Nodular diagnosis of contrasting recombinant inbred lines of Phaseolus vulgaris in multi-local field tests under Mediterranean climate

Recombinant inbred lines derived from a cross between a leafhopper-susceptible bean (Phaseolus vulgaris L.), Berna Dutch brown and leafhopper-resistant selection EMP 419 were examined for resistance to the leafhopper species Empoasca abae (Harris) and Empoasca kraemeri Ross and Moore in Ontario, Canada and Colombia, South America, respectively. In both Ontario and Colombia there were significant positive correlations of visual injury scores with percent reductions in seed count, seed yield and seed weights. Leafhopper injury symptoms were significantly correlated with degree of stunting in Ontario, while only leaf burn scores were correlated with stunting in Colombia. Nymph counts were significantly and positively correlated with leaf curl scores in Ontario but not in Colombia, despite significant rank correlations of leaf burn scores and leaf curl scores between these locations. In a second experiment comparing the effects of E. fabae nymph infestations on set of 23 resistant and 5 susceptible recombinant inbred lines (RILs), the latter lines were infested by significantly fewer nymphs. Possible resistance mechanisms to E. fabae and E. kraemeri are discussed. Key words: Potato leafhopper, resistance insect, antixenosis, bean (navy), tolerance

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Predicting progeny performance in common bean (Phaseolus vulgaris L.) using molecular marker-based cluster analysis

Genome ◽

10.1139/g03-002 ◽

2003 ◽

Vol 46 (2) ◽

pp. 259-267 ◽

Cited By ~ 2

Author(s):

Aaron D Beattie ◽

Tom E Michaels ◽

K Peter Pauls

Keyword(s):

Cluster Analysis ◽

Molecular Markers ◽

Phaseolus Vulgaris ◽

Common Bean ◽

Recombinant Inbred ◽

Random Amplified Polymorphic Dna ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Phaseolus Vulgaris L ◽

Branch Angle

Recovery of superior individuals from a cross based solely on the phenotypic characteristics of single-plant selections is inefficient because some traits, like yield, have low heritabilities, or because it is difficult to create the correct conditions for selection, as with disease resistance. In contrast, molecular markers are highly heritable and unaffected by environmental conditions. The objective of this study was to investigate the potential of molecular markers to identify superior lines in a breeding population by examining relationships between genetic distances (GDs) and phenotypic data for eight agronomic and architectural traits (branch angle, height, hypocotyl diameter, lodging, maturity, upper pods, pods per plant, and yield) obtained from three locations over a two-year period. From an elite common bean (Phaseolus vulgaris L.) cross, 110 recombinant inbred lines (RILs) and the two parents were screened with 116 random amplified polymorphic DNA (RAPD) markers. Pairwise GD values were calculated between each line and a selected "target" (the parent 'OAC Speedvale') using the Jaccard method and correlated to the trait data. The correlations were low and non-significant for all traits, except for branch angle (r = 0.30), maturity (r = 0.25), and pods per plant (r = 0.35). The lines were also grouped according to their cluster-based GD from the target parent using UPGMA cluster analysis. Trait data of lines within groups were combined and correlated to cluster-based GD. Correlation values were large and significant for all traits. Additionally, one-half of the top 10 yielding lines and nearly one-third of the best phenotypically ranked lines were present within the 13% of lines clustered nearest the target. A selection method using marker-based cluster analysis (MBCA) is suggested to assist phenotypic selection by directing a breeder's attention to a subsample of the population containing a high proportion of superior lines.Key words: genetic distance, recombinant inbred lines, RAPD.

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Diverse sources of resistance to Spodoptera litura (F.) in groundnut

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79s.1.8 ◽

2019 ◽

Vol 79 (01S) ◽

Author(s):

M. A. Saleem ◽

G. K. Naidu ◽

H. L. Nadaf ◽

P. S. Tippannavar

Keyword(s):

Recombinant Inbred ◽

Spodoptera Litura ◽

Hot Spot ◽

Recombinant Inbred Lines ◽

Insect Pest ◽

Inbred Lines ◽

Leaf Damage ◽

Botanical Variety ◽

Resistant Genotypes ◽

Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

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