Over-Expression of Rose RrLAZY1 Negatively Regulates the Branch Angle of Transgenic Arabidopsis Inflorescence

Branch angle is a key shoot architecture trait that strongly influences the ornamental and economic value of garden plants. However, the mechanism underlying the control of branch angle, an important aspect of tree architecture, is far from clear in roses. In the present study, we isolated the RrLAZY1 gene from the stems of Rosa rugosa ‘Zilong wochi’. Sequence analysis showed that the encoded RrLAZY1 protein contained a conserved GΦL (A/T) IGT domain, which belongs to the IGT family. Quantitative real-time PCR (qRT-PCR) analyses revealed that RrLAZY1 was expressed in all tissues and that expression was highest in the stem. The RrLAZY1 protein was localized in the plasma membrane. Based on a yeast two-hybrid assay and bimolecular fluorescence complementation experiments, the RrLAZY1 protein was found to interact with auxin-related proteins RrIAA16. The over-expression of the RrLAZY1 gene displayed a smaller branch angle in transgenic Arabidopsis inflorescence and resulted in changes in the expression level of genes related to auxin polar transport and signal transduction pathways. This study represents the first systematic analysis of the LAZY1 gene family in R. rugosa. The results of this study will provide a theoretical basis for the improvement of rose plant types and molecular breeding and provide valuable information for studying the regulation mechanism of branch angle in other woody plants.

Identification and Molecular Mapping of a Major Quantitative Trait Locus Underlying Branch Angle in Soybean

Soybean branch angle is a critical architectural trait that affects many other traits of agronomic importance associated with the plant’s productivity and grain yield, and is thus a vital consideration in soybean breeding. However, the genetic basis for modulating this important trait in soybean and many other crops remain unknown. Previously, we developed a recombinant inbred line (RIL) population derived from a cross between a domesticated soybean (Glycine max) variety, Williams 82, and a wild soybean (Glycine soja) accession, PI 479752, and observed drastic variation in plant architecture including branch angle among individual RILs. In this study, one of the RILs possessing extremely wide branch angle (WBA) was crossed with an elite soybean cultivar (LD00-3309) possessing narrow branch angle (NBA) to produce an F2 population composed of 147 plants and F2-derived F3 families for inheritance analysis and QTL mapping. We found that branch angle is controlled by a major QTL located on chromosome 19, designated qGmBa1, and that WBA – derived from the wild soybean accession – is dominant over NBA. This locus was also detected as a major one underlying branch angle by QTL mapping using a subset of the soybean nested association mapping (SoyNAM) population composed of 140 RILs, which were derived from a cross between a landrace, PI 437169B, possessing WBA and an elite variety, IA3023, possessing NBA. Molecular markers located in the QTL region defined by both mapping populations can be used for marker-assisted selection of branch angle in soybean breeding.

Numerical study of a fractal-like tree node micromixer based on Murray’s law

This article focuses on the influence of fractal-like tree node (FTN) on the mixing efficiency and pressure drop of the micromixer. The mixing efficiency of FTN micromixers with different branch angle δ = 30°, 60° and 90° are compared at six kinds of Reynolds (Res). We can get that the micromixer with δ = 90° has higher mixing efficiency at any Re. The mixing results of the center FTN and the stagger FTN micromixer show that the center FTN has better mixing effect. The angle of FTN and the number of FTN are the key to improve the mixing efficiency. They are also the key to change the pressure drop in the microchannel. The FTN can slow down the pressure drop and maintain the stable pressure drop between two adjacent FTNs. The way to obtain a more stable pressure range is to increase the distance between two adjacent FTN. This provides a reliable reference for maintaining a stable pressure in the microchannel.

GhD14 Regulates Plant Architecture and Fiber Development in Cotton

Background: Strigolactone (SL) signaling is essential in regulating plant development. DWARF14 (D14), the SL receptor, interacts with the F-box in MORE AXILLARY GROWTH (MAX2) to modulate SL signaling. However, the biological function of D14 protein is still unknown in cotton.Results: Here, we identified GhD14s in Gossypium hirsutum and resolved its function in cotton plant architecture and fiber development. Subcellular location results revealed that the GhD14D protein was localized to both the cytoplasm and nucleus. GUS staining assay showed that GhD14D was mainly expressed in leaf primordium, inflorescence, axillary bud and stem and expression analysis revealed that GhD14A/D was highly expressed in stem, flower and fiber cells at 20 days post-anthesis (DPA). Silencing GhD14A/D gene expression in upland cotton significantly increased branch angle. Meanwhile, the fiber length and the transcripts of secondary cell wall biosynthesis related genes were also reduced after GhD14A/D gene silencing. In addition, overexpression of GhD14D in Atd14 mutant successfully rescued the phenotype of the d14 mutant with much shoot-branching and short plant height.Conclusions: Our findings suggest that the GhD14 gene contributes to shoot branch development and fiber cell development in cotton. This study deepens our understanding of the biological role of SL signaling in cotton and providing guidance for modifying cotton plant architecture and improving fiber development using genetic engineering to help us breed better cotton varieties in the future.

Polymorphisms and gene expression in the almond IGT family are not correlated to variability in growth habit in major commercial almond cultivars

Almond breeding programs aimed at selecting cultivars adapted to intensive orchards have recently focused on the optimization of tree architecture. This multifactorial trait is defined by numerous components controlled by processes such as hormonal responses, gravitropism and light perception. Gravitropism sensing is crucial to control the branch angle and therefore, the tree habit. A gene family, denominated IGT family after a shared conserved domain, has been described as involved in the regulation of branch angle in several species, including rice and Arabidopsis, and even in fruit trees like peach. Here we identified six members of this family in almond: LAZY1, LAZY2, TAC1, DRO1, DRO2, IGT-like. After analyzing their protein sequences in forty-one almond cultivars and wild species, little variability was found, pointing a high degree of conservation in this family. To our knowledge, this is the first effort to analyze the diversity of IGT family proteins in members of the same tree species. Gene expression was analyzed in fourteen cultivars of agronomical interest comprising diverse tree habit phenotypes. Only LAZY1, LAZY2 and TAC1 were expressed in almond shoot tips during the growing season. No relation could be established between the expression profile of these genes and the variability observed in the tree habit. However, some insight has been gained in how LAZY1 and LAZY2 are regulated, identifying the IPA1 almond homologues and other transcription factors involved in hormonal responses as regulators of their expression. Besides, we have found various polymorphisms that could not be discarded as involved in a potential polygenic origin of regulation of architectural phenotypes. Therefore, we have established that neither the expression nor the genetic polymorphism of IGT family genes are correlated to diversity of tree habit in currently commercialized almond cultivars, with other gene families contributing to the variability of these traits.

VARIASI POHON FRAKTAL MENGGUNAKAN L-SYSTEMS

Fractal tree is simply a trunk and a number of branches, each of which looks like the tree itself. The fractal tree can be generated using the IFS and L-Systems methods. In this article, the author develops fractal tree generation using L-Systems with additional variations. The variations given are in thickness, length, and branch angle. This development is expected to produce more diverse fractal tree patterns. In generating a fractal tree using L-Systems, it begins by determining the letters and symbols to be used. Then determine which axioms should be used. Then the production rules are made together with the determination of the parametric L-Systems. And the last is to determine the probability value for the stochastic L-Systems. In the deterministic L-Systems, thickness variations, length variations, and branch angle variations are carried out. In the variation of branch thickness, if the ratio of the thickness of the left branch is greater than that of the right branch, the fractal tree is skewed to the left. Then in the variation of branch length if the ratio of the length of the left branch is smaller than the ratio of the length of the right branch, the length of the left branch is longer than the length of the right branch. Then at the angle of the branching the smaller the 𝜃 that is included causes the branches to be closer together. The use of stochastic L-Systems can produce more diverse fractal tree patterns, even though they use the same production rules and parameter values

Insight into the dynamics of non-Newtonian carboxy methyl cellulose conveying CuO nanoparticles: significance of channel branch angle and pressure drop

Branching channels are commonly emerged in a considerable variety of engineering applications, in which most of the fluids present non Newtonian behavior, such as in chemical processes. It is noted that in the material forming process, when one suspends nanoparticles in a basic non Newtonian fluid, a completely new non Newtonian fluid is formed with different rheological characteristics from the former ones. In our present numerical research, considering the side branches inclined at varying angles, we focus on the fluid flow and heat transfer of the laminar power-law nanofluid in a rectangular branching channel under the influences of generalized Reynolds number. Both the consistency coefficient and power-law index of the non Newtonian nanofluid, different from those of the base fluid, are described by empirical formula, dependent on the nanoparticle quantity. Finite element method is applied in the research. It is found that a smaller branch angle α can cause a larger fluctuation in pressure near the branched region. Furthermore, negative pressures exist both in the main and side branch with some certain inclination angle. Above all, the new extensive results of velocity contours, temperature, concentration contours along with pressure drop of the changing rheological models provide detailed information for studies on non Newtonian nanofluids in many intricate industrial applications.

Branch Angle and Leaflet Shape are Associated with Canopy Coverage in Soybean

Early canopy coverage is a desirable trait that promotes faster ground coverage, resulting in reduced soil evaporation, increased light interception, biomass production and weed suppression, all of which are important determinants of yield in soybean (Glycine max). Variation in traits comprising shoot architecture can influence canopy coverage, canopy light interception, canopy-level photosynthesis, and source-sink partitioning efficiency. However, little is known about the extent of phenotypic diversity of shoot architecture traits and their genetic control in soybean. Thus, we sought to understand the contribution of shoot architecture traits to canopy coverage and to determine the genetic control of these traits. We examined the natural variation for shoot architecture traits in a set of 399 diverse maturity group I soybean (SoyMGI) accessions to identify relationships between traits, and to identify loci that are associated with canopy coverage and shoot architecture traits. Canopy coverage was correlated with branch angle, number of branches, plant height and leaf shape. Using previously collected 50K SNP data on the SoyMGI panel, we identified QTL associated with branch angle, number of branches, branch density, leaf length/width ratio, days to flowering, maturity, plant height, number of nodes and stem termination. In many cases QTL intervals overlapped with previously described genes or QTL. Of particular note, we found QTL associated with branch angle and leaflet shape located on chromosomes 19 and 4, respectively, and these QTL overlapped with QTL associated with canopy coverage, suggesting the importance of branch angle and leaflet shape in determining canopy coverage. Taken together, our results highlight the role individual architecture traits play in canopy coverage and contribute information on their genetic control that could help facilitate future efforts in their genetic manipulation.

Space Planting, Competition, and Productivity of a Seven-Year-Old Clonal Teak Plantation in the East Java Monsoon Forest Area

Tree breeding of teak results in selected clones with high growth. Intensive silviculture is required to support a large-scale clonal teak plantation. Appropriate spacing is one of the methods to increase forest plantation productivity. Research of teak clone spacing was conducted on a seven-year-old clonal teak plantation with randomized completely block design. The treatments tested in this study were four plant spacing distances, namely, 3 m × 3 m, 6 m × 2 m, 8 m × 2 m, and 10 m × 2 m. Results show that spacing had significantly different effects on diameter, height, bole height, branch angle, crown area, crown projections, volume, and competition index. However, the height growth did not exhibit any significant differences. The 10 m × 2 m spacing produced the best diameter growth, crown area, and competition index, but has a low volume per hectare and the lowest height of free branch and branch angle. Meanwhile, the 3 m × 3 m spacing will increase bole height and stand volume per hectare. This result suggests that spacing could improve the growth of teak clone but must be followed by intensification of proper maintenance to reduce branch angle and increase bole height.