Use of Nanotechnology to Mitigate Biofouling in Stainless Steel Devices Used in Food Processing, Healthcare, and Marine Environments

In humid environments, the formation of biofilms and microfouling are known to be the detrimental processes that first occur on stainless steel surfaces. This is known as biofouling. Subsequently, the conditions created by metabolites and the activity of organisms trigger corrosion of the metal and accelerate corrosion locally, causing a deterioration in, and alterations to, the performance of devices made of stainless steel. The microorganisms which thus affect stainless steel are mainly algae and bacteria. Within the macroorganisms that then damage the steel, mollusks and crustaceans are the most commonly observed. The aim of this review was to identify the mechanisms involved in biofouling on stainless steel and to evaluate the research done on preventing or mitigating this problem using nanotechnology in humid environments in three areas of human activity: food manufacturing, the implantation of medical devices, and infrastructure in marine settings. Of these protective processes that modify the steel surfaces, three approaches were examined: the use of inorganic nanoparticles; the use of polymeric coatings; and, finally, the generation of nanotextures.

Novel In-Situ Gelled Acid System Based on Inorganic Nanoparticles

Gelled acid systems based upon gelation of hydrochloric acid (HCl) are widely used in in both matrix acidizing and fracture acidizing treatments to prevent acidizing fluid leak-off into high permeable zones of a reservoir. The gelled up fluid system helps retard the acid reaction to allow deeper acid penetration for hydrocarbon productivity enhancement. The in-situ gelation is typically achieved by using crosslinked polymers with the acid. Conventional in-situ crosslinked gelled acid systems are made up of polyacrylamide gelling agent, iron based crosslinker and a breaker chemical in addition to other additives, with the acid as the base fluid. However, the polymer-based systems can lead to damaging the formation due to a variety of reasons including unbroken polymer residue. Additionally, the iron-based crosslinker systems can lead to scaling, precipitation and or sludge formation after the acid reacts with the formation, resulting in formation damage and lowering of hydrocarbon productivity. In this paper we showcase a new nanoparticles based gelled acid system that overcomes the inherent challenges faced by conventional in-situ crosslinked gelled acid systems. The new system can work in 5 to 20 % HCl up to 300°F. The new system does not contain any polymer or iron based crosslinker that can potentially damage the formation. It comprises nanoparticles, a gelation activator, acidizing treatment additives along with HCl. The new in-situ gelled acid system has low viscosity at surface making it easy to pump. It gels up at elevated temperatures and pH of 1 to 4, which helps with diverting the tail end acid to tighter or damaged zones of the formation. We demonstrate that the viscosification and eventual gelation of the new system can be achieved as the acid reacts with a carbonate formation and the pH rises above 1. As the acid further reacts and continues to spend there by increasing the pH beyond 4, the gel demonstrates reduction of viscosity. This assists in a better cleanup post the acidizing treatment. Various experimental techniques were used to showcase the development of the nanoparticle based acid diversion fluid. Static and dynamic gelation studies as a function of time, temperature and pH are reported. The gelation performance of the new system was evaluated at temperatures up to 300°F and discussed in the paper. Comparative performance of different types of gelation activators on the gelation profile of the nanoparticles is evaluated. It is also shown that the gelation and viscosity reduction is entirely a pH dependent phenomenon and does not require any additional breaker chemistry, and therefore provides more control over the system performance. The novelty of the new gelled acid system is that it is based upon nanoparticles making it less prone to formation damage as compared to a crosslinked polymer based system.

Protein nanoparticles directed cancer imaging and therapy

Cancer has been a serious threat to human health. Among drug delivery carriers, protein nanoparticles are unique because of their mild and environmentally friendly preparation methods. They also inherit desired characteristics from natural proteins, such as biocompatibility and biodegradability. Therefore, they have solved some problems inherent to inorganic nanocarriers such as poor biocompatibility. Also, the surface groups and cavity of protein nanoparticles allow for easy surface modification and drug loading. Besides, protein nanoparticles can be combined with inorganic nanoparticles or contrast agents to form multifunctional theranostic platforms. This review introduces representative protein nanoparticles applicable in cancer theranostics, including virus-like particles, albumin nanoparticles, silk protein nanoparticles, and ferritin nanoparticles. It also describes the common methods for preparing them. It then critically analyzes the use of a variety of protein nanoparticles in improved cancer imaging and therapy.

Genome-wide DNA methylome and transcriptome changes induced by inorganic nanoparticles in human kidney cells after chronic exposure

The unique physicochemical properties make inorganic nanoparticles (INPs) an exciting tool in diagnosis and disease management. However, as INPs are relatively difficult to fully degrade and excrete, their unintended accumulation in the tissue might result in adverse health effects. Herein, we provide a methylome–transcriptome framework for chronic effects of INPs, commonly used in biomedical applications, in human kidney TH-1 cells. Renal clearance is one of the most important routes of nanoparticle excretion; therefore, a detailed evaluation of nanoparticle-mediated nephrotoxicity is an important task. Integrated analysis of methylome and transcriptome changes induced by INPs (PEG-AuNPs, Fe3O4NPs, SiO2NPs, and TiO2NPs) revealed significantly deregulated genes with functional classification in immune response, DNA damage, and cancer-related pathways. Although most deregulated genes were unique to individual INPs, a relatively high proportion of them encoded the transcription factors. Interestingly, FOS hypermethylation inversely correlating with gene expression was associated with all INPs exposures. Our study emphasizes the need for a more comprehensive investigation of INPs’ biological safety, especially after chronic exposure. Graphical abstract

Green Synthesis of Semiconductors and Environmental Applications

This chapter focuses on advancements in the green synthesis approach for nanomaterials and their environmental applications. The eco-friendly, cost-effective, and simple synthesis of inorganic nanoparticles on the environmental applications are discussed. The first section presents an introduction with the basic topics of the green materials synthesis. The second section summarizes the green chemical methods through different routes (precipitation synthesis, electrospinning synthesis, reflux condensation synthesis, hydrothermal, microwave-assisted hydrothermal methods) and the green routes using biological systems from biosynthetic process to synthesize nanoparticles and biosynthesis of passivating nanofilms using spontaneous formation. In the third section, the environmental and energy applications of nanomaterials, including solar cells, photocatalysis, and biohydrogen production are discussed. Therefore, the scientific community can experience the relation of the achieved nanomaterials with their plentiful application possibilities onto the environmental remediation.

Biodegradation of Alprazolam in Pharmaceutical Wastewater Using Mesoporous Nanoparticles-Adhered Pseudomonas stutzeri

The release of pharmaceutical wastewaters in the environment is of great concern due to the presence of persistent organic pollutants with toxic effects on environment and human health. Treatment of these wastewaters with microorganisms has gained increasing attention, as they can efficiently biodegrade and remove contaminants from the aqueous environments. In this respect, bacterial immobilization with inorganic nanoparticles provides a number of advantages, in terms of ease of processing, increased concentration of the pollutant in proximity of the cell surface, and long-term reusability. In the present study, MCM-41 mesoporous silica nanoparticles (MSN) were immobilized on a selected bacterial strain to remove alprazolam, a persistent pharmaceutical compound, from contaminated water. First, biodegrading microorganisms were collected from pharmaceutical wastewater, and Pseudomonas stutzeri was isolated as a bacterial strain showing high ability to tolerate and consume alprazolam as the only source for carbon and energy. Then, the ability of MSN-adhered Pseudomonas stutzeri bacteria was assessed to biodegrade alprazolam using quantitative HPLC analysis. The results indicated that after 20 days in optimum conditions, MSN-adhered bacterial cells achieved 96% biodegradation efficiency in comparison to the 87% biodegradation ability of Pseudomonas stutzeri freely suspended cells. Kinetic study showed that the degradation process obeys a first order reaction. In addition, the kinetic constants for the MSN-adhered bacteria were higher than those of the bacteria alone.

Recent Developments on Using Nanomaterials to Combat Candida albicans

Vaginal candidiasis (VC) is a common disease of women and the main pathogen is Candida albicans (C. albicans). C. albicans infection incidence especially its drug resistance have become a global health threat due to the existence of C. albicans biofilms and the low bioavailability of traditional antifungal drugs. In recent years, nanomaterials have made great progresses in the field of antifungal applications. Some researchers have treated fungal infections with inorganic nanoparticles, represented by silver nanoparticles (AgNPs) with antifungal properties. Liposomes, polymeric nanoparticles, metal-organic frameworks (MOFs), and covalent organic frameworks (COFs) were also used to improve the bioavailability of antifungal drugs. Herein, we briefly introduced the recent developments on using above nanomaterials to combat C. albicans in antifungal applications.

Versatile Strategy for Electrophoretic Deposition of Polyvinylidene Fluoride-Metal Oxide Nanocomposites

Polyvinylidene fluoride (PVDF) is an advanced functional polymer which exhibits excellent chemical and thermal stability, and good mechanical, piezoelectric and ferroelectic properties. This work opens a new strategy for the fabrication of nanocomposites, combining the functional properties of PVDF and advanced inorganic nanomaterials. Electrophoretic deposition (EPD) has been developed for the fabrication of films containing PVDF and nanoparticles of TiO2, MnO2 and NiFe2O4. An important finding was the feasibility of EPD of electrically neutral PVDF and inorganic nanoparticles using caffeic acid (CA) and catechol violet (CV) as co-dispersants. The experiments revealed strong adsorption of CA and CV on PVDF and inorganic nanoparticles, which involved different mechanisms and facilitated particle dispersion, charging and deposition. The analysis of the deposition yield data, chemical structure of the dispersants and the microstructure and composition of the films provided an insight into the adsorption and dispersion mechanisms and the influence of deposition conditions on the deposition rate, film microstructure and composition. PVDF films provided the corrosion protection of stainless steel. Overcoming the limitations of other techniques, this investigation demonstrates a conceptually new approach for the fabrication of PVDF-NiFe2O4 films, which showed superparamagnetic properties. The approach developed in this investigation offers versatile strategies for the EPD of advanced organic-inorganic nanocomposites.

CuZn and ZnO Nanoflowers as Nano-Fungicides against Botrytis cinerea and Sclerotinia sclerotiorum: Phytoprotection, Translocation, and Impact after Foliar Application

Inorganic nanoparticles (INPs) have dynamically emerged in plant protection. The uptake of INPs by plants mostly depends on the size, chemical composition, morphology, and the type of coating on their surface. Herein, hybrid ensembles of glycol-coated bimetallic CuZn and ZnO nanoparticles (NPs) have been solvothermally synthesized in the presence of DEG and PEG, physicochemically characterized, and tested as nano-fungicides. Particularly, nanoflowers (NFs) of CuZn@DEG and ZnO@PEG have been isolated with crystallite sizes 40 and 15 nm, respectively. Organic coating DEG and PEG (23% and 63%, respectively) was found to protect the NFs formation effectively. The CuZn@DEG and ZnO@PEG NFs revealed a growth inhibition of phytopathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum in a dose-dependent manner with CuZn@DEG NFs being more efficient against both fungi with EC50 values of 418 and 311 μg/mL respectively. Lettuce (Lactuca sativa) plants inoculated with S. sclerotiorum were treated with the NFs, and their antifungal effect was evaluated based on a disease index. Plants sprayed with ZnO@PEG NFs showed a relatively higher net photosynthetic (4.70 μmol CO2 m−2s−1) and quantum yield rate (0.72) than with CuZn@DEG NFs (3.00 μmol CO2 m−2s−1 and 0.68). Furthermore, the penetration of Alizarin Red S-labeled NFs in plants was investigated. The translocation from leaves to roots through the stem was evident, while ZnO@PEG NFs were mainly trapped on the leaves. In all cases, no phytotoxicity was observed in the lettuce plants after treatment with the NFs.