Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production

2021 ◽  
pp. 109976
Author(s):  
Aline F. Oliveira ◽  
Reinaldo G Bastos ◽  
Lucimara G de la Torre
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Alginate ◽  
Lipase Production

Enhancement of Thermostable Lipase Production by a Genotypically Identified Extremophilic Bacillus subtilis NS 8 in a Continuous Bioreactor

2011 ◽  
Vol 20 (2) ◽  
pp. 105-115 ◽  
Author(s):  
Akanbi Taiwo Olusesan ◽  
L. Kamaruzaman Azura ◽  
Fatimah Abubakar ◽  
Abdul Karim Sabo Mohamed ◽  
Son Radu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Lipase Production ◽  
Thermostable Lipase ◽  
Continuous Bioreactor

scholarly journals Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Francisco Fábio Cavalcante Barros ◽  
Ana Paula Resende Simiqueli ◽  
Cristiano José de Andrade ◽  
Gláucia Maria Pastore
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Lipase Production ◽  
Industrial Processes ◽  
Technical Literature ◽  
Wide Range ◽  
Cassava Wastewater ◽  
Agroindustrial Wastes ◽  
Better Than

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.

scholarly journals Lipase Production from Bacillus subtilis using various Agricultural waste

2017 ◽  
Vol 3 (5) ◽  
pp. 405-409
Author(s):  
Naaz Abbas ◽  
Javeria Javed ◽  
Zainab Abbas ◽  
Shahnaz Choudry ◽  
Sakhawat Ali
Keyword(s):  
Bacillus Subtilis ◽  
Agricultural Waste ◽  
Lipase Production

Immobilization of cellulase from newly isolated strain Bacillus subtilis TD6 using calcium alginate as a support material

2011 ◽  
Vol 35 (1-2) ◽  
pp. 29-33 ◽  
Author(s):  
Dian Andriani ◽  
Changshin Sunwoo ◽  
Hwa-Won Ryu ◽  
Bambang Prasetya ◽  
Don-Hee Park
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Alginate ◽  
Support Material

scholarly journals SELECTION, PRODUCTION AND CHARACTERIZATION OF BACILLUS LIPASE

2011 ◽  
Vol 14 (3) ◽  
pp. 64-72
Author(s):  
Khoa Dang Tran ◽  
Huy Quang Le ◽  
Nghiep Dai Ngo
Keyword(s):  
Bacillus Subtilis ◽  
Liquid Culture ◽  
Culture Medium ◽  
Lipase Production ◽  
Liquid Culture Medium ◽  
Optimum Ph ◽  
Active Lipase ◽  
Substrate Concentrations ◽  
Bacillus Strains

The objective of this study is to screen Bacillus strains for high lipase production; from 10 strains of Bacillus subtilis OII (BS7) has ability the best lipase production. The maximum lipase was carried out using liquid culture medium at pH 7,0, substrate concentrations 1,2% (w/w) after 3 days. The optimum pH and temperature on lipase activity were found to be pH 10,0 and 60 oC respectively. Ion Mn2+, Ca2+ are the ions remain activity; active lipase was inhibited by Cu2+ , Zn2 ; solution of SDS 0,2 % decreases 95% activity. Molecular weight of lipase production by BS7 is approximate 23,8kDa. The results are preconditions for the research on recombinant lipase by Bacillus subtilis OII.

scholarly journals Optimization and Immobilization of alpha-amylase from Bacillus subtilis in calcium alginate and calcium alginate – cellulosic residue beads

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Abdallah Herizi ◽  
Souilah Rachid ◽  
Djabali Djaffar ◽  
Nadjemi Boubekeur
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Alginate ◽  
Immobilized Enzymes ◽  
Alginate Beads ◽  
Alpha Amylase ◽  
Inhibitory Effects ◽  
Calcium Alginate Beads ◽  
Factorial Design Of Experiments ◽  
Full Factorial ◽  
Hydrolysis Of

In this study, Alpha amylase from Bacillus subtilis was immobilized by entrapment in Calcium Alginate beads (CA). To improve the properties of these beads, alginate was blended with Cellulosic Residue (CR) obtained from sorghumstarch extraction. The conditions of entrapment were optimized for a maximum immobilization yield (Y%) by mathematical statistics, where the 23-full factorial design of experiments was used. The properties of calcium alginate beads were improved by comparing the activity of immobilized enzymes in the hydrolysis of starch. The activity of the immobilized enzyme by Calcium Alginate /Cellulosic Residue (CA/CR) was found to be higher than the Calcium Alginate method. Zn2+ and Cu2+ have inhibitory effects on both immobilized enzymes. The Bacillus subtilis immobilized in alginate can be reused for 7 cycles with 12.7 μmol of reduced sugars and 6 cycles for the entrapped enzyme in CA/CR with 30 μmol of reduced sugars.

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