scholarly journals Differential regulation of serotonin-1A receptor-stimulated [35S]GTPγS binding in the dorsal raphe nucleus by citalopram and escitalopram

2008 ◽  
Vol 583 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Dania V. Rossi ◽  
Teresa F. Burke ◽  
Julie G. Hensler
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Differential Regulation ◽  
Raphe Nucleus ◽  
Serotonin 1A Receptor ◽  
Gtpγs Binding ◽  
Dorsal Raphé

Serotonin actions within the prelimbic cortex induce anxiolysis mediated by serotonin 1a receptors

2018 ◽  
Vol 33 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Paula SM Yamashita ◽  
Daiane S Rosa ◽  
Christopher A Lowry ◽  
Helio Zangrossi
Keyword(s):  
Prefrontal Cortex ◽  
Dorsal Raphe Nucleus ◽  
Behavioral Responses ◽  
Anxiolytic Effect ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Serotonin 1A Receptor ◽  
Serotonin 1A Receptors ◽  
Escape Behaviors ◽  
Dorsal Raphé

Background: Serotonin plays an important role in the regulation of anxiety, acting through complex modulatory mechanisms within distinct brain structures. Serotonin can act through complex negative feedback mechanisms controlling the neuronal activity of serotonergic circuits and downstream physiologic and behavioral responses. Administration of serotonin or the serotonin 1A receptor agonist, (±)-8-hydroxy-2-(dipropylamino)tetralin (8-OH-DPAT), into the prefrontal cortex, inhibits anxiety-like responses. The prelimbic area of the prefrontal cortex regulates serotonergic neurons within the dorsal raphe nucleus and is involved in modulating anxiety-like behavioral responses. Aims: This study aimed to investigate the serotonergic role within the prelimbic area on anxiety- and panic-related defensive behavioral responses. Methods: We investigated the effects of serotonin within the prelimbic area on inhibitory avoidance and escape behaviors in the elevated T-maze. We also extended the investigation to serotonin 1A, 2A, and 2C receptors. Results: Intra-prelimbic area injection of serotonin or 8-OH-DPAT induced anxiolytic effects without affecting escape behaviors. Previous administration of the serotonin 1A receptor antagonist, WAY-100635, into the prelimbic area counteracted the anxiolytic effects of serotonin. Neither the serotonin 2A nor the serotonin 2C receptor preferential agonists, (±)-2,5-dimethoxy-4-iodoamphetamine (DOI) and 6-chloro-2-(1-piperazinyl) pyrazine (MK-212), respectively, affected behavioral responses in the elevated T-maze. Conclusion: Facilitation of serotonergic signaling within the prelimbic area of rats induced an anxiolytic effect in the elevated T-maze test, which was mediated by local serotonin 1A receptors. This inhibition of anxiety-like defensive behavioral responses may be mediated by prelimbic area projections to neural systems controlling anxiety, such as the dorsal raphe nucleus or basolateral amygdala.

Serotonin-1A receptor binding and Reward-dependent Activation are associated within the Human Dorsal Raphe Nucleus as revealed by PET-fMRI.

NeuroImage ◽  
2009 ◽  
Vol 47 ◽  
pp. S176 ◽  
Author(s):  
R. Lanzenberger ◽  
A. Hahn ◽  
C. Windischberger ◽  
W. Wadsak ◽  
A. Holik ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Serotonin 1A Receptor ◽  
Dorsal Raphé

Buspirone-induced changes in the serotonergic and non-serotonergic cells in the dorsal raphe nucleus of rats

2010 ◽  
Vol 473 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Ali Jahanshahi ◽  
Lee Wei Lim ◽  
Harry W.M. Steinbusch ◽  
Veerle Visser-Vandewalle ◽  
Yasin Temel
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Induced Changes ◽  
Dorsal Raphé

027 GABAergic Neurons in the Dorsal Raphe Nucleus Are under the Influence of GABAergic Inputs from the Nucleus Pontis Oralis

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A12-A12
Author(s):  
Jianhua Zhang ◽  
Mingchu Xi ◽  
Simon Fung ◽  
Charles Tobin ◽  
Sharon Sampogna ◽  
...  
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Gabaergic Neurons ◽  
Fluorescent Labeling ◽  
Raphe Nucleus ◽  
Gaba A ◽  
Adult Cats ◽  
Immunohistochemical Techniques ◽  
Fluorescence Immunohistochemistry ◽  
Dorsal Raphé

Abstract Introduction Our previous study has shown that there is a direct connection between GABAergic neurons in the nucleus pontis oralis (NPO) and neurons of the dorsal raphe nucleus (DR), providing a morphological basis for the hypothesis that GABAergic inhibitory processes in NPO play an important role in the generation and maintenance of wakefulness as well as active (REM) sleep through the interaction with neurons in the DR. However, the target of such a GABAergic projection from the NPO within the DR is unknown. In the present study, a double-fluorescent labeling technique was employed to examine the target of GABAergic inputs to the DR. Methods Adult cats were deeply anesthetized and perfused transcardially. Subsequently, the brainstem containing the DR was removed, postfixed and cut into 15 μm coronal sections with a Reichert-Jung cryostat. The sections were immunostained with antibodies against GABA-A or GABA-B receptors and GABA following the procedure of double fluorescence immunohistochemistry. Results Under fluorescence microscopy, a large number of neurons were labeled with antibodies against either GABA-A receptor or GABA-B receptor. In addition, neurons labeled with antibody against GABA were observed in the DR. With double fluorescence immunohistochemical techniques, some neurons labeled by anti-GABA antibody were also stained with antibodies against GABA-A or GABA-B receptors. Conclusion The expression of GABA-A or GABA-B receptors by GABAergic neurons in the DR indicates that GABAergic neurons in the DR receive GABAergic inputs. Our previous study has demonstrated that these GABAergic inputs are from the NPO. These data provide a morphological foundation to support our hypothesis that, during wakefulness, NPO GABAergic “Executive” neurons suppress “Second-Order” GABAergic neurons in the DR, which, in turn, activate (disinhibit) serotonergic wake-on neurons in this nucleus. Support (if any) NS092383

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