Benidipine, an anti-hypertensive drug, inhibits reactive oxygen species production in polymorphonuclear leukocytes and oxidative stress in salt-loaded stroke-prone spontaneously hypertensive rats

We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE) and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day) for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

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Further Blood Pressure Elevation in spontaneously Hypertensive Rats on High Salt Diet involves Reactive Oxygen Species Production in the Brain.

The FASEB Journal ◽

10.1096/fasebj.21.6.a1363-c ◽

2007 ◽

Vol 21 (6) ◽

Author(s):

Yasuaki Koga ◽

Yoshitaka Hirooka ◽

Shu‐ichirou Araki ◽

Masatsugu Nozoe ◽

Takuya Kishi ◽

...

Keyword(s):

Reactive Oxygen Species Production ◽

Spontaneously Hypertensive Rats ◽

Oxygen Species ◽

Hypertensive Rats ◽

High Salt Diet ◽

Blood Pressure Elevation ◽

Salt Diet ◽

Spontaneously Hypertensive ◽

Species Production ◽

The Brain

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1638: Erectile Dysfunction and Oxidative Stress-Media Ted Cavernosal Cell Death in Spontaneously Hypertensive Rats

The Journal of Urology ◽

10.1016/s0022-5347(18)38846-3 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 433-433

Author(s):

Steven Kuntz ◽

Steven White ◽

Michael Alba ◽

Mahadevan Rajasekaran

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Erectile Dysfunction ◽

Spontaneously Hypertensive Rats ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

And Oxidative Stress

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Diabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats

Cardiovascular Diabetology ◽

10.1186/1475-2840-12-152 ◽

2013 ◽

Vol 12 (1) ◽

pp. 152 ◽

Cited By ~ 26

Author(s):

Camila Rosa ◽

Natasha Xavier ◽

Dijon Henrique Campos ◽

Ana Angélica Fernandes ◽

Marcelo Diarcadia Cezar ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Cardiac Remodeling ◽

Spontaneously Hypertensive Rats ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

And Oxidative Stress

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Abstract 1378: NFkb Blockade Attenuate Cytokines And Oxidative Stress In The Paraventricular Nucleus And Decreases Neurohumoral Excitation In Spontaneously Hypertensive Rats

Circulation ◽

10.1161/circ.116.suppl_16.ii_283-a ◽

2007 ◽

Vol 116 (suppl_16) ◽

Author(s):

Nithya Mariappan ◽

Carrie Elks ◽

Masudul Haque ◽

Philip J Ebnezer ◽

Elizabeth McIIwain ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Pressure ◽

Paraventricular Nucleus ◽

Spontaneously Hypertensive Rats ◽

Left Ventricular ◽

Oxidative Stress Markers ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Stress Markers ◽

And Oxidative Stress

The transcriptional factor, nuclear factor kappa B (NFkB) plays an important role in the regulation of cytokines. Among the cytokines, tumor necrosis factor-alpha (TNF) plays an important role in cardiovascular pathophysiology. This study was done to determine whether TNF-α blockade with etanercept (ETN) or NFkB blockade with dithiol pyrolidine thiocarbamate (PDTC) attenuate oxidative stress in the paraventricular nucleus (PVN) and contribute to neurohumoral excitation in spontaneously hypertensive rats. Method: Male 20 week old SHR rats were treated with ETN (1 mg/kg BW, sc) or PDTC (100mg/kg BW, ip) for 5 week period. Left ventricular function was measured at baseline (20 weeks) and at 25 weeks using echocardiography. Blood pressure was measured at weekly intervals throughout the study. At the end of the protocol rats were sacrificed the PVN was microdissected for the measurement of cytokines, oxidative stress markers using real time PCR (fold increase compared to WKY controls) and by immunohistochemistry. Superoxide, total reactive oxygen species and peroxynitrite were measured in the PVN and LV using electron paramagnetic resonance. Plasma norepinephrine and epinephrine an indicator of neurohumoral excitation was measured using HPLC-EC. Results: PVN data are tabulated. SHR animals had increased expression of protein and mRNA for cytokines and oxidative stress markers in the PVN and LV with increased MAP and cardiac hypertrophy when compared to WKY rats. Treatment with ETN and PDTC attenuated these increases with PDTC showing marked effect than ETN on hypertrophy and blood pressure responses. Conclusion: These findings suggest that cytokine activation in the PVN contributes to increased oxidative stress and neurohumoral excitation in hypertension.

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Effect of Treatment with Peptide Extract from Beef Myofibrillar Protein on Oxidative Stress in the Brains of Spontaneously Hypertensive Rats

Foods ◽

10.3390/foods8100455 ◽

2019 ◽

Vol 8 (10) ◽

pp. 455

Author(s):

Lee ◽

Hur

Keyword(s):

Oxidative Stress ◽

Spontaneously Hypertensive Rats ◽

Myofibrillar Protein ◽

Oxygen Species ◽

Hypertensive Rats ◽

Sod Activity ◽

Spontaneously Hypertensive ◽

Hypertension Therapy ◽

Effect Of Treatment ◽

Gpx Activity

This study was conducted to determine the effect of beef peptide extract on oxidative stress in the brains of spontaneously hypertensive rats (SHRs). A 3-kDa peptide extract was obtained from beef myofibrillar protein using alkaline-AK (AK3K). Oxidative stress in SHR brains was measured by assessing malondialdehyde (MDA) and reactive oxygen species (ROS) concentrations and superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activity. The SHR brains treated with the AK3K peptide extract (400 mg/kg body weight, AK3K400) showed a significant decrease in MDA and ROS contents by 0.33 and 23.92 μM, respectively (p < 0.05) compared to the control. The SOD activity for AK3K400 was 61.26%, around 20% higher than the control. Furthermore, the SHRs treated with the AK3K peptide extract showed results similar to those obtained using captopril, a hypertension drug, except for the MDA level. The study demonstrates that the beef peptide extract inhibits the generation of oxidative stress in the SHR brain and could possibly be used for neuronal hypertension therapy.

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