Multiplex PCR assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae with an internal amplification control

2014 ◽  
Vol 79 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Shuang Wei ◽  
Hui Zhao ◽  
Yuyin Xian ◽  
Malik A. Hussain ◽  
Xiyang Wu
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Vibrio Alginolyticus ◽  
Internal Amplification Control ◽  
Pcr Assays

A Collagenase-Targeted Multiplex PCR Assay for Identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus

2005 ◽  
Vol 68 (1) ◽  
pp. 150-153 ◽  
Author(s):  
ANGELA DI PINTO ◽  
GIUSEPPINA CICCARESE ◽  
GIUSEPPINA TANTILLO ◽  
DOMENICO CATALANO ◽  
VITO TONY FORTE
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Food Samples ◽  
Vibrio Alginolyticus ◽  
Pcr Assay ◽  
Molecular Approaches ◽  
Multiplex Pcr Assay ◽  
Primer Sets ◽  
Species Specific

A multiplex PCR assay using three collagenase-targeted primer pairs for the species-specific detection of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus was developed. The results highlight the species specificity of the three primer sets designed. Because of the increasing importance of Vibrio spp. in human foodborne diseases, molecular approaches for routine microbial screening and monitoring of clinical, environmental, and food samples also have become more important. The results of this study indicate that the gene coding for collagenase should be used as an alternative molecular target to discriminate among the three Vibrio species.

scholarly journals Occurrence, Diversity, and Pathogenicity of HalophilicVibrio spp. and Non-O1 Vibrio cholerae from Estuarine Waters along the Italian Adriatic Coast

1999 ◽  
Vol 65 (6) ◽  
pp. 2748-2753 ◽  
Author(s):  
Elena Barbieri ◽  
Loredana Falzano ◽  
Carla Fiorentini ◽  
Anna Pianetti ◽  
Wally Baffone ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Vibrio Alginolyticus ◽  
Estuarine Waters ◽  
Predominant Species ◽  
Biochemical Fingerprinting ◽  
Fingerprinting Method ◽  
Adriatic Coast

ABSTRACT The occurrence, diversity, and pathogenicity of Vibriospp. were investigated in two estuaries along the Italian Adriatic coast. Vibrio alginolyticus was the predominant species, followed by Vibrio parahaemolyticus, non-O1 Vibrio cholerae, and Vibrio vulnificus. By using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. The production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number of Vibrio strains. These findings indicate a significant presence of potentially pathogenicVibrio strains along the Adriatic coast.

Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

2011 ◽  
Vol 25 (4) ◽  
pp. 174-176 ◽  
Author(s):  
Hidemasa Izumiya ◽  
Kazutoshi Matsumoto ◽  
Shunsuke Yahiro ◽  
Jiyoung Lee ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

scholarly journals Light-scattering sensor for real-time identification of Vibrio parahaemolyticus , Vibrio vulnificus and Vibrio cholerae colonies on solid agar plate

2012 ◽  
Vol 5 (5) ◽  
pp. 607-620 ◽  
Author(s):  
Karleigh Huff ◽  
Amornrat Aroonnual ◽  
Amy E. Fleishman Littlejohn ◽  
Bartek Rajwa ◽  
Euiwon Bae ◽  
...  
Keyword(s):  
Light Scattering ◽  
Vibrio Cholerae ◽  
Real Time ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Agar Plate ◽  
Solid Agar ◽  
Real Time Identification

Occurrence of Vibrio vulnificus and Toxigenic Vibrio parahaemolyticus on Sea Catfishes from Galveston Bay, Texas

2014 ◽  
Vol 77 (10) ◽  
pp. 1784-1786 ◽  
Author(s):  
LESLIE BAUMEISTER ◽  
MONA E. HOCHMAN ◽  
JOHN R. SCHWARZ ◽  
ROBIN BRINKMEYER
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Life Histories ◽  
Vibrio Vulnificus ◽  
Trophic Levels ◽  
Human Consumption ◽  
Galveston Bay ◽  
Seafood Consumption ◽  
Increasing Trend ◽  
Pcr Assays ◽  
Tdh Gene

Dorsal and pectoral fin spines from two species of sea catfishes (Bagre marinus and Ariopsis felis) landed at 54 sites in Galveston Bay, Texas, and its subbays from June to October 2005 were screened with traditional cultivation-based assays and quantitative PCR assays for Vibrio vulnificus and Vibrio parahaemolyticus. V. vulnificus was present on 51.2% of fish (n = 247), with an average of 403 ±337 SD cells g−1. V. parahaemolyticus was present on 94.2% (n = 247); 12.8% tested positive for the virulence-conferring tdh gene, having an average 2,039 ± 2,171 SD cells g−1. The increasing trend in seafood consumption of “trash fishes” from lower trophic levels, such as sea catfishes, warrants evaluation of their life histories for association with pathogens of concern for human consumption.

Occurrence of Vibrios in Frozen Seafoods and Survival of Psychrotrophic Vibrio cholerae in Broth and Shrimp Homogenate at Low Temperatures

1995 ◽  
Vol 58 (3) ◽  
pp. 263-267 ◽  
Author(s):  
HIN-CHUNG WONG ◽  
LI-LI CHEN ◽  
CHUNG-MING YU
Keyword(s):  
Sodium Chloride ◽  
Vibrio Cholerae ◽  
Low Temperatures ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Alginolyticus ◽  
Cold Injury ◽  
Tropical Regions ◽  
Etiologic Agents ◽  
Pathogenic Vibrios

Pathogenic vibrios are important etiologic agents in tropical regions and have been frequently recovered from seafoods and aquacultured foods. In this study, commercially frozen seafoods including peeled shrimps and fish and shrimp dumplings were examined. Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio cholerae and Vibria fluvialis were recovered at 36.0%, 15.8%, 14.9% and 13.2%, respectively. A number of psychrotrophic vibrios were selected and their survival in tryptic soy broth (TSB) supplemented with 1% sodium chloride (NaCl) (TSBS medium) and shrimp homogenate at 4°C and −30°C were studied. Two psychrotrophic non-O1 V. cholerae (laboratory stocks no 128 and 129) survived well at these low temperatures. Counts decreased by about 1 log/ml in TSBS medium at 4°C for 6 days and 3 log/ml at −30°C for 3 days. Shrimp homogenate provided better protection than TSBS medium for psychrotrophic V. cholerae at −30°C. Survival of V. cholerae at low temperatures was further increased by the addition of 0.5% of heated pyrophosphate and metaphosphate, probably by decreasing the lethality of the cold injury to the cells. Measures should be taken to minimize the risk from pathogenic vibrios in frozen seafoods, especially if phosphates are used and psychrotrophic strains are present.

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