020 ◽  
Vol 959 ◽  
pp. 99-111
Author(s):  
Peter Huemer ◽  
Jae-Cheon Sohn
Keyword(s):  
New Species ◽  
Endemic Species ◽  
Molecular Data ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Altai Mountains ◽  
Male Adult ◽  
Alpine Zone ◽  
A New Species ◽  
Russian Altai

Eidophasia assmannisp. nov., a new species of Plutellidae from the alpine zone of Russian Altai Mountains, is described from diagnostic morphology and DNA barcodes. Male adult and genitalia are illustrated, whereas the female sex remains unknown. The species inhabits alpine scree with patchy herbaceous plants and is considered as possible endemic species of the Altai Mountains. An updated checklist of the 13 global Eidophasia Stephens, 1842 species is provided. The likely polyphyly of the genus is discussed from molecular data of the barcode region of the mt COI gene.

Naming species with no morphological indicators: species status of Galeolaria caespitosa (Annelida:Serpulidae) inferred from nuclear and mitochondrial gene sequences and morphology

2009 ◽  
Vol 23 (3) ◽  
pp. 205 ◽  
Author(s):  
Magdalena N. Halt ◽  
Elena K. Kupriyanova ◽  
Steven J. B. Cooper ◽  
Greg W. Rouse
Keyword(s):  
Cytochrome B ◽  
Mitochondrial Gene ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Pairwise Distance ◽  
South Wales ◽  
Western Group ◽  
Mitochondrial Cytochrome B ◽  
Group Variation ◽  
Morphological Differences

Galeolaria caespitosa Lamarck, 1818 is an endemic, gregarious serpulid annelid, ubiquitous along the intertidal zone of southern Australia, occurring from Queensland to Western Australia. We sampled specimens across this range and utilised morphological features and sequences of mitochondrial (cytochrome-b) and nuclear (ITS2) markers to assess the taxonomic status of this morphospecies. No taxonomically significant morphological differences were observed across the range of G. caespitosa. However, the molecular data revealed the existence of the following two well supported clades that were also geographically concordant for the two markers: an eastern clade, consisting of New South Wales and southern Queensland samples, and a south-western group that encompassed samples from the rest of its range. The minimum pairwise distance between members of the two groups was more than 24% for cytochrome-b, with a maximum of 1% within-group variation. In addition, analysis of molecular variation showed a high proportion (97%) of the total variation distributed among the two groups, indicative of long-term isolation of the two clades. These results suggest that G. caespitosa comprises at least two cryptic species. Here, we discuss the merits of naming new Galeolaria species, given there were no consistent morphological differences detectable and the absence of details on the type locality for G. caespitosa. We conclude that a new species of Galeolaria is warranted and describe it here as G. gemineoa.

A new species of Anurophorus (Collembola: Isotomidae) from South Korea, with notes on its molecular data

Zootaxa ◽  
2021 ◽  
Vol 4985 (3) ◽  
Author(s):  
GYU-DONG CHANG ◽  
MIKHAIL POTAPOV ◽  
KYUNG-HWA PARK
Keyword(s):  
New Species ◽  
South Korea ◽  
Dna Sequences ◽  
Ventral Side ◽  
Molecular Data ◽  
Coi Gene ◽  
Species Discrimination ◽  
Dna Barcodes ◽  
Abies Koreana ◽  
A New Species

A new species, Anurophorus hallaensis sp. nov. was collected from the withered leaves of Korean fir (Abies koreana Wilson), which is distributed at higher than 1,300 m in altitude on Mt. Hallasan (Jeju Island, South Korea). The morphology and two partial gene regions of this species are described herein. Anurophorus hallaensis sp. nov. showed the same formulas of macrochaetae and sensilla on thoracic segment II to abdominal segment IV as those exhibited by A. laricis Nicolet and A. palearcticus Potapov; however, this new species can be distinguished from them by the number of knobbed hairs on ventral side of legs. Partial DNA sequences of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene and ribosomal DNA (28S rDNA) were used as DNA barcodes to distinguish between A. hallaensis sp. nov. and closely related congeners. The results of the present study indicate that the COI and rDNA are useful for species discrimination within the genus Anurophorus. An identification key to the Korean species of Anurophorus is provided.

scholarly journals Molecular characteristics and taxonomic status of morphologically similar barnacles (Amphibalanus) assessed using the cytochrome c oxidase 1 gene

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Sri Riani ◽  
ROMANUS EDY PRABOWO ◽  
Agus Nuryanto
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Field Trips ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Severe Problem ◽  
Molecular Barcoding ◽  
Level Identification

Abstract. Riani S, Prabowo RE, Nuryanto A. 2021. Molecular characteristics and taxonomic status of morphologically similar barnacles (Amphibalanus) assessed using the cytochrome c oxidase 1 gene. Biodiversitas 22: 1456-1466. Amphibalanus variegatus and A. reticulatus have similar external morphology. Morphological similarities can be a severe problem for direct species-level identification. The problem can be overcome through anatomy-based identification and validated through molecular barcoding. Molecular characterization using the cytochrome c oxidase 1 (COI) gene provides a useful tool for precise species identification. This study attempted to assess the molecular characteristics of morphologically similar barnacle (Amphibalanus) specimens collected at five localities in Indonesia to validate their taxonomic status. Forty-five barnacle specimens were collected during the field trips in Lampung, Jakarta, Semarang, Bali, and Lombok. The COI gene was amplified using LCO1490 and HCO2198 primers. The gene was sequenced using bidirectional sequencing at 1st base Asia. The specimens' taxonomic status was determined based on sequence identity, genetic distance, monophyly, nucleotide compositions, and nucleotides in a particular position. Shell shapes-based identification placed barnacle specimens into A. reticulatus. However, anatomical-based identification placed barnacle samples into two different anatomic groups, which was further validated by molecular data that two anatomic groups of Amphibalanus samples have significant differences in their COI gene. Based on the molecular characteristics, 43 samples were identified as A. reticulatus, while the two remaining samples were identified as A. variegatus.

scholarly journals The taxonomic status of Cossus cossus afghanistanus (Lepidoptera, Cossidae) from Afghanistan: insights from molecular and morphological data

2017 ◽  
Vol 7 (3) ◽  
pp. 134-138 ◽  
Author(s):  
N. A. Shapoval ◽  
R. V. Yakovlev ◽  
G. N. Kuftina
Keyword(s):  
Phylogenetic Analysis ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Morphological Data ◽  
Separate Species ◽  
Morphological Differences

<p>In our study we use a 658 bp fragment of the <em>COI</em> gene to analyze a taxon from Afghanistan usually treated in literature as <em>Cossus cossus afghanistanus </em>(Daniel, 1953)<em>.</em> The previous conclusions on taxonomy and nomenclature were not supported by molecular data therefore the question of identity of this taxon has remained unverified. Phylogenetic analysis revealed <em>C. c. afghanistanus </em>to be strongly differentiated from nominotypical <em>Cossus cossus</em> (Linnaeus, 1758) (p-distance: 6.7% ± 1.5%). <em>Cossus c. afghanistanus </em>forms a distinct well-supported clade in ML and BI trees. This fact, together with prominent morphological differences (wing color and genitalia structure) shows that <em>C. c. afghanistanus </em>represent a separate species rather than a subspecies of <em>Cossus cossus</em>.</p>

Serpinema cayennensis n. sp. (Nematoda: Camallanidae), a parasite of the freshwater turtle Rhinoclemmys punctularia Daudin (Reptilia: Testudines: Geoemydidae) from French Guiana: morphology and phylogenetic relationships with other turtle-parasitising camallanids

Zootaxa ◽  
2019 ◽  
Vol 4679 (1) ◽  
pp. 181-193
Author(s):  
FLORENCE HARNOSTER ◽  
ROMAN SVITIN ◽  
LOUIS DU PREEZ
Keyword(s):  
French Guiana ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Molecular Data ◽  
Coi Gene ◽  
Freshwater Turtle ◽  
Freshwater Turtles ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit 1 ◽  
A New Species

The genus Serpinema was erected from the genus Camallanus to include species parasitising freshwater turtles. Following this, the taxonomic status of Serpinema was challenged by different authors considering it as a subgenus or junior synonym of Camallanus. Several specimens of these nematodes were retrieved from the spot-legged wood turtle Rhinoclemmys punctularia from Cayenne, French Guiana. These specimens clearly differed from previously reported Serpinema and Camallanus species parasitising turtles by the number of caudal papillae, shape of spicules and development of vulvar lips. Based on morphological characters the nematodes were assigned to the genus Serpinema and described as a new species S. cayennensis n. sp. The description followed by the molecular data analyses based on 28S, 18S rDNA and cytochrome oxidase subunit 1 (COI) gene sequences. 

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