Fermentation of xylose, arabinose, glucose, their mixtures and sugarcane bagasse hydrolyzate by yeast Pichia stipitis for ethanol production

AbstractThe search for new ethanol production technologies is due to this biofuel being a renewable and environmentally friendly option. Immobilized cell systems for ethanol production have been studied; however, the phenomenon involved in cell sorption on raw materials has been poorly explored. Therefore, this work evaluates P. stipitis immobilization on sugarcane bagasse pretreated with sulphuric acid, as well as ethanol production in batch culture. The results obtained showed that the Guggenheim-Anderson-de Boer (GAB) model explained the sorption phenomenon. The selected inoculum size for immobilization was the same as the monolayer sorption capability (1.17 gl-1). Using 1:100 g ml- 1 solid-liquid ratio, at 250 rpm, ethanol yield and productivity of 0.404 gg-1 glucose and 0.41 gl-1h-1 were obtained, respectively. The immobilized systems were stable for up to twenty-five repeated batches (36 h each). Ethanol production was increased from the first to the twenty-fifth batch (18.1 and 24.7 gl-1 ethanol). The use of complex media, such as molasses “B” or sugarcane hydrolyzates, caused an increase in process efficiency 2.4 and 1.8-fold respectively, compared with free cells systems. Biotechnological ethanol production from lignocellulosic hydrolyzates could be improved by the use of the immobilization cell sorption on pre-treated raw materials.

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Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells of Pichia stipitis NCIM 3498

International Journal of Chemical Reactor Engineering ◽

10.2202/1542-6580.2514 ◽

2011 ◽

Vol 9 (1) ◽

Author(s):

Chandrasekhar Gajula ◽

Anuj Kumar Chandel ◽

Radhika Konakalla ◽

Ravinder Rudravaram ◽

Ravindra Pogaku ◽

...

Keyword(s):

Ethanol Production ◽

Immobilized Cells ◽

Pichia Stipitis ◽

Fuel Ethanol ◽

Enzymatic Hydrolysate ◽

Groundnut Shell

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The water consumption of sugarcane bagasse post-washing after protic ionic liquid pretreatment and its impact on 2G ethanol production

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113642 ◽

2021 ◽

Vol 169 ◽

pp. 113642

Author(s):

P.Y.S. Nakasu ◽

J.L. Ienczak ◽

S.C. Rabelo ◽

A.C. Costa

Keyword(s):

Ionic Liquid ◽

Ethanol Production ◽

Sugarcane Bagasse ◽

Water Consumption ◽

Protic Ionic Liquid ◽

Ionic Liquid Pretreatment

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Ethanol Production from Corn Stover Using Soaking Pretreatment

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.171-172.261 ◽

2010 ◽

Vol 171-172 ◽

pp. 261-265

Author(s):

Zhuang Zuo ◽

Xiu Shan Yang

Keyword(s):

Ethanol Production ◽

Corn Stover ◽

Simultaneous Saccharification And Fermentation ◽

Pichia Stipitis ◽

Liquid Ratio ◽

Mild Conditions ◽

Whole Process ◽

Conversion Rates ◽

Mild Temperature ◽

Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% deligniﬁcation, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous sacchariﬁcation and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

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