PPADS, a P2X receptor antagonist, as a novel inhibitor of the reverse mode of the Na+/Ca2+ exchanger in guinea pig airway smooth muscle

Guinea pig airway smooth muscle (ASM) cells were maintained in a primary tissue culture (passages 1–3). Cells were exposed to human recombinant interleukin-1 beta (IL-1 beta; 20–100 pg/ml) or interleukin-6 (IL-6; 1–4 ng/ml) in the presence of indomethacin (1 microgram/ml) for up to 5 days. Proliferation of ASM cells was assessed with two techniques, direct counting of cells with a hemacytometer and [3H]thymidine incorporation corrected for total protein content. Hypertrophy of ASM cells was assessed by [3H]leucine incorporation (evaluation of protein synthesis), determination of total DNA content, DNA content per cell, and protein content per cell. We observed that the exposure of ASM cells to human recombinant IL-1 beta or IL-6, in all studied concentrations, significantly increased the number of cells as well as [3H]thymidine incorporation into ASM cells. We also found that exposure of ASM to these two cytokines increased [3H]leucine incorporation into the ASM cells and increased protein content and DNA content per single cell. These changes were also concentration dependent. We conclude that the two proinflammatory cytokines, IL-1 beta and IL-6, which are present in asthmatic lungs, increased the proliferation of ASM cells (hyperplasia) as well as their overall size and size of their nuclei, as measured by biochemical markers. These findings are compatible with the presence of ASM hypertrophy.

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Effect ofDesmodium adscendens fraction F1 (DAF1) on tone and agonist-induced contractions of guinea pig airway smooth muscle

Phytotherapy Research ◽

10.1002/ptr.2650030304 ◽

1989 ◽

Vol 3 (3) ◽

pp. 85-90 ◽

Cited By ~ 9

Author(s):

Marian E. Addy ◽

John F. Burka

Keyword(s):

Smooth Muscle ◽

Guinea Pig ◽

Airway Smooth Muscle

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Effects of mucosal removal on guinea-pig airway smooth muscle responsiveness

Clinical Science ◽

10.1042/cs0700571 ◽

1986 ◽

Vol 70 (6) ◽

pp. 571-575 ◽

Cited By ~ 32

Author(s):

Christopher Murlas

Keyword(s):

Smooth Muscle ◽

Guinea Pig ◽

Airway Smooth Muscle ◽

Contractile Response ◽

Electrical Field Stimulation ◽

Physiological Salt Solution ◽

Induced Responses ◽

Field Stimulation ◽

Electrical Field ◽

Airway Mucosa

1. The contractile response to histamine, acetylcholine (ACh), KCl or electrical field stimulation (EFS) was examined in paired tracheal rings (one of each being denuded by mucosal rubbing), which were mounted in muscle chambers filled with a continuously aerated physiological salt solution at 37°C. 2. Removal of the respiratory mucosa increased the sensitivity of airway muscle to ACh, histamine and EFS, but not to KCl. The hypersensitivity of denuded rings to histamine and EFS was greater than to ACh. Atropine reduced the histamine hypersensitivity observed. 3. Pretreating intact preparations with indomethacin augmented their responsiveness to EFS, histamine and ACh. 4. Indomethacin augmentation of histamine- and EFS-induced responses was greater in preparations without epithelium. 5. We conclude that the airway mucosa may be associated with a factor that reduces airway smooth muscle responsiveness to stimulation.

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