Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells of Pichia stipitis NCIM 3498

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Chandrasekhar Gajula ◽  
Anuj Kumar Chandel ◽  
Radhika Konakalla ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Immobilized Cells ◽  
Pichia Stipitis ◽  
Fuel Ethanol ◽  
Enzymatic Hydrolysate ◽  
Groundnut Shell

Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells of Pichia stipitis NCIM 3498

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Chandrasekhar Gajula ◽  
Anuj Kumar Chandel ◽  
Radhika Konakalla ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Reducing Sugars ◽  
Sodium Sulfite ◽  
Immobilized Cells ◽  
Pichia Stipitis ◽  
Yeast Cells ◽  
Submerged Cultivation ◽  
Cultivation Conditions ◽  
Enzymatic Hydrolysate ◽  
Groundnut Shell

Groundnut shell (GS), after separation of pod, is readily available as a potential feedstock for production of fermentable sugars. The substrate was delignified with sodium sulfite. The delignified substrate released 670 mg/g of sugars after enzymatic hydrolysis (50°C, 120 rpm, 50 hrs) using commercial cellulases (Dyadic® Xylanase PLUS, Dyadic Inc. USA). The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. Immobilization of yeast cells on sorghum stalks were confirmed by scanning electron microscopy (SEM). A maximum of ethanol production (17.83 g/L, yield 0.44 g/g and 20.45 g/L, yield 0.47 g/g) was observed with free and immobilized cells of P. stipitis respectively in batch fermentation conditions. Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles.

Production of Fuel Ethanol from Hydrotherm-Pretreated Corn Stover by Pichia stipitis

2013 ◽  
Vol 641-642 ◽  
pp. 943-946
Author(s):  
Qiang Zhang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Substrate Concentration ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Fuel Ethanol ◽  
Hydrothermal Pretreatment ◽  
Raw Material ◽  
Initial Ph ◽  
Simultaneous Saccharification

Corn stover is an abundant raw material for fuel ethanol production. Finding out the appropriate process for ethanol production will be possible to achieve industrilization. Hydrothermal pretreatment (195°C,15min) and simultaneous saccharification and fermentation (SSF) were adopted to produce ethanol from corn stover. The results showed that 86.5% of cellulose were remained in solid cake and most of hemicellulose were solubilized after pretreatment. The highest ethanol concentrationl of 12.12 g/ L was achieved at initial pH of 5.5，temperature 30°C and 130 rpm shaking rate with substrate concentration of 5%(w/v) by Pichia stipitis58376 after 192 h.The corresponding volumetric productivity were 0.065g/Lh .

Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium

2012 ◽  
Vol 160 (3-4) ◽  
pp. 229-235 ◽  
Author(s):  
Yu Shen ◽  
Jin-Song Guo ◽  
You-Peng Chen ◽  
Hai-Dong Zhang ◽  
Xu-Xu Zheng ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Nitrogen Source ◽  
Sweet Potato ◽  
Low Cost ◽  
Fuel Ethanol ◽  
High Gravity

Optimizing Liquid Hot Water pretreatment conditions to enhance sugar recovery from wheat straw for fuel-ethanol production

Fuel ◽  
2008 ◽  
Vol 87 (17-18) ◽  
pp. 3640-3647 ◽  
Author(s):  
J.A. Pérez ◽  
I. Ballesteros ◽  
M. Ballesteros ◽  
F. Sáez ◽  
M.J. Negro ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Fuel Ethanol ◽  
Hot Water ◽  
Sugar Recovery ◽  
Liquid Hot Water Pretreatment ◽  
Liquid Hot Water ◽  
Water Pretreatment ◽  
Pretreatment Conditions ◽  
Hot Water Pretreatment

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Full-scale On-farm Pretreatment of Perennial Grasses with Dilute Acid for Fuel Ethanol Production

2010 ◽  
Vol 3 (4) ◽  
pp. 335-341 ◽  
Author(s):  
Matthew Francis Digman ◽  
Kevin J. Shinners ◽  
Richard E. Muck ◽  
Bruce S. Dien
Keyword(s):  
Ethanol Production ◽  
Fuel Ethanol ◽  
Full Scale ◽  
Perennial Grasses ◽  
Dilute Acid ◽  
On Farm
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