Combined effects of polyethylene and organic contaminant on zebrafish (Danio rerio): Accumulation of 9-Nitroanthracene, biomarkers and intestinal microbiota

AbstractEquol is a metabolite of the soya isoflavone (ISO) daidzein that is produced by intestinal microbiota. Equol has greater oestrogenic activity compared with other ISO, and it prevents bone loss in postmenopausal women. Resistant starch (RS), which has a prebiotic activity and is a dietary fibre, was reported to promote equol production. Conversely, the intestinal microbiota is reported to directly regulate bone health by reducing inflammatory cytokine levels and T-lymphocytes in bone. The present study evaluated the combined effects of diet supplemented with ISO and RS on intestinal microbiota, equol production, bone mineral density (BMD) and inflammatory gene expression in the bone marrow of ovariectomised (OVX) mice. Female ddY strain mice, aged 8 weeks, were either sham-operated (Sham, n 7) or OVX. OVX mice were randomly divided into the following four groups (seven per group): OVX control (OVX); OVX fed 0·05 % ISO diet (OVX+ISO); OVX fed 9 % RS diet (OVX+RS); and OVX fed 0·05 % ISO- and 9 % RS diet (OVX+ISO+RS). After 6 weeks, treatment with the combination of ISO and RS increased equol production, prevented the OVX-induced decline in trabecular BMD in the distal femur by modulating the enteric environment and altered OVX-induced inflammation-related gene expression in the bone marrow. However, there were no significant differences in bone parameters between the ISO+RS and ISO-alone groups in OVX mice. Our findings suggest that the combination of ISO and RS might alter intestinal microbiota and immune status in the bone marrow, resulting in attenuated bone resorption in OVX mice.

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Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

Frontiers in Microbiology ◽

10.3389/fmicb.2021.782611 ◽

2021 ◽

Vol 12 ◽

Author(s):

Chun Wang ◽

Zixi Yuan ◽

Yingxue Sun ◽

Xiaolong Yao ◽

Ruixuan Li ◽

...

Keyword(s):

Oxidative Stress ◽

Wastewater Treatment ◽

Microbial Community ◽

Danio Rerio ◽

Textile Wastewater ◽

Combined Effects ◽

Adult Zebrafish ◽

Intestinal Microbial Community ◽

Textile Wastewater Treatment ◽

Effluent Exposure

The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (Danio rerio). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic Lactobacillus, Akkermansia, and Lactococcus in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as Mycoplasma, Stenotrophomonas, and Vibrio, deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.

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Enantioselective bioaccumulation and metabolism of lactofen in zebrafish Danio rerio and combined effects with its metabolites

Chemosphere ◽

10.1016/j.chemosphere.2018.09.052 ◽

2018 ◽

Vol 213 ◽

pp. 443-452 ◽

Cited By ~ 6

Author(s):

Fang Wang ◽

Jing Gao ◽

Li Chen ◽

Zhiqiang Zhou ◽

Donghui Liu ◽

...

Keyword(s):

Danio Rerio ◽

Combined Effects

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Acute Effects of Antimony Exposure in Adult Zebrafish ( Danio Rerio): From an Oxidative Stress and Intestinal Microbiota Perspective

SSRN Electronic Journal ◽

10.2139/ssrn.3961634 ◽

2021 ◽

Author(s):

Chun Wang ◽

Zixi Yuan ◽

Yingxue Sun ◽

Ruixuan Li ◽

Ning Ding ◽

...

Keyword(s):

Oxidative Stress ◽

Intestinal Microbiota ◽

Danio Rerio ◽

Adult Zebrafish ◽

Acute Effects

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The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

Frontiers in Microbiology ◽

10.3389/fmicb.2018.01124 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 13

Author(s):

Yi Sheng ◽

Hui Ren ◽

Samwel M. Limbu ◽

Yuhong Sun ◽

Fang Qiao ◽

...

Keyword(s):

Intestinal Microbiota ◽

Danio Rerio ◽

Lipid Deposition ◽

Genes Expression

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Combined effects of alpha particles and depleted uranium on Zebrafish ( Danio rerio ) embryos

Journal of Radiation Research ◽

10.1093/jrr/rrv104 ◽

2016 ◽

Vol 57 (4) ◽

pp. 343-355 ◽

Cited By ~ 6

Author(s):

Candy Y.P. Ng ◽

Sandrine Pereira ◽

Shuk Han Cheng ◽

Christelle Adam-Guillermin ◽

Jacqueline Garnier-Laplace ◽

...

Keyword(s):

Alpha Particle ◽

Danio Rerio ◽

Depleted Uranium ◽

Alpha Particles ◽

High Dose ◽

Zebrafish Embryos ◽

Combined Effects ◽

Spontaneous Transformation ◽

Particle Dose ◽

Danio Rerio Embryos

Abstract The combined effects of low-dose or high-dose alpha particles and depleted uranium (DU) in Zebrafish ( Danio rerio ) embryos were studied. Three schemes were examined—(i) [I L U L ]: 0.44 mGy alpha-particle dose + 10 µg/l DU exposure, (ii) [I H U H ]: 4.4 mGy alpha-particle dose + 100 µg/l DU exposure and (iii) [I H U L ]: 4.4 mGy alpha-particle dose + 10 µg/l DU exposure—in which Zebrafish embryos were irradiated with alpha particles at 5 h post fertilization (hpf) and/or exposed to uranium at 5–6 hpf. The results were also compared with our previous work, which studied the effects of [I L U H ]: 0.44 mGy alpha-particle dose + 100 µg/l DU exposure. When the Zebrafish embryos developed to 24 hpf, the apoptotic signals in the entire embryos, used as the biological endpoint for this study, were quantified. Our results showed that [I L U L ] and [I H U L ] led to antagonistic effects, whereas [I H U H ] led to an additive effect. The effect found for the previously studied case of [I L U H ] was difficult to define because it was synergistic with reference to the 100 µg/l DU exposure, but it was antagonistic with reference to the 0.44 mGy alpha-particle dose. All the findings regarding the four different schemes showed that the combined effects critically depended on the dose response to each individual stressor. We also qualitatively explained these findings in terms of promotion of early death of cells predisposed to spontaneous transformation by alpha particles, interacting with the delay in cell death resulting from various concentrations of DU exposure.

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