Fluid Shear-stress Increases TRPV4-Expression in Human Endothelial Cells in an Experimental in Vitro Model

Nearly all tissues are supported by the lymphatic system for a variety of functions, including the regulation of fluid balance, the removal of particulate matter from the interstitium, as well as the transport of fat from the intestine to the blood, among others. Despite these important functions, very little is known about the particular mechanisms through which the lymphatics fulfill these roles. Lymphedema, a chronic disease characterized by an inability of the lymphatics to maintain tissue homeostasis and estimated to affect over 130 million people worldwide, can result in serious clinical problems for which there are very few beneficial cures or therapies [1]. While fluid stagnation is the primary clinical manifestation of the disease, severe lymphedema is often correlated with tissue remodeling and the gross accumulation of lipid [1]. Given these symptoms, one must consider the breakdown in the lymphatic response to mechanical load (i.e. fluid balance) in order to understand the progression of the disease.

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Endothelial cells in culture: An in vitro model of the wall shear stress gradient effect

Journal of Biomechanics ◽

10.1016/s0021-9290(98)80349-7 ◽

1998 ◽

Vol 31 ◽

pp. 173 ◽

Cited By ~ 1

Author(s):

O. Boutherin Falson ◽

C. Haond ◽

M. Chaslon ◽

M. Moenner ◽

S. Naili ◽

...

Keyword(s):

Endothelial Cells ◽

Shear Stress ◽

Wall Shear Stress ◽

In Vitro Model ◽

Stress Gradient ◽

Gradient Effect ◽

Cells In Culture ◽

Wall Shear Stress Gradient ◽

Vitro Model

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Autocrine Control of Angiogenesis by Endogenous Acetylcholine in an In Vitro Model Using Human Endothelial Cells

Journal of Cardiovascular Pharmacology ◽

10.1097/fjc.0000000000000221 ◽

2015 ◽

Vol 65 (5) ◽

pp. 508-515 ◽

Cited By ~ 7

Author(s):

Stefan Dhein ◽

Alice Wermke ◽

Sandy von Salisch ◽

Franziska Schlegel ◽

Holger Stepan ◽

...

Keyword(s):

Endothelial Cells ◽

In Vitro Model ◽

Human Endothelial Cells ◽

Autocrine Control ◽

Vitro Model

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Fluid shear stress modulates endothelial inflammation by targeting LIMS2

Experimental Biology and Medicine ◽

10.1177/1535370220943837 ◽

2020 ◽

Vol 245 (18) ◽

pp. 1656-1663

Author(s):

Junyao Wang ◽

Shiyanjin Zhang

Keyword(s):

Shear Stress ◽

Fluid Shear Stress ◽

Inflammatory Factors ◽

Zinc Finger Domain ◽

Human Endothelial Cells ◽

Fluid Shear ◽

Endothelial Inflammation

Mechanosensitive genes regulate multiple cardiovascular pathophysiological processes and disorders; however, the role of flow-sensitive genes in atherosclerosis is still unknown. In this study, we identify LIM Zinc Finger Domain Containing 2 (LIMS2) that acts as a mechanosensitive gene downregulated by disturbed flow (d-flow) both in human endothelial cells (ECs) in vitro and in mice in vivo. Mechanistically, d-flow suppresses LIMS2 expression, which leads to endothelial inflammation by upregulating typical inflammatory factors, VCAM-1, and ICAM-1 in human ECs. The findings indicate that LIMS2, the new flow-sensitive gene, may help us to find a new insight to explain how d-flow caused endothelial inflammation and provide a new therapeutic approach for atherosclerosis in the future.

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