Identification of plant genes involved on the initial contact between ectomycorrhizal symbionts (Castanea sativa – European chestnut and Pisolithus tinctorius)

2009 ◽  
Vol 45 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Mónica Sebastiana ◽  
Andreia Figueiredo ◽  
Bartolomeu Acioli ◽  
Lisete Sousa ◽  
Fernando Pessoa ◽  
...  
Author(s):  
Enrico Marcolin ◽  
Mario Pividori ◽  
Fernanda Colombari ◽  
Maria Chiara Manetti ◽  
Francesco Pelleri ◽  
...  

2006 ◽  
Vol 226 (1-3) ◽  
pp. 199-207 ◽  
Author(s):  
Cécile Robin ◽  
Olivier Morel ◽  
Anna-Maria Vettraino ◽  
Charikleia Perlerou ◽  
Stephanos Diamandis ◽  
...  

1990 ◽  
Vol 68 (3) ◽  
pp. 579-593 ◽  
Author(s):  
H. B. Massicotte ◽  
R. L. Peterson ◽  
C. A. Ackerley ◽  
L. H. Melville

The ontogeny and ultrastructure of ectomycorrhizae synthesized between Betula alleghaniensis (yellow birch) and Pisolithus tinctorius, a broad host range fungus, were studied to determine the structural modifications in both symbionts during ectomycorrhiza establishment. A number of stages, including initial contact of hyphae with the root surface, early mantle formation, and mature mantle formation, were distinguished. Interactions between hyphae and root hairs were frequent. As a paraepidermal Hartig net developed, root epidermal cells elongated in a radial direction, but wall ingrowths were not formed. Repeated branching of Hartig net hyphae resulted in extensive fine branches and the compartmentalization of hyphal cytoplasm. Nuclei and elongated mitochondria were frequently located in the narrow cytoplasmic compartments, and [Formula: see text] thickenings developed along walls of cortical cells in primary roots.


2017 ◽  
pp. 79-82
Author(s):  
Gabriella Kovács ◽  
László Radócz

The most destructive pathogen for the European chestnut is the blight fungus Cryphonectria parasitica (Murr.) Barr. The spread of the fungus was very fast in Europe within a few decades in the second half of the past century. During the tree-health checking in the chestnut andwalnut plantation in Romania, Hargita county, next to Homoródkarácsonyfalva village, we especially concentraded on the signs of blight disease occurrence. The grove is laying on a western slope, under a pine forest. This favourable geographical space protects it not only from pathogen attacts, but it has a special, mild microclimate for nut and chestnut trees. The European chestnut could be a valuable member of local forests, opening a new perspective under conditions of climate changes.


2014 ◽  
pp. 87-90
Author(s):  
I. Prgomet ◽  
D. Torello Marinoni ◽  
D. Donno ◽  
Z. Prgomet ◽  
I. Mujić ◽  
...  

2008 ◽  
Vol 57 (6) ◽  
pp. 620-625 ◽  
Author(s):  
Bartolomeu Acioli-Santos ◽  
Mónica Sebastiana ◽  
Fernando Pessoa ◽  
Lisete Sousa ◽  
Andreia Figueiredo ◽  
...  

2016 ◽  
Vol 640 ◽  
pp. 36-41 ◽  
Author(s):  
Bruna De Mônaco Lopes ◽  
Ivo Mottin Demiate ◽  
Vivian Cristina Ito ◽  
Cristina Soltovski de Oliveira ◽  
Marco Aurélio da Silva Carvalho Filho ◽  
...  

2021 ◽  
Vol 12 ◽  
Author(s):  
Vera Pavese ◽  
Andrea Moglia ◽  
Elena Corredoira ◽  
Mª Teresa Martínez ◽  
Daniela Torello Marinoni ◽  
...  

CRISPR/Cas9 has emerged as the most important tool for genome engineering due to its simplicity, design flexibility, and high efficiency. This technology makes it possible to induce point mutations in one or some target sequences simultaneously, as well as to introduce new genetic variants by homology-directed recombination. However, this approach remains largely unexplored in forest species. In this study, we reported the first example of CRISPR/Cas9-mediated gene editing in Castanea genus. As a proof of concept, we targeted the gene encoding phytoene desaturase (pds), whose mutation disrupts chlorophyll biosynthesis allowing for the visual assessment of knockout efficiency. Globular and early torpedo-stage somatic embryos of Castanea sativa (European chestnut) were cocultured for 5 days with a CRISPR/Cas9 construct targeting two conserved gene regions of pds and subsequently cultured on a selection medium with kanamycin. After 8 weeks of subculture on selection medium, four kanamycin-resistant embryogenetic lines were isolated. Genotyping of these lines through target Sanger sequencing of amplicons revealed successful gene editing. Cotyledonary somatic embryos were maturated on maltose 3% and cold-stored at 4°C for 2 months. Subsequently, embryos were subjected to the germination process to produce albino plants. This study opens the way to the use of the CRISPR/Cas9 system in European chestnut for biotechnological applications


Author(s):  
T. Adão ◽  
L. Pádua ◽  
T. M. Pinho ◽  
J. Hruška ◽  
A. Sousa ◽  
...  

<p><strong>Abstract.</strong> In the early 1980′s, the European chestnut tree (<i>Castanea sativa, Mill.</i>) assumed an important role in the Portuguese economy. Currently, the Trás-os-Montes region (Northeast of Portugal) concentrates the highest chestnuts production in Portugal, representing the major source of income in the region (€50M-€60M).</p> <p>The recognition of the quality of the Portuguese chestnut varieties has increasing the international demand for both industry and consumer-grade segments. As result, chestnut cultivation intensification has been witnessed, in such a way that widely disseminated monoculture practices are currently increasing environmental disaster risks. Depending on the dynamics of the location of interest, monocultures may lead to desertification and soil degradation even if it encompasses multiple causes and a whole range of consequences or impacts. In Trás-os-Montes, despite the strong increase in the cultivation area, phytosanitary problems, such as the chestnut ink disease (<i>Phytophthora cinnamomi</i>) and the chestnut blight (<i>Cryphonectria parasitica</i>), along with other threats, e.g. chestnut gall wasp (<i>Dryocosmus kuriphilus</i>) and nutritional deficiencies, are responsible for a significant decline of chestnut trees, with a real impact on production. The intensification of inappropriate agricultural practices also favours the onset of phytosanitary problems. Moreover, chestnut trees management and monitoring generally rely on in-field time-consuming and laborious observation campaigns. To mitigate the associated risks, it is crucial to establish an effective management and monitoring process to ensure crop cultivation sustainability, preventing at the same time risks of desertification and land degradation.</p> <p>Therefore, this study presents an automatic method that allows to perform chestnut clusters identification, a key-enabling task towards the achievement of important goals such as production estimation and multi-temporal crop evaluation. The proposed methodology consists in the use of Convolutional Neural Networks (CNNs) to classify and segment the chestnut fruits, considering a small dataset acquired based on digital terrestrial camera.</p>


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