scholarly journals Replication fidelity in E. coli: Differential leading and lagging strand effects for dnaE antimutator alleles

DNA Repair ◽  
2019 ◽  
Vol 83 ◽  
pp. 102643
Author(s):  
Karolina Makiela-Dzbenska ◽  
Katarzyna H. Maslowska ◽  
Wojciech Kuban ◽  
Damian Gawel ◽  
Piotr Jonczyk ◽  
...  
Keyword(s):  
Replication Fidelity ◽  
E Coli ◽  
Lagging Strand

scholarly journals Near-continuously synthesized leading strands inEscherichia coliare broken by ribonucleotide excision

2019 ◽  
Vol 116 (4) ◽  
pp. 1251-1260 ◽  
Author(s):  
Glen E. Cronan ◽  
Elena A. Kouzminova ◽  
Andrei Kuzminov
Keyword(s):  
Dna Replication ◽  
Excision Repair ◽  
Chromosomal Dna ◽  
E Coli ◽  
Okazaki Fragments ◽  
Leading Strand ◽  
Replication Intermediates ◽  
Lagging Strand

In vitro, purified replisomes drive model replication forks to synthesize continuous leading strands, even without ligase, supporting the semidiscontinuous model of DNA replication. However, nascent replication intermediates isolated from ligase-deficientEscherichia colicomprise only short (on average 1.2-kb) Okazaki fragments. It was long suspected that cells replicate their chromosomal DNA by the semidiscontinuous mode observed in vitro but that, in vivo, the nascent leading strand was artifactually fragmented postsynthesis by excision repair. Here, using high-resolution separation of pulse-labeled replication intermediates coupled with strand-specific hybridization, we show that excision-proficientE. coligenerates leading-strand intermediates >10-fold longer than lagging-strand Okazaki fragments. Inactivation of DNA-repair activities, including ribonucleotide excision, further increased nascent leading-strand size to ∼80 kb, while lagging-strand Okazaki fragments remained unaffected. We conclude that in vivo, repriming occurs ∼70× less frequently on the leading versus lagging strands, and that DNA replication inE. coliis effectively semidiscontinuous.

scholarly journals Mismatch Repair Balances Leading and Lagging Strand DNA Replication Fidelity

PLoS Genetics ◽  
2012 ◽  
Vol 8 (10) ◽  
pp. e1003016 ◽  
Author(s):  
Scott A. Lujan ◽  
Jessica S. Williams ◽  
Zachary F. Pursell ◽  
Amy A. Abdulovic-Cui ◽  
Alan B. Clark ◽  
...  
Keyword(s):  
Dna Replication ◽  
Mismatch Repair ◽  
Replication Fidelity ◽  
Lagging Strand ◽  
Dna Replication Fidelity

scholarly journals Mechanism of the E. coli τ Processivity Switch during Lagging-Strand Synthesis

2003 ◽  
Vol 11 (2) ◽  
pp. 315-327 ◽  
Author(s):  
Frank P Leu ◽  
Roxana Georgescu ◽  
Mike O'Donnell
Keyword(s):  
E Coli ◽  
Lagging Strand

Preferential DNA secondary structure mutagenesis in the lagging strand of replication in E. coli

Nature ◽  
1991 ◽  
Vol 352 (6335) ◽  
pp. 544-547 ◽  
Author(s):  
Thuan Q Trinh ◽  
Richard R Sinden
Keyword(s):  
Secondary Structure ◽  
Dna Secondary Structure ◽  
E Coli ◽  
Lagging Strand

scholarly journals Staphylococcus aureus Helicase but Not Escherichia coli Helicase Stimulates S. aureus Primase Activity and Maintains Initiation Specificity

2006 ◽  
Vol 188 (13) ◽  
pp. 4673-4680 ◽  
Author(s):  
Scott A. Koepsell ◽  
Marilynn A. Larson ◽  
Mark A. Griep ◽  
Steven H. Hinrichs
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Dna Replication ◽  
Full Length ◽  
Recognition Sequence ◽  
Dna Templates ◽  
E Coli ◽  
The Third ◽  
Leading Strand ◽  
Lagging Strand

ABSTRACT Bacterial primases are essential for DNA replication due to their role in polymerizing the formation of short RNA primers repeatedly on the lagging-strand template and at least once on the leading-strand template. The ability of recombinant Staphylococcus aureus DnaG primase to utilize different single-stranded DNA templates was tested using oligonucleotides of the sequence 5′-CAGA (CA)5 XYZ (CA)3-3′, where XYZ represented the variable trinucleotide. These experiments demonstrated that S. aureus primase synthesized RNA primers predominately on templates containing 5′-d(CTA)-3′ or TTA and to a much lesser degree on GTA-containing templates, in contrast to results seen with the Escherichia coli DnaG primase recognition sequence 5′-d(CTG)-3′. Primer synthesis was initiated complementarily to the middle nucleotide of the recognition sequence, while the third nucleotide, an adenosine, was required to support primer synthesis but was not copied into the RNA primer. The replicative helicases from both S. aureus and E. coli were tested for their ability to stimulate either S. aureus or E. coli primase. Results showed that each bacterial helicase could only stimulate the cognate bacterial primase. In addition, S. aureus helicase stimulated the production of full-length primers, whereas E. coli helicase increased the synthesis of only short RNA polymers. These studies identified important differences between E. coli and S. aureus related to DNA replication and suggest that each bacterial primase and helicase may have adapted unique properties optimized for replication.

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Ribosome Structural Organization

1974 ◽  
Vol 32 ◽  
pp. 332-333
Author(s):  
James A. Lake
Keyword(s):  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Small Subunit ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
Specific Antibodies ◽  
X Ray ◽  
E Coli ◽  
Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

Sign in / Sign up

Export Citation Format

Share Document