Microfauna community during pulp and paper wastewater treatment in a UNOX system

2017 ◽  
Vol 58 ◽  
pp. 143-151 ◽  
Author(s):  
Katarzyna Bernat ◽  
Dorota Kulikowska ◽  
Adam Drzewicki
Keyword(s):  
Wastewater Treatment ◽  
Pulp And Paper ◽  
Paper Wastewater

scholarly journals Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei
Keyword(s):  
Wastewater Treatment ◽  
Xylanase Activity ◽  
Pulp And Paper ◽  
Optimal Conditions ◽  
Paper Wastewater ◽  
Termite Hindgut ◽  
Uncultured Microorganisms ◽  
Metagenomic Approach ◽  
Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

Performance evaluation of anaerobic baffled reactor (ABR) treating pulp and paper wastewater in start-up period

2015 ◽  
Vol 10 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Abolghasem Alighardashi ◽  
Meghdad Modanlou ◽  
Shervin Jamshidi
Keyword(s):  
Wastewater Treatment ◽  
Chemical Oxygen Demand ◽  
Volatile Fatty Acids ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Pulp And Paper ◽  
Anaerobic Baffled Reactor ◽  
Start Up ◽  
Paper Wastewater

This essay outlines the use of an anaerobic baffled reactor (ABR) treating pulp and paper wastewater during its start-up period. For this purpose, a pilot with four chambers and overall volume of 45 liters was fed continuously through the equalization tank of Mazandaran wood and paper wastewater treatment plant, in the north of Iran. The influent was classified as low strength slowly biodegradable wastewater. The average soluble chemical oxygen demand (SCOD) and biochemical oxygen demand (BOD) of the influent were about 1,130 and 320 mg/L, respectively. Results show that the start-up was accomplished in 90 days in which the ABR reached its maximum SCOD removal of 60%. This was achieved at the controlled mesophilic temperature (37 °C) and optimum hydraulic retention time (HRT) of 24 hours. In spite of the influent characteristics, the performance of ABR has not been inhibited and mostly influenced by HRT. The gradual hydrolysis and acidogenesis were observed within the ABR. The majority of chemical oxygen demand (COD) removal takes place in the first chamber. In addition, the concentrations of readily biodegradable organics (BOD to COD ratio) have been increased and doubled through the reactor. Moreover, the total values of pH, volatile fatty acids and alkalinity remained constant. Consequently, this system can be approved for application as a pretreatment unit for paper mill industrial wastewater treatment plants.

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