PSVI-7 Effect of substrates of agroindustrial wastes fermented with LAB and yeasts on productive, reproductive and health indicators in primiparous guinea pigs

El objetivo de este estudio fue evaluar el posible efecto probiótico del sustrato de residuos agroindustriales fermentado con BAL y levaduras sobre los indicadores productivos, reproductivos y de salud de cobayas primíparas y su descendencia. Se utilizaron 80 cuyes nulíparas distribuidas en 4 grupos de 20 hembras nulíparas cada uno, de 120 ± 5 días de edad, 1450 ± 25 g de peso vivo, todas sometidas al mismo sistema de manejo y alimentación actual de la granja. Se aplicaron con una dosis de 1 mL del bioaditivo / animal. Los tratamientos suministrados fueron las siguientes variantes: T1. control. T2, Sustrato (residuos agroindustriales) fermentado con L. acidophilus ATCC® 4356 ™. T3, sustrato más Kluyveromyces fragilis L4 (UCLV). T4, sustrato fermentado con microorganismos T2 y T3.En el experimento se utilizó un diseño completamente al azar, donde se evaluaron los indicadores reproductivos, productivos y de salud. Weight gain in pregnancy was greater (P < 0.05) in T4 in this same group, weight loss was less (P < 0.05) in lactation; the age at first conception and at delivery decreased (P < 0.05) in T4; fertility, the number of live-born animals and the weight at birth and at weaning were higher (P < 0.05) in T4 compared to the other groups; Likewise, blood parameters notably improved in the animals of treatment 4. The treaties with bioaditive were the ones whit the best behavior as reflected in table 1. In the experiment, the beneficial effect as a probiotic was confirmed by the treatments (T2, T3 and T4) of these with the best performance was T4. The inclusion of substrates fermented with LAB and yeasts significantly improves the productive, reproductive and health indicators of primiparous guinea pigs and their offspring.

Bioconversion of cassava bagasse and sugarcane bagasse using cheap home-made enzymatic cocktails

The agricultural industries generate lignocellulosic wastes that can be modified by fungi to generate high value-added products. The aim of this work was to analyze the efficiency of the bioconversion of sugarcane bagasse and cassava bagasse using two cheap home-made enzymatic cocktails from Aspergillus niger LBM 134 (produced also from agroindustrial wastes) and compare the hydrolysis yield with that obtained from the bioconversion using commercial enzymes. Sugarcane bagasse and cassava bagasse were pretreated with a soft alkaline solution before the hydrolysis carried out with home-made enzymatic cocktails of A. niger LBM 134 and with commercial enzymes to compare their performances. Mono and polysaccharides were analyzed before and after the bioconversion of both bagasses as well as their microscopic structure. The maximal yield was the 80% of total glucans saccharified from cassava bagasse. The bioconversion of both bagasses were better when we used the home-made enzymatic cocktails than commercial enzymes. We obtained high added-value products from agroindustrial wastes, home-made enzymatic cocktails and hydrolysates rich in fermentable sugars. The importance of this work lays in the higher performance of the cheap home-made enzymatic cocktails over the hydrolytic performance of commercial enzymes due to the cost of producing the home-made enzymatic cocktails were more than 500 times lower than commercial enzymes.

Agroindustrial Wastes as a Support for the Immobilization of Lipase from Thermomyces lanuginosus: Synthesis of Hexyl Laurate

As a consequence of intense industrialization in the last few decades, the amount of agro-industrial wastes has increasing, where new forms of valorization are crucial. In this work, five residual biomasses from Maranhão (Brazil) were investigated as supports for immobilization of lipase from Thermomyces lanuginosus (TLL). The new biocatalysts BM-TLL (babaçu mesocarp) and RH-TLL (rice husk) showed immobilization efficiencies >98% and hydrolytic activities of 5.331 U g−1 and 4.608 U g−1, respectively, against 142 U g−1 by Lipozyme® TL IM. High esterification activities were also found, with 141.4 U g−1 and 396.4 U g−1 from BM-TLL and RH-TLL, respectively, against 113.5 U g−1 by TL IM. Results of porosimetry, SEM, and BET demonstrated BM and RH supports are mesoporous materials with large hydrophobic area, allowing a mixture of hydrophobic adsorption and confinement, resulting in hyperactivation of TLL. These biocatalysts were applied in the production of hexyl laurate, where RH-TLL was able to generate 94% conversion in 4 h. Desorption with Triton X-100 and NaCl confirmed that new biocatalysts were more efficient with 5 times less protein than commercial TL IM. All results demonstrated that residual biomass was able to produce robust and stable biocatalysts containing immobilized TLL with better results than commercial preparations.