Pronounced therapeutic potential of oligonucleotides fixed on inorganic nanoparticles against highly pathogenic H5N1 influenza A virus in vivo

2021 ◽  
Vol 162 ◽  
pp. 92-98
Author(s):  
Asya Levina ◽  
Marina Repkova ◽  
Nadezhda Shikina ◽  
Zinfer Ismagilov ◽  
Maxim Kupryushkin ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Therapeutic Potential ◽  
Inorganic Nanoparticles ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

scholarly journals Differential Contribution of PB1-F2 to the Virulence of Highly Pathogenic H5N1 Influenza A Virus in Mammalian and Avian Species

2011 ◽  
Vol 7 (8) ◽  
pp. e1002186 ◽  
Author(s):  
Mirco Schmolke ◽  
Balaji Manicassamy ◽  
Lindomar Pena ◽  
Troy Sutton ◽  
Rong Hai ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Avian Species ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1 ◽  
Differential Contribution

scholarly journals Novel Approach to the Development of Effective H5N1 Influenza A Virus Vaccines: Use of M2 Cytoplasmic Tail Mutants

2007 ◽  
Vol 82 (5) ◽  
pp. 2486-2492 ◽  
Author(s):  
Tokiko Watanabe ◽  
Shinji Watanabe ◽  
Jin Hyun Kim ◽  
Masato Hatta ◽  
Yoshihiro Kawaoka
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Cytoplasmic Tail ◽  
Influenza Viruses ◽  
Wild Type Virus ◽  
Highly Pathogenic ◽  
Live Attenuated Vaccines ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

ABSTRACT Outbreaks of highly pathogenic H5N1 influenza viruses in avian species began in Asia and have since spread to other continents. Concern regarding the pandemic potential of these viruses in humans is clearly warranted, and there is an urgent need to develop effective vaccines against them. Previously, we and others demonstrated that deletions of the M2 cytoplasmic tail caused a growth defect in A/WSN/33 (H1N1) influenza A virus in vitro (K. Iwatsuki-Horimoto, T. Horimoto, T. Noda, M. Kiso, J. Maeda, S. Watanabe, Y. Muramoto, K. Fujii, and Y. Kawaoka, J. Virol. 80:5233-5240, 2006; M. F. McCown and A. Pekosz, J. Virol. 79:3595-3605, 2005; M. F. McCown and A. Pekosz, J. Virol. 80:8178-8189, 2006). We therefore tested the feasibility of using M2 tail mutants as live attenuated vaccines against H5N1 virus. First we generated a series of highly pathogenic H5N1 (A/Vietnam/1203/04 [VN1203]) M2 cytoplasmic tail deletion mutants and examined their growth properties in vitro and in vivo. We found that one mutant, which contains an 11-amino-acid deletion from the C terminus (M2del11 virus), grew as well as the wild-type virus but replicated in mice less efficiently. We then generated a recombinant VN1203M2del11 virus whose hemagglutinin (HA) gene was modified by replacing sequences at the cleavage site with those of an avirulent type of HA (M2del11-HAavir virus). This M2del11-HAavir virus protected mice against challenge with lethal doses of homologous (VN1203; clade 1) and antigenically distinct heterologous (A/Indonesia/7/2005; clade 2) H5N1 viruses. Our results suggest that M2 cytoplasmic tail mutants have potential as live attenuated vaccines against H5N1 influenza viruses.

scholarly journals Effect of an Asparagine-to-Serine Mutation at Position 294 in Neuraminidase on the Pathogenicity of Highly Pathogenic H5N1 Influenza A Virus

2011 ◽  
Vol 85 (10) ◽  
pp. 4667-4672 ◽  
Author(s):  
M. Kiso ◽  
M. Ozawa ◽  
M. t. Q. Le ◽  
H. Imai ◽  
K. Takahashi ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

scholarly journals The highly pathogenic H5N1 influenza A virus down-regulated several cellular MicroRNAs which target viral genome

2017 ◽  
Vol 21 (11) ◽  
pp. 3076-3086 ◽  
Author(s):  
Rong Wang ◽  
Ying-Ying Zhang ◽  
Jian-Sheng Lu ◽  
Bing-Hui Xia ◽  
Zhi-Xin Yang ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Viral Genome ◽  
Influenza A ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

scholarly journals Glycyrrhizin inhibits highly pathogenic H5N1 influenza A virus-induced pro-inflammatory cytokine and chemokine expression in human macrophages

2010 ◽  
Vol 199 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Martin Michaelis ◽  
Janina Geiler ◽  
Patrizia Naczk ◽  
Patchima Sithisarn ◽  
Henry Ogbomo ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Inflammatory Cytokine ◽  
Highly Pathogenic ◽  
Human Macrophages ◽  
Chemokine Expression ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

scholarly journals Characterization of a Highly Pathogenic H5N1 Avian Influenza A Virus Isolated from Duck Meat

2002 ◽  
Vol 76 (12) ◽  
pp. 6344-6355 ◽  
Author(s):  
Terrence M. Tumpey ◽  
David L. Suarez ◽  
Laura E. L. Perkins ◽  
Dennis A. Senne ◽  
Jae-gil Lee ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Hong Kong ◽  
Influenza A Virus ◽  
Influenza A ◽  
Infectious Virus ◽  
Influenza Viruses ◽  
H5n1 Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Pathogenic H5n1

ABSTRACT Since the 1997 H5N1 influenza virus outbreak in humans and poultry in Hong Kong, the emergence of closely related viruses in poultry has raised concerns that additional zoonotic transmissions of influenza viruses from poultry to humans may occur. In May 2001, an avian H5N1 influenza A virus was isolated from duck meat that had been imported to South Korea from China. Phylogenetic analysis of the hemagglutinin (HA) gene of A/Duck/Anyang/AVL-1/01 showed that the virus clustered with the H5 Goose/Guandong/1/96 lineage and 1997 Hong Kong human isolates and possessed an HA cleavage site sequence identical to these isolates. Following intravenous or intranasal inoculation, this virus was highly pathogenic and replicated to high titers in chickens. The pathogenesis of DK/Anyang/AVL-1/01 virus in Pekin ducks was further characterized and compared with a recent H5N1 isolate, A/Chicken/Hong Kong/317.5/01, and an H5N1 1997 chicken isolate, A/Chicken/Hong Kong/220/97. Although no clinical signs of disease were observed in H5N1 virus-inoculated ducks, infectious virus could be detected in lung tissue, cloacal, and oropharyngeal swabs. The DK/Anyang/AVL-1/01 virus was unique among the H5N1 isolates in that infectious virus and viral antigen could also be detected in muscle and brain tissue of ducks. The pathogenesis of DK/Anyang/AVL-1/01 virus was characterized in BALB/c mice and compared with the other H5N1 isolates. All viruses replicated in mice, but in contrast to the highly lethal CK/HK/220/97 virus, DK/Anyang/AVL-1/01 and CK/HK/317.5/01 viruses remained localized to the respiratory tract. DK/Anyang/AVL-1/01 virus caused weight loss and resulted in 22 to 33% mortality, whereas CK/HK/317.5/01-infected mice exhibited no morbidity or mortality. The isolation of a highly pathogenic H5N1 influenza virus from poultry indicates that such viruses are still circulating in China and may present a risk for transmission of the virus to humans.

Complete genome analysis of a highly pathogenic H5N1 influenza A virus isolated from a tiger in China

2008 ◽  
Vol 153 (8) ◽  
pp. 1569-1574 ◽  
Author(s):  
Muhammad Hassan Mushtaq ◽  
Huang Juan ◽  
Ping Jiang ◽  
Yufeng Li ◽  
TianXian Li ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Genome Analysis ◽  
Complete Genome ◽  
Influenza A ◽  
Highly Pathogenic ◽  
H5n1 Influenza ◽  
Complete Genome Analysis ◽  
Pathogenic H5n1
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