scholarly journals Ethanol production by Pichia stipitis immobilized on sugarcane bagasse

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
BS Yñiguez-Balderas ◽  
B Ortiz-Muñiz ◽  
J Gómez-Rodríguez ◽  
B Gutierrez-Rivera ◽  
MG Aguilar-Uscanga
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Ethanol Yield ◽  
Raw Materials ◽  
Pichia Stipitis ◽  
Inoculum Size ◽  
Process Efficiency ◽  
Immobilized Cell ◽  
Production Technologies ◽  
Gab Model

AbstractThe search for new ethanol production technologies is due to this biofuel being a renewable and environmentally friendly option. Immobilized cell systems for ethanol production have been studied; however, the phenomenon involved in cell sorption on raw materials has been poorly explored. Therefore, this work evaluates P. stipitis immobilization on sugarcane bagasse pretreated with sulphuric acid, as well as ethanol production in batch culture. The results obtained showed that the Guggenheim-Anderson-de Boer (GAB) model explained the sorption phenomenon. The selected inoculum size for immobilization was the same as the monolayer sorption capability (1.17 gl-1). Using 1:100 g ml- 1 solid-liquid ratio, at 250 rpm, ethanol yield and productivity of 0.404 gg-1 glucose and 0.41 gl-1h-1 were obtained, respectively. The immobilized systems were stable for up to twenty-five repeated batches (36 h each). Ethanol production was increased from the first to the twenty-fifth batch (18.1 and 24.7 gl-1 ethanol). The use of complex media, such as molasses “B” or sugarcane hydrolyzates, caused an increase in process efficiency 2.4 and 1.8-fold respectively, compared with free cells systems. Biotechnological ethanol production from lignocellulosic hydrolyzates could be improved by the use of the immobilization cell sorption on pre-treated raw materials.

scholarly journals Robustness and Ethanol Production of Industrial Strains of Saccharomyces cerevisiae Using Different Sugarcane Bagasse Hydrolysates

2019 ◽  
Vol 7 (1) ◽  
pp. 23 ◽  
Author(s):  
Vanessa S. Teixeira ◽  
Suéllen P. H. Azambuja ◽  
Priscila H. Carvalho ◽  
Fátima A. A. Costa ◽  
Patricia R. Kitaka ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Ethanol Yield ◽  
Raw Materials ◽  
Fermentation Inhibitors ◽  
Severe Condition ◽  
Inhibitor Tolerance ◽  
Industrial Strains ◽  
Materials Used

Sugarcane bagasse is one of the main lignocellulosic raw materials used for the production of second-generation ethanol. Technological studies on fermentation processes have focused on the search for and development of more robust microorganisms that are able to produce bioethanol efficiently and are resistant to the main fermentation inhibitors. The purpose of this study was to evaluate the robustness and ethanol production of industrial strains of Saccharomyces cerevisiae using acid, alkaline, and enzymatic sugarcane bagasse hydrolysates. Hydrolysis was carried out to release fermentable sugars from sugarcane bagasse. Fermentations were performed in shake flasks containing sugarcane hydrolysates supplemented with 150 g L−1 glucose to evaluate the kinetic parameters of the reaction. Inhibitor tolerance was evaluated by incubating cells with different concentrations of inhibitors in 96-well plates. The biomass yield on substrate, ethanol yield on substrate, and ethanol productivity of the six strains were higher in 0.5% acid, 0.5% alkaline, and enzymatic hydrolysates (i.e., under milder conditions). The SA-1 (Santa Adélia-1) strain had a better performance in comparison with the other strains for its ability to produce ethanol in a very severe condition (7% acid hydrolysis) and for its robustness in growing at several inhibitor concentrations.

scholarly journals Direct Ethanol Production from Lignocellulosic Sugars and Sugarcane Bagasse by a RecombinantTrichoderma reeseiStrain HJ48

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jun Huang ◽  
Dong Chen ◽  
Yutuo Wei ◽  
Qingyan Wang ◽  
Zhenchong Li ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Trichoderma Reesei ◽  
Sugarcane Bagasse ◽  
Consolidated Bioprocessing ◽  
Ethanol Yield ◽  
Genome Shuffling ◽  
Wild Type ◽  
Mutant Population ◽  
Ethanol Resistance ◽  
Lignocellulosic Sugars

Trichoderma reeseican be considered as a candidate for consolidated bioprocessing (CBP) microorganism. However, its ethanol yield needs to be improved significantly. Here the ethanol production ofT. reeseiCICC 40360 was improved by genome shuffling while simultaneously enhancing the ethanol resistance. The initial mutant population was generated by nitrosoguanidine treatment of the spores, and an improved population producing more than fivefold ethanol than wild type was obtained by genome shuffling. The results show that the shuffled strain HJ48 can efficiently convert lignocellulosic sugars to ethanol under aerobic conditions. Furthermore, it was able to produce ethanol directly from sugarcane bagasse, demonstrating that the shuffled strain HJ48 is a suitable microorganism for consolidated bioprocessing.

scholarly journals Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Wan ◽  
Dongmei Zhai ◽  
Zhen Wang ◽  
Xiushan Yang ◽  
Shen Tian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Synthetic Medium ◽  
Pichia Stipitis ◽  
Dilute Acid ◽  
Acid Hydrolysate ◽  
Issatchenkia Orientalis ◽  
Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

QUALIDADE DO CALDO PARA PRODUÇÃO DE ETANOL EM CULTIVARES DE SORGO SACARINO SOB ADUBAÇÃO NITROGENADA

2017 ◽  
Vol 16 (3) ◽  
pp. 388
Author(s):  
CRISTÓBAL SOTO SOLANO ◽  
NIRALDO JOSÉ PONCIANO ◽  
ROGÉRIO FIGUEIREDO DAHER ◽  
RAFAEL AUGUSTO DA COSTA ◽  
PAULO MARCELO DE SOUZA ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Nitrogen Fertilization ◽  
Ethanol Yield ◽  
Raw Materials ◽  
Raw Material ◽  
Maximum Value ◽  
Total Reducing Sugars ◽  
Nitrogen Rates

 RESUMO - O sorgo sacarino tem potencial de uso como fonte de matéria-prima para a produção de bioetanol. Objetivou-se, com este estudo, analisar o efeito da adubação nitrogenada de cobertura na qualidade do caldo para produção de etanol de quatro cultivares de sorgo sacarino. O experimento foi conduzido em Campos dos Goytacazes- RJ, de janeiro a maio de 2014. Foram estudadas quatro cultivares de sorgo sacarino: BRS 506, BRS 508, BRS 509 e BRS 511 e cinco de doses de nitrogênio (0, 80, 160, 240 e 320 Kg ha-1), aplicadas aos 25 e 45 dias após semeadura. Os resultados mostraram que valor Brix, açúcares redutores totais, açúcares totais recuperáveis, volume do caldo e rendimento de etanol foram afetados pela adubação nitrogenada. Para todas as características, o valor máximo foi obtido pela dose de 240 kg de N ha-1. Entre as cultivares, a BRS 506 mostrou-se superior em produção de caldo e rendimento de etanol. Enquanto a BRS 511 destacou-se nos atributos ART e ATR, e a cultivar BRS 508 sobressaiu-se no valor Brix. A cultivar BRS 506 apresentou melhor resultado combinado, com maior volume de caldo e rendimento de etanol com as doses de 204 e 212 kg N ha-1, respectivamente. Palavras-chave: biocombustíveis, rendimento de álcool carburante, atributos industriais, caracterização da matéria prima, genótipos. QUALITY OF BROTH FOR ETHANOL PRODUCTION IN CULTIVARS OF SACARINE SORGHUM UNDER NITROGEN FERTILIZATION  ABSTRACT - Sweet sorghum has potential as a source of raw material for the production of bioethanol. The objective of this study was to analyze the effect of nitrogen topdressing on the quality of the juice for ethanol production from four cultivars of sorghum. The experiment was conducted in Campos, State of Rio de Janeiro, Brazil, from January to May 2014. Four cultivars of sorghum: BRS 506, BRS 508, BRS 509 and BRS 511 were studied, and five nitrogen rates (0, 80, 160, 240 and 320 kg ha-1), applied to 25 and 45 days after sowing. The results showed that the value Brix, total reducing sugars, total recoverable sugars, broth volume and ethanol yield were affected by nitrogen fertilization. For all features, the maximum value was obtained for the dose of 240 kg N ha-1. Among cultivars, BRS 506 was superior in production of broth and ethanol yield. BRS 511 stood out in the attributes ART and ATR, and BRS 508, in value Brix. BRS 506 had better combined result of higher volume of juice and ethanol yield with doses of 204 and 212 kg N ha-1, respectively Keywords: biofuels, fuel ethanol output, industrial attributes, characterization of raw materials, genotypes.   

Ethanol Production from Sugarcane Bagasse Hydrolysate Using Pichia stipitis

2009 ◽  
Vol 161 (1-8) ◽  
pp. 84-92 ◽  
Author(s):  
Larissa Canilha ◽  
Walter Carvalho ◽  
Maria das Graças de Almeida Felipe ◽  
João Batista de Almeida e Silva ◽  
Marco Giulietti
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Pichia Stipitis ◽  
Sugarcane Bagasse Hydrolysate

Proper ultrasound treatment increases ethanol production from simultaneous saccharification and fermentation of sugarcane bagasse

RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91409-91419 ◽  
Author(s):  
Rajendran Velmurugan ◽  
Aran Incharoensakdi
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Ultrasound Treatment ◽  
Fermentation Processes ◽  
Cellulase Complex ◽  
Simultaneous Saccharification

To improve the saccharification and fermentation processes, proper ultrasound was applied which resulted in the presence of cellulase complex with improved β-glucosidase ratio leading to enhanced overall ethanol yield.

scholarly journals Fermentation of xylose, arabinose, glucose, their mixtures and sugarcane bagasse hydrolyzate by yeast Pichia stipitis for ethanol production

2020 ◽  
Vol 6 ◽  
pp. 710-713 ◽  
Author(s):  
Natthanon Phaiboonsilpa ◽  
Teerin Chysirichote ◽  
Verawat Champreda ◽  
Navadol Laosiripojana
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Pichia Stipitis

scholarly journals Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis

2016 ◽  
Vol 2 (1) ◽  
pp. 1-12
Author(s):  
Inda Setyawati ◽  
Laksmi Ambarsari ◽  
Siti Nur'aeni ◽  
Suryani Suryani ◽  
Puspa Julistia Puspita ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Ethanol Yield ◽  
Renewable Resource ◽  
Bioethanol Production ◽  
Fermentation Inhibitors ◽  
Renewable Materials ◽  
Promising Alternative ◽  
Fermentation Time ◽  
Sugarcane Bagasse Hydrolysate

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.

scholarly journals Potential Utilization of Low Quality Sweet Potato for Bioethanol Production by Saccharomyces cerevisiae TISTR5339

2020 ◽  
Vol 17 (9) ◽  
pp. 933-946
Author(s):  
Supasit CHOOKLIN ◽  
Parichat NINUP-PATHAM ◽  
Saovanee CHOOJIT
Keyword(s):  
Ethanol Production ◽  
Acid Hydrolysis ◽  
Sweet Potato ◽  
Ammonium Sulphate ◽  
Ethanol Yield ◽  
Scale Up ◽  
Inoculum Size ◽  
Sulphate Content ◽  
Box Behnken Design ◽  
Hydrolysis Of

This study was aimed to investigate the optimal condition of ethanol production that has 2 major stages: acid hydrolysis and fermentation processes. These processes came from low quality sweet potato (LQSP) which was destroyed by the sweet potato weevil. The main compositions of LQSP were starch and fiber which consist of 55.25 and 10.29 %, respectively. In this case, the starch can be hydrolyzed to reduce the sugar, followed by the fermentation of the reduced sugar to ethanol. For this experiment, the effecting factors on acid hydrolysis of LQSP and the ethanol fermentation condition were optimized by S. cerevisiae using Response Surface Methodology (RSM) with Box-Behnken design in order to maximize ethanol yield. It was found that the maximum reducing sugar concentration of 390.99 ± 5.35 g/L was obtained from the hydrolysis condition with 1 % (v/v) of sulfuric acid and 25 % (w/v) of LQSP. Accordingly, the effects of ammonium sulphate content (0.05 - 0.15 %), pH (4.5 - 5.5) and inoculum content (5 - 10 %) on ethanol production was determined by RSM using Box-Behnken experiment design with a total 17 sets of all trials. The results were found that the maximum experimental ethanol productivity of 5.98 g/L was obtained from the condition at 0.05 % of ammonium sulphate, pH 5.5 and 5.0 % of inoculum size to 90 mL LQSP based medium and incubated at 30 °C for 48 h. In addition, the scale-up of ethanol production was studied in 9 L fermenter which provided the maximum ethanol yield of 5.04 g/L. Therefore, it can be concluded that LQSP had a potential as a substrate for ethanol production.

scholarly journals High yield of ethanol production by the strain Fusarium sp. ZW-21 with corncob hydrolysate

BioResources ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 622-632
Author(s):  
Xiaohui Liang ◽  
Hongyu Si ◽  
Dongliang Hua ◽  
Yuxiao Zhao
Keyword(s):  
Ethanol Production ◽  
Yeast Extract ◽  
Incubation Temperature ◽  
Ethanol Yield ◽  
Inoculum Size ◽  
Effect Of Temperature ◽  
High Yield ◽  
Polysorbate 80 ◽  
Corncob Hydrolysate ◽  
Fusarium Sp

The filamentous fungus strain Fusarium sp. ZW-21 was used for ethanol production with corncob hydrolysate. The fermentation conditions of ethanol production from corncob hydrolysate by the strain were investigated, and the effect of temperature, pH, nitrogen source, and surfactants on the ethanol production was investigated. The two factors yeast extract and polysorbate 80 were selected for further optimization by response surface methodology. The optimal conditions for ethanol production by the strain Fusarium sp. ZW-21 were 50 g/L sugar of corncob hydrolysate, 10.35 g/L yeast extract, 10 g/L KH2PO4, 0.5 g/L MgSO4·7H2O, 0.38 g/L polysorbate 80, pH 6.0, inoculum size of 1 mL/50 mL medium, and incubation temperature of 30 °C. The fermentation period was 5 d under oxygen-limited conditions, and the ethanol yield was 24.2 g/L.

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