Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

2011 ◽  
Vol 25 (4) ◽  
pp. 174-176 ◽  
Author(s):  
Hidemasa Izumiya ◽  
Kazutoshi Matsumoto ◽  
Shunsuke Yahiro ◽  
Jiyoung Lee ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

A Collagenase-Targeted Multiplex PCR Assay for Identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus

2005 ◽  
Vol 68 (1) ◽  
pp. 150-153 ◽  
Author(s):  
ANGELA DI PINTO ◽  
GIUSEPPINA CICCARESE ◽  
GIUSEPPINA TANTILLO ◽  
DOMENICO CATALANO ◽  
VITO TONY FORTE
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Food Samples ◽  
Vibrio Alginolyticus ◽  
Pcr Assay ◽  
Molecular Approaches ◽  
Multiplex Pcr Assay ◽  
Primer Sets ◽  
Species Specific

A multiplex PCR assay using three collagenase-targeted primer pairs for the species-specific detection of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus was developed. The results highlight the species specificity of the three primer sets designed. Because of the increasing importance of Vibrio spp. in human foodborne diseases, molecular approaches for routine microbial screening and monitoring of clinical, environmental, and food samples also have become more important. The results of this study indicate that the gene coding for collagenase should be used as an alternative molecular target to discriminate among the three Vibrio species.

scholarly journals Multiplex PCR Assay for Detection of Vibrio vulnificus Biotype 2 and Simultaneous Discrimination of Serovar E Strains

2007 ◽  
Vol 73 (6) ◽  
pp. 2029-2032 ◽  
Author(s):  
Eva Sanjuán ◽  
Carmen Amaro
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Vulnificus ◽  
Simultaneous Discrimination ◽  
Fish Pathogen ◽  
Pcr Assay ◽  
Culture Collections ◽  
Multiplex Pcr Assay ◽  
Detection And Identification ◽  
Field Samples ◽  
Zoonotic Strains

ABSTRACT In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.

A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas

2021 ◽  
Vol 55 ◽  
pp. 101689
Author(s):  
Hongxia Guan ◽  
Panpan Xue ◽  
Haijian Zhou ◽  
Dan Sha ◽  
Duochun Wang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Species ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio

Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups

2019 ◽  
Vol 157 ◽  
pp. 54-58 ◽  
Author(s):  
Sharda Prasad Awasthi ◽  
Nityananda Chowdhury ◽  
Sucharit Basu Neogi ◽  
Atsushi Hinenoya ◽  
Noritoshi Hatanaka ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Virulence Genes ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan

2015 ◽  
Vol 78 (5) ◽  
pp. 969-976 ◽  
Author(s):  
YAO HSIEN TEY ◽  
KOA-JEN JONG ◽  
SHIN-YUAN FEN ◽  
HIN-CHUNG WONG
Keyword(s):  
Water Temperature ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Membrane Filtration ◽  
Vibrio Vulnificus ◽  
Pearson Correlation ◽  
Isolation Frequency ◽  
Freshwater Ponds ◽  
Pathogenic Vibrio ◽  
Southern Taiwan

The occurrence of Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern Taiwan was examined by the membrane filtration and colony hybridization method. The halophilic V. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log CFU/g. V. cholerae was found in both the seawater and freshwater ponds but preferentially in freshwater ponds, with a frequency of 72.2% and a mean density of 1.65 log CFU/g. V. vulnificus was identified mainly in seawater ponds, with an isolation frequency of 27.8%. The density of V. parahaemolyticus in seawater ponds was positively related to water temperature (Pearson correlation coefficient, r = 0.555) and negatively related to salinity (r = −0.333). The density of V. cholerae in all six ponds was positively related to water temperature (r = 0.342) and negatively related to salinity (r = −0.432). Two putatively pathogenic tdh+ V. parahaemolyticus isolates (1.4% of the samples) and no ctx+ V. cholerae isolates were identified. The experimental results may facilitate assessments of the risk posed by these pathogenic Vibrio species in Taiwan, where aquaculture provides a large part of the seafood supply.

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