A controlled antibiotic release system to prevent orthopedic-implant associated infections: An in vitro study

ABSTRACTIncreased functions of osteoblasts (bone-forming cells) have been demonstrated on nanophase compared to conventional ceramics (specifically, alumina, titania, and hydroxyapatite), polymers (such as poly-lactic-glycolic acid and polyurethane), carbon nanofibers, and composites thereof. Nanophase materials are materials that simulate dimensions of constituent components of bone since they possess particle or grain sizes less than 100 nm. However, to date, interactions of osteoblasts on nanophase compared to conventional metals remain to be elucidated. For this reason, the objective of the present in vitro study was to design, fabricate, and evaluate osteoblast adhesion on nanophase metals (specifically, Ti and Ti6Al4V). Results of this study provided the first evidence of increased osteoblast adhesion on nanophase compared to conventional Ti-based metals. Moreover, directed osteoblast adhesion was observed preferentially at metal particle boundaries. It is speculated that since more particle boundaries were created through the use of nanophase compared to conventional metals, increased osteoblast adhesion resulted. Because adhesion is a necessary prerequisite for subsequent functions of osteoblasts (such as deposition of calcium-containing mineral), the present study suggests that Ti-based nanophase metals should be further considered for orthopedic implant applications.

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Antibiotic release and antibacterial efficacy in cement spacers and cement beads impregnated with different techniques: In vitro study

Joint Diseases and Related Surgery ◽

10.5606/ehc.2018.59021 ◽

2018 ◽

Vol 29 (2) ◽

pp. 71-78 ◽

Cited By ~ 3

Author(s):

Teoman Atıcı

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Antibacterial Efficacy ◽

Antibiotic Release

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Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Journal of Korean Neurosurgical Society ◽

10.3340/jkns.2019.0161 ◽

2020 ◽

Vol 63 (1) ◽

pp. 45-55 ◽

Cited By ~ 1

Author(s):

Dong-Won Shin ◽

Moon-Jun Sohn ◽

Chong-Rae Cho ◽

Hae-won Koo ◽

Sang Won Yoon

Keyword(s):

Antibacterial Activity ◽

Fibrin Sealant ◽

In Vitro Study ◽

Vitro Study ◽

Antibiotic Release

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Drug Delivery Assessment of a Novel Triple Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold: An in-vitro Study.

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200605110250 ◽

2020 ◽

Vol 21 ◽

Cited By ~ 1

Author(s):

Azade Rafiee ◽

Mahtab Memarpour ◽

Sara Taghvamanesh ◽

Forough Karami ◽

Somayeh Karami ◽

...

Keyword(s):

High Performance ◽

In Vitro Study ◽

Vitro Study ◽

Burst Release ◽

Platelet Rich Fibrin ◽

Fibrin Scaffold ◽

Antibiotic Release ◽

Simultaneous Identification

Background: Intracanal disinfection is a critical, yet challenging goal for the long-term success in regenerative-based treatments. This in-vitro study aimed to assess the release profile of triple antibiotic-eluting injectable platelet-rich fibrin (I-PRF) constructs in 28 days. Methods: I-PRF scaffolds containing triple antibiotic mixture [metronidazole (MET), ciprofloxacin (CIP), and minocycline (MINO)] by immersion (group one), I-PRF scaffolds containing triple antibiotic mixture by integration (group two), and antibiotic-free I-PRF scaffolds (group three) were fabricated. The antibiotic release from the scaffolds was measured using a high performance liquid chromatography (HPLC) (the mobile phase of 0.1% formic acid and methanol (35:65 v/v), a C18 analytical column (150 × 4.6 mm, 5 μm) at a flow rate of 0.7 mL/min, at 25ºC) at days 1, 3, 7, 14, 21, and 28. Results: Retention times for MINO, CIP, and MET were achieved as 2.3, 2.6, and 3.1 min, respectively. The maximum UV absorbances for CIP, MET, and MINO were at 268 nm, 278 nm, and 350 nm, respectively. The results of the first group showed burst release within the first 24 hours followed by sustained maintenance of all three antibiotics up to 14 days. MINO and MET were still detectable in the third week. The second group could not sustainably release of the antibiotics. Conclusions: The developed method for the simultaneous identification, and quantification of each antibiotic in I-PRF was sensitive and quick. Overall, group one could take up the antibiotics in adequate quantities and then subsequently release them over the study period.

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