Predicting progeny performance in common bean (Phaseolus vulgaris L.) using molecular marker-based cluster analysis

Genome ◽  
2003 ◽  
Vol 46 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Aaron D Beattie ◽  
Tom E Michaels ◽  
K Peter Pauls
Keyword(s):  
Cluster Analysis ◽  
Molecular Markers ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Recombinant Inbred ◽  
Random Amplified Polymorphic Dna ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Phaseolus Vulgaris L ◽  
Branch Angle

Recovery of superior individuals from a cross based solely on the phenotypic characteristics of single-plant selections is inefficient because some traits, like yield, have low heritabilities, or because it is difficult to create the correct conditions for selection, as with disease resistance. In contrast, molecular markers are highly heritable and unaffected by environmental conditions. The objective of this study was to investigate the potential of molecular markers to identify superior lines in a breeding population by examining relationships between genetic distances (GDs) and phenotypic data for eight agronomic and architectural traits (branch angle, height, hypocotyl diameter, lodging, maturity, upper pods, pods per plant, and yield) obtained from three locations over a two-year period. From an elite common bean (Phaseolus vulgaris L.) cross, 110 recombinant inbred lines (RILs) and the two parents were screened with 116 random amplified polymorphic DNA (RAPD) markers. Pairwise GD values were calculated between each line and a selected "target" (the parent 'OAC Speedvale') using the Jaccard method and correlated to the trait data. The correlations were low and non-significant for all traits, except for branch angle (r = 0.30), maturity (r = –0.25), and pods per plant (r = 0.35). The lines were also grouped according to their cluster-based GD from the target parent using UPGMA cluster analysis. Trait data of lines within groups were combined and correlated to cluster-based GD. Correlation values were large and significant for all traits. Additionally, one-half of the top 10 yielding lines and nearly one-third of the best phenotypically ranked lines were present within the 13% of lines clustered nearest the target. A selection method using marker-based cluster analysis (MBCA) is suggested to assist phenotypic selection by directing a breeder's attention to a subsample of the population containing a high proportion of superior lines.Key words: genetic distance, recombinant inbred lines, RAPD.

scholarly journals Genetic diversity of recombinant inbred lines of Phaseolus vulgaris L.

2021 ◽  
Vol 52 (3) ◽  
Author(s):  
Lidiane dos Santos Gomes Oliveira ◽  
Leandro Pin Dalvi ◽  
Lucimara Cruz de Souza ◽  
Josimar Aleixo da Silva ◽  
Tais Cristina Bastos Soares
Keyword(s):  
Genetic Diversity ◽  
Phaseolus Vulgaris ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Phaseolus Vulgaris L

Yield and insect injury in leafhopper (Empoasca fabae Harris and Empoasca kraemeri Ross & Moore) infested dry beans in Ontario and Colombia

2004 ◽  
Vol 84 (3) ◽  
pp. 891-900 ◽  
Author(s):  
J. D. Murray ◽  
T. E. Michaels ◽  
K. P. Pauls ◽  
C. Cardona ◽  
A. W. Schaafsma
Keyword(s):  
Phaseolus Vulgaris ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Resistance Mechanisms ◽  
Inbred Lines ◽  
Potato Leafhopper ◽  
Phaseolus Vulgaris L ◽  
Positive Correlations ◽  
Empoasca Kraemeri ◽  
Leaf Curl

Recombinant inbred lines derived from a cross between a leafhopper-susceptible bean (Phaseolus vulgaris L.), Berna Dutch brown and leafhopper-resistant selection EMP 419 were examined for resistance to the leafhopper species Empoasca abae (Harris) and Empoasca kraemeri Ross and Moore in Ontario, Canada and Colombia, South America, respectively. In both Ontario and Colombia there were significant positive correlations of visual injury scores with percent reductions in seed count, seed yield and seed weights. Leafhopper injury symptoms were significantly correlated with degree of stunting in Ontario, while only leaf burn scores were correlated with stunting in Colombia. Nymph counts were significantly and positively correlated with leaf curl scores in Ontario but not in Colombia, despite significant rank correlations of leaf burn scores and leaf curl scores between these locations. In a second experiment comparing the effects of E. fabae nymph infestations on set of 23 resistant and 5 susceptible recombinant inbred lines (RILs), the latter lines were infested by significantly fewer nymphs. Possible resistance mechanisms to E. fabae and E. kraemeri are discussed. Key words: Potato leafhopper, resistance insect, antixenosis, bean (navy), tolerance

scholarly journals SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from Lamon

2017 ◽  
Vol 53 (No. 4) ◽  
pp. 168-171 ◽  
Author(s):  
F. Carucci ◽  
R. Garramone ◽  
R. Aversano ◽  
D. Carputo
Keyword(s):  
Molecular Weight ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Phaseolus Vulgaris ◽  
Microsatellite Markers ◽  
Common Bean ◽  
Ssr Markers ◽  
Phaseolus Vulgaris L ◽  
Ssr Analysis

In this study, 12 microsatellite markers (SSR) were evaluated for their applicability to protect from frauds and misuse the Italian PGI product “Common bean from Lamon”. SSR analysis generated polymorphic alleles, with an average of 4 alleles per locus and all in the range of molecular weight between 181 and 284 bp. Twenty-nine variety-specific fragments were identified, which might be reasonably adopted for characterization and traceability purposes. Cluster analysis well outlined the relationships between the genotypes studied. Overall, our study underlines the use and usefulness of molecular markers to protect both farmers and consumers from frauds.

scholarly journals Shoot and Root Traits Contribute to Drought Resistance in Recombinant Inbred Lines of MD 23–24 × SEA 5 of Common Bean

2017 ◽  
Vol 8 ◽  
Author(s):  
Jose Polania ◽  
Idupulapati M. Rao ◽  
Cesar Cajiao ◽  
Miguel Grajales ◽  
Mariela Rivera ◽  
...  
Keyword(s):  
Common Bean ◽  
Drought Resistance ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Root Traits ◽  
Inbred Lines

Nodular diagnosis of contrasting recombinant inbred lines of Phaseolus vulgaris in multi-local field tests under Mediterranean climate

2016 ◽  
Vol 73 ◽  
pp. 100-107 ◽  
Author(s):  
Mohamed Lazali ◽  
Samira Brahimi ◽  
Chahinez Merabet ◽  
Mourad Latati ◽  
Chahinez Benadis ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Local Field ◽  
Recombinant Inbred ◽  
Mediterranean Climate ◽  
Recombinant Inbred Lines ◽  
Field Tests ◽  
Inbred Lines

scholarly journals Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers.

Genetics ◽  
1995 ◽  
Vol 140 (2) ◽  
pp. 745-754 ◽  
Author(s):  
J Xiao ◽  
J Li ◽  
L Yuan ◽  
S D Tanksley
Keyword(s):  
Molecular Markers ◽  
Qtl Analysis ◽  
Recombinant Inbred ◽  
Quantitative Traits ◽  
Genetic Basis ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Rflp Markers ◽  
Major Contributor ◽  
Significant Qtls

Abstract A set of 194 F7 lines derived from a subspecific rice cross showing strong F1 heterosis was backcrossed to the two parents. The materials (388 BC1F7 lines, 194 F8 lines, two parents, F1) were phenotyped for 12 quantitative traits. A total of 37 significant QTLs (LOD > or = 2.0) was detected through 141 RFLP markers in the BC1F7 populations. Twenty-seven (73%) quantitative trait loci (QTLs) were detected in only one of the BC1F7 populations. In 82% of these cases, the heterozygotes were superior to the respective homozygotes. The remaining 10 (27%) QTLs were detected in both BC1F7 populations, and the heterozygote had a phenotype falling between those of the two homozygotes and in no instances were the heterozygotes found to be superior to both homozygotes. These results suggest that dominance complementation is the major genetic basis of heterosis in rice. This conclusion was strengthened by the finding that there was no correlation between most traits and overall genome heterozygosity and that there were some recombinant inbred lines in the F8 population having phenotypic values superior to the F1 for all of the traits evaluated--a result not expected if overdominance was a major contributor to heterosis. Digenic epistasis was not evident.

A molecular marker tightly linked to P, a gene required for flower and seedcoat color in common bean (Phaseolus vulgaris L.), contains the Ty3-gypsy retrotransposon Tpv3g

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 728-736 ◽  
Author(s):  
Paula M Erdmann ◽  
Rian K Lee ◽  
Mark J Bassett ◽  
Phillip E McClean
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Dominant Allele ◽  
Phaseolus Vulgaris L ◽  
P Gene ◽  
Recombinant Inbred Population ◽  
The Common ◽  
Gypsy Retrotransposon

In common bean (Phaseolus vulgaris L.), the expression of color in flower and seedcoat tissues requires the dominant allele of the P gene. The fully recessive p allele completely suppresses color expression in these tissues, whereas in specific genetic backgrounds, the pgri allele potentiates a grayish-white seedcoat and pale violet (nearly white) flowers with two violet dots on the banner petals. As a first step to gaining a better understanding of this important gene, we phenotypically scored an F2 population segregating for P and pgri and subsequently screened contrasting bulk DNA samples with oligonucleotide primers to uncover random amplified polymorphic DNA (RAPD) fragments. OU32300, an RAPD marker linked in coupling phase to the dominant allele, mapped 1.3 cM from P. The core 'BAT93' × 'Jalo EEP558' recombinant inbred population was scored, and the marker mapped to linkage group B7. The segregating fragment was cloned, sequenced, and shown to possess significant homology to the Ty3–gypsy class of retrotransposons. We have named the element Tpv3g. It is estimated that about 100 copies of the element are present in the common bean genome. Phylogenetic analysis placed Tpv3g in the class A group of plant retrotransposons.Key words: common bean, molecular markers, Phaseolus vulgaris L., seedcoat color, Ty3–gypsy retrotransposon.

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