Multi-Model CNN Fusion for Sperm Morphology Analysis

Sperm morphology analysis is a fundamental component of semen analysis, but its real significance has been clouded by the plethora of techniques used for its evaluation. Most involve different fixation and staining procedures that induce artefacts. Herein we describe Trumorph (Proiser R+D, Paterna, Spain), a new method for sperm morphology analysis based on examination of wet preparations of spermatozoa immobilised, after a short 60°C shock, in narrow chambers and examined by negative phase contrast microscopy. A range of morphological forms was observed, similar to those found using conventional fixed and stained preparations, but other forms were also found, distinguishable only by the optics used. The ease of preparation makes the Trumorph a robust method applicable for the analysis of living unmodified spermatozoa in a range of situations. Subsequent studies on well-characterised samples are required to describe the morphology of spermatozoa with fertilising potential.

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Interpretation of sperm morphology analysis in gynecological practice for infertility

Expert Review of Obstetrics & Gynecology ◽

10.1586/17474108.1.1.7 ◽

2006 ◽

Vol 1 (1) ◽

pp. 7-9

Author(s):

Nicolás Garrido ◽

Marcos Meseguer ◽

Antonio Pellicer

Keyword(s):

Sperm Morphology ◽

Morphology Analysis

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Have We Conquered Sperm Morphology Analysis in Different Mammalian Species as Analysed by CASMA?

XIIIth International Symposium on Spermatology ◽

10.1007/978-3-030-66292-9_38 ◽

2021 ◽

pp. 287-301

Author(s):

Gerhard van der Horst ◽

Stefan S. du Plessis ◽

Liana Maree

Keyword(s):

Sperm Morphology ◽

Mammalian Species ◽

Morphology Analysis

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Assessment of stallion semen morphology using two different staining methods, microscopic techniques, and sample sizes

Journal of Veterinary Research ◽

10.1515/jvetres-2016-0014 ◽

2016 ◽

Vol 60 (1) ◽

pp. 99-104 ◽

Cited By ~ 7

Author(s):

Katarzyna Łącka ◽

Stanisław Kondracki ◽

Maria Iwanina ◽

Anna Wysokińska

Keyword(s):

Sperm Morphology ◽

Staining Method ◽

Microscopic Analysis ◽

Sample Sizes ◽

Morphology Analysis ◽

Morphological Defects ◽

Staining Methods ◽

Microscopic Techniques ◽

Semen Morphology

Abstract Introduction: The aim of this study was to propose the optimal methodology for stallion semen morphology analysis while taking into consideration the staining method, the microscopic techniques, and the workload generated by a number of samples. Material and Methods: Ejaculates from eight pure-bred Arabian horses were tested microscopically for the incidence of morphological defects in the spermatozoa. Two different staining methods (eosin-nigrosin and eosin-gentian dye), two different techniques of microscopic analysis (1000× and 400× magnifications), and two sample sizes (200 and 500 spermatozoa) were used. Results: Well-formed spermatozoa and those with major and minor defects according to Blom’s classification were identified. The applied staining methods gave similar results and could be used in stallion sperm morphology analysis. However, the eosin-nigrosin method was more recommendable, because it allowed to limit the number of visible artefacts without hindering the identification of protoplasm drops and enables the differentiation of living and dead spermatozoa. Conclusion: The applied microscopic techniques proved to be equally efficacious. Therefore, it is practically possible to opt for the simpler and faster 400x technique of analysing sperm morphology to examine stallion semen. We also found that the number of spermatozoa clearly affects the results of sperm morphology evaluation. Reducing the number of spermatozoa from 500 to 200 causes a decrease in the percentage of spermatozoa identified as normal and an increase in the percentage of spermatozoa determined as morphologically defective.

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