Facile green synthesis and characterization of Gloriosa superba L. tuber extract-capped silver nanoparticles (GST-AgNPs) and its potential antibacterial and anticancer activities against A549 human cancer cells

2021 ◽  
pp. 100460
Author(s):  
Arul Kumar Murugesan ◽  
Balashanmugam Pannerselvam ◽  
Javee Anand ◽  
Rajenderan Murugan ◽  
Devasena Thiyagarajan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Cancer Cells ◽  
Human Cancer ◽  
Synthesis And Characterization ◽  
Anticancer Activities ◽  
Gloriosa Superba ◽  
Human Cancer Cells ◽  
Tuber Extract

Synthesis and characterization of novel ceramide analogs for induction of apoptosis in human cancer cells

2002 ◽  
Vol 181 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Erhard Bieberich ◽  
Bin Hu ◽  
Jeane Silva ◽  
Sarah MacKinnon ◽  
Robert K. Yu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Synthesis And Characterization ◽  
Human Cancer Cells ◽  
Induction Of Apoptosis

Plant-mediated green synthesis and characterization of silver nanoparticles using Phyllanthus emblica fruit extract

2021 ◽  
Author(s):  
Sajib Aninda Dhar ◽  
Rashedul Alam Chowdhury ◽  
Shaon Das ◽  
Md. Khalid Nahian ◽  
Dipa Islam ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Synthesis And Characterization ◽  
Phyllanthus Emblica ◽  
Fruit Extract

scholarly journals Green synthesis and characterization of silver nanoparticles using Cydonia oblong seed extract

2016 ◽  
Vol 6 (7) ◽  
pp. 1023-1029 ◽  
Author(s):  
Faria Zia ◽  
Nida Ghafoor ◽  
Mudassir Iqbal ◽  
Saliha Mehboob
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Seed Extract ◽  
Synthesis And Characterization

scholarly journals Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an In Vitro Model

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Wasitta Rachakhom ◽  
Ratana Banjerdpongchai
Keyword(s):  
Cell Death ◽  
Cancer Cells ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Autophagic Flux ◽  
Dependent Manner ◽  
Anticancer Activities ◽  
Human Cancer Cells ◽  
Regulated Cell Death

Calomelanone, 2 ′ ,6 ′ -dihydroxy-4,4 ′ -dimethoxydihydrochalcone, possesses anticancer activities. This study was conducted to investigate the cytotoxic effect of calomelanone, a dihydrochalcone analogue, on human cancer cells and its associated mechanisms. The cytotoxic effect of calomelanone was measured by MTT assay. Annexin V-FITC/propidium iodide and DiOC6 staining that employed flow cytometry were used to determine the mode of cell death and reduction of mitochondrial transmembrane potential (MTP), respectively. Caspase activities were measured using specific substrates and colorimetric analysis. The expression levels of Bcl-2 family proteins were determined by immunoblotting. Reactive oxygen species were also measured using 2 ′ ,7 ′ -dihydrodichlorofluorescein diacetate and dihydroethidium (fluorescence dyes). Calomelanone was found to be toxic towards various human cancer cells, including acute promyelocytic HL-60 and monocytic leukemic U937 cells, in a dose-dependent manner at 24 h and human hepatocellular HepG2 cells at 48 h. However, the proliferation of HepG2 cells increased at 24 h. Calomelanone was found to induce apoptosis in HL-60 and U937 at 24 h and HepG2 apoptosis at 48 h via the intrinsic pathway by inducing MTP disruption. This compound also induced caspase-3, caspase-8, and caspase-9 activities. Calomelanone upregulated proapoptotic Bax and Bak and downregulated antiapoptotic Bcl-xL proteins in HepG2 cells. Moreover, signaling was also associated with oxidative stress in HepG2 cells. Calomelanone induced autophagy at 24 h of treatment, which was evidenced by staining with monodansylcadaverine (MDC) to represent autophagic flux. This was associated with a decrease of Akt (survival pathway) and an upregulation of Atg5 (the marker of autophagy). Thus, calomelanone induced apoptosis/regulated cell death in HL-60, U937, and HepG2 cells. However, it also induced autophagy in HepG2 depending on duration, dose, and type of cells. Thus, calomelanone could be used as a potential anticancer agent for cancer treatment. Nevertheless, acute and chronic toxicity should be further investigated in animals before conducting investigations in human patients.

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