A bench-scale denitrification wall for simulating the in-situ treatment of nitrate-contaminated groundwater

This paper reviews ten years of research on on-site and in situ bioremediation of chlorophenol contaminated groundwater. Laboratory experiments on the development of a high-rate, fluidized-bed process resulted in a full-scale, pump-and-treat application which has operated for several years. The system operates at ambient groundwater temperature of 7 to 9°C at 2.7 d hydraulic retention time and chlorophenol removal efficiencies of 98.5 to 99.9%. The microbial ecology studies of the contaminated aquifer revealed a diverse chlorophenol-degrading community. In situ biodegradation of chlorophenols is controlled by oxygen availability, only. Laboratory and pilot-scale experiments showed the potential for in situ aquifer bioremediation with iron oxidation and precipitation as a potential problem.

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A bioengineering system for in situ bioremediation of contaminated groundwater

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.2900321 ◽

1997 ◽

Vol 18 (2-3) ◽

pp. 189-197 ◽

Cited By ~ 13

Author(s):

R B Knapp ◽

B D Faison

Keyword(s):

Contaminated Groundwater ◽

In Situ Bioremediation

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Peer Reviewed: In-Situ Treatment of Chromium-Contaminated Groundwater

Environmental Science & Technology ◽

10.1021/es022466i ◽

2002 ◽

Vol 36 (23) ◽

pp. 464A-472A ◽

Cited By ~ 68

Author(s):

Jonathan Fruchter

Keyword(s):

Contaminated Groundwater

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Optimal Initial Configuration of Treatment Solution for In Situ Remediation of Contaminated Groundwater Using Engineered Injection and Extraction

World Environmental and Water Resources Congress 2013 ◽

10.1061/9780784412947.252 ◽

2013 ◽

Author(s):

Julia Traylor

Keyword(s):

Initial Configuration ◽

Contaminated Groundwater ◽

In Situ Remediation

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Optimasi Proses Epoksidasi Asam Oleat pada Scaling Up Sintesis Asam 9,10-Dihidroksi Stearat (DHSA) Bench Scale

Majalah ilmiah Pengkajian Industri ◽

10.29122/mipi.v12i1.2616 ◽

2018 ◽

Vol 12 (1) ◽

pp. 39-46

Author(s):

Maisaroh Maisaroh

Keyword(s):

Engineering Design ◽

Scaling Up ◽

Bench Scale

Sintesis asam 9,10-dihidroksi stearat (DHSA) dari asam oleat terepoksidasi merupakan salah satu upaya yang akan meningkatkan penggunaan, diversifikasi dan nilai tambah minyak kelapa sawit. Scaling up proses epoksidasi asam oleat dari skala laboratorium ke bench scale (kapasitas 5 L) terjadi perubahan volume dan geometri dari peralatan yang akan mempengaruhi proses epoksidasi itu sendiri sehingga perlu dilakukan observasi terhadap parameter-parameter yang akan digunakan dalam basic dan engineering design. Tujuan dari penelitian ini adalah melakukan scaling up proses konsistensi dari skala laboratorium ke bench scale (kap. 5 L) epoksidasi asam oleat sebagai produk antara sintesis DHSA dalam pengembangan produk turunan kelapa sawit yang akan digunakan sebagai bahan kosmetik. Scaling Up epoksidasi asam oleat dengan asam performat yang dibentuk secara in situ dilakukan menggunakan reaktor 5 liter dengan perbandingan mol asam oleat : asam format : hidrogen peroksida 50% = 1 : 1,25 : 6. Produk epoksidasi ini akan dilanjutkan untuk dihidroksilasi pada sintesis DHSA sebagai bahan kosmetik.

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Potential forMethanosarcinato contribute to uranium reduction during acetate-promoted groundwater bioremediation

10.1101/202242 ◽

2017 ◽

Author(s):

Dawn E Holmes ◽

Roberto Orelana ◽

Ludovic Giloteaux ◽

Li-Ying Wang ◽

Pravin Shrestha ◽

...

Keyword(s):

Field Experiment ◽

Electron Donor ◽

Enrichment Culture ◽

Cell Suspensions ◽

Contaminated Groundwater ◽

Subunit A ◽

Sedimentary Environments

AbstractPrevious studies ofin situbioremediation of uranium-contaminated groundwater with acetate injections have focused on the role ofGeobacterspecies in U(VI) reduction because of a lack of other abundant known U(VI)-reducing microorganisms. Monitoring the levels of methyl CoM reductase subunit A (mcrA) transcripts during an acetate-injection field experiment demonstrated that acetoclastic methanogens from the genusMethanosarcinawere enriched after 40 days of acetate amendment. The increased abundance ofMethanosarcinacorresponded with an accumulation of methane in the groundwater. An enrichment culture dominated by aMethanosarcinaspecies with the sameMethanosarcina mcrAsequence that predominated in the field experiment could effectively convert acetate to methane. In order to determine whetherMethanosarcinaspecies could be participating in U(VI) reduction in the subsurface, cell suspensions ofM. barkeriwere incubated in the presence of U(VI) with acetate provided as the electron donor. U(VI) was reduced by metabolically activeM. barkericells, however, no U(VI) reduction was observed in inactive controls. These results demonstrate thatMethanosarcinaspecies could play an important role in the long-term bioremediation of uranium-contaminated aquifers after depletion of Fe(III) oxides limits the growth ofGeobacterspecies. The results also suggest thatMethanosarcinahave the potential to influence uranium geochemistry in a diversity of anaerobic sedimentary environments.

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Feasibility study of in-situ bioremediation for nitrobenzene-contaminated groundwater

Water Science & Technology Water Supply ◽

10.2166/ws.2017.014 ◽

2017 ◽

Vol 17 (4) ◽

pp. 1160-1167 ◽

Cited By ~ 2

Author(s):

Na Liu ◽

Yue Wang ◽

Yonglei An ◽

Feng Ding ◽

Xiaolong Yu ◽

...

Keyword(s):

Simulation Experiment ◽

Laboratory Simulation ◽

Contaminated Groundwater ◽

Site Conditions ◽

In Situ Bioremediation ◽

Degrading Bacterium ◽

Bacterium Strain ◽

Negative Factors ◽

Sufficient Oxygen

Although many studies have simulated in-situ bioremediation of contaminated groundwater, most of them have not considered hydrochemical conditions and indigenous microorganisms, thus potentially rendering results inapplicable to actual in-situ groundwater bioremediation projects. This study focused on a nitrobenzene-contaminated groundwater site located in Jilin City, China. The actual nitrobenzene-contaminated groundwater was taken from Jilin City to simulate in-situ groundwater bioremediation in the laboratory. The feasibility of in-situ bioremediation for nitrobenzene-contaminated groundwater was studied according to actual site conditions and characteristics of nitrobenzene-degrading microorganisms in groundwater. The results showed that nitrobenzene-degrading bacterium strain NB1 was the dominant species that could effectively and rapidly degrade nitrobenzene by a partial reductive pathway. No negative factors on the growth or degrading function of this strain in groundwater could be detected. During a laboratory simulation experiment, combined in-situ bioremediation technologies, namely air sparging and bioaugmentation, could readily remove approximately 89.56% of nitrobenzene from groundwater without adding nutrients; oxygen was found to be the important growth factor for strain NB1. As the substrate of nitroreductase, encoded by the nitrobenzene nitroreductase (nbzA) gene, nitrobenzene was likely to significantly affect the expression of this gene. In conclusion, in-situ bioremediation is a feasible way to solve the problem of nitrobenzene-contaminated groundwater in Jilin City as long as sufficient oxygen and biomass of strain NB1 is ensured.

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