scholarly journals Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis

2012 ◽  
Vol 130 (3) ◽  
pp. 205-208 ◽  
Author(s):  
Ana Lúcia R. Gonçalves ◽  
Claudio V. Silva ◽  
Marlene T. Ueta ◽  
Julia M. Costa-Cruz
Keyword(s):  
Immune Complex ◽  
Serum Samples ◽  
Strongyloides Venezuelensis ◽  
Complex Detection ◽  
Antigen Antibody

Use of larval, parasitic female and egg antigens fromStrongyloides venezuelensisto detect parasite-specific IgG and immune complexes in immunodiagnosis of human strongyloidiasis

Parasitology ◽  
2012 ◽  
Vol 139 (7) ◽  
pp. 956-961 ◽  
Author(s):  
A. L. R. GONÇALVES ◽  
D. S. NUNES ◽  
M. R. F. GONÇALVES-PIRES ◽  
M. T. UETA ◽  
J. M. COSTA-CRUZ
Keyword(s):  
Immune Complex ◽  
Immune Complexes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Efficiency ◽  
Serum Samples ◽  
Parasitic Female ◽  
Strongyloides Venezuelensis ◽  
Complex Detection ◽  
Specific Igg ◽  
Sensitivity Specificity

SUMMARYThe aim of this study was to use larval, parasitic female and egg antigens fromStrongyloides venezuelensisto detect parasite-specific IgG and immune complexes in human serum samples by enzyme-linked immunosorbent assay (ELISA). In total, 95 serum samples were analysed, consisting of 30 patients harbouringS. stercoralislarvae, 30 healthy subjects and 35 patients with other parasites. Sensitivity, specificity and diagnostic efficiency were calculated. A significant statistical difference was found in the detection of immune complexes and antibodies in patients harbouringS. stercoralislarvae from larval and eggs antigens, with higher positivity using larval antigen. The larval antigen showed the highest values for sensitivity, specificity and diagnostic efficiency in ELISA from detection of immune complexes. For the first time we used IgG anti-larvae, IgG anti-parasitic females or IgG anti-eggs for immune complex detection. We concluded that the association of antibody and immune complex detection could be used in the diagnosis of human strongyloidiasis.

Specific IgG and immune complex responses to parthenogenetic females and eggs of nematode Strongyloides venezuelensis for the diagnosis of immunosuppression in infected rats

2015 ◽  
Vol 90 (3) ◽  
pp. 342-346 ◽  
Author(s):  
A.L.R. Gonçalves ◽  
K.C.L. de Araújo ◽  
E.F.G. Carvalho ◽  
M.T. Ueta ◽  
J.M. Costa-Cruz
Keyword(s):  
Immune Complex ◽  
Immune Complexes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Parthenogenetic Female ◽  
Strongyloides Venezuelensis ◽  
Egg Extracts ◽  
Complex Detection ◽  
Specific Igg ◽  
Bonferroni Test

AbstractIn the present study, antigens from parthenogenetic females and eggs of Strongyloides venezuelensis, or anti-parthenogenetic-female and anti-egg antigens were used to detect specific IgG and immune complex responses, respectively. Serum samples from experimentally infected immunocompetent and immunosuppressed rats were analysed on days 5, 8, 13 and 21 post-infection (dpi). An enzyme-linked immunosorbent assay (ELISA) was performed using alkaline parasite extract for specific IgG detection, and anti-parthenogenetic-female or anti-egg antigens for immune complex detection. The data were analysed using analysis of variance (ANOVA), followed by a Bonferroni test. When parthenogenetic female or egg extracts were used as antigens, specific IgGs were not detected in either immunocompetent or immunosuppressed rats. When anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used, immune complexes were detected for the duration of the infection in immunosuppressed animals and were only detected between 5 and 13 dpi in immunocompetent animals. The duration of infection was not significantly different between the immunocompetent and immunosuppressed groups when anti-parthenogenetic-female or anti-S. venezuelensis-eggs were used. Parthenogenetic female extracts yielded significant differences between antibody and immune complex responses in immunocompetent rats from 5 to 13 dpi, but only on day 5 dpi in immunosuppressed rats. Exposure to S. venezuelensis egg extract yielded significant differences in both antibody and immune complex detection between immunocompetent and immunosuppressed rats for the duration of the infection. In conclusion, ELISA using alternative antigens may be a successful strategy for identifying immune complexes in serum samples and diagnosing active strongyloidiasis, particularly under conditions of immunosuppression.

Immune complexes: characteristics, clinical correlations, and interpretive approaches in the clinical laboratory.

1982 ◽  
Vol 28 (6) ◽  
pp. 1259-1271 ◽  
Author(s):  
S E Ritzmann ◽  
J C Daniels
Keyword(s):  
Immune Complex ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Complex Disease ◽  
Clinical Medicine ◽  
Clinical Laboratory ◽  
Therapeutic Monitoring ◽  
Serum Samples ◽  
Complement Components ◽  
Antigen Antibody

Abstract Immune-complex-mediated injury is thought to play a role in diseases such as rheumatoid arthritis, systemic lupus erythematosus, serum sickness, various infectious diseases, and malignancies. With increased appreciation of the biological and pathological significance of circulating immune complexes has come efforts to develop appropriate techniques for identifying and measuring them. Common approaches exploit such phenomena as the attachment of complement components to antigen-antibody complexes, the presence of specialized receptors for immune complexes at the surface of cells, and the ability of rheumatoid factor to bind with immune complexes. This variety of assay systems for immune complexes has yielded abstruse results in numerous human pathological conditions. Unfortunately, these results seldom correlate with one another in a given disease. Thus, use of a panel of immune complex assays has been recommended. Indirect consequences of immune complex disease may still be appraised and evaluated with some confidence in clinical medicine: measurements of C3 and C4, cryoglobulins, serum viscosity, and turbidity of serum samples. Measurement of immune complexes may be useful in diagnosis, prognosis, and therapeutic monitoring, but it is the characterization of immune complexes that holds the greatest potential for better understanding of disease mechanisms.

Nephrotic Syndrome Associated with Varicella Infection

PEDIATRICS ◽  
1985 ◽  
Vol 75 (6) ◽  
pp. 1127-1131
Author(s):  
Ching-Yuang Lin ◽  
Hey-Chi Hsu ◽  
Han-Yang Hung
Keyword(s):  
Nephrotic Syndrome ◽  
Immune Complex ◽  
Alternative Pathway ◽  
Virus Antigen ◽  
Varicella Infection ◽  
Immune Complex Glomerulonephritis ◽  
Varicella Virus ◽  
Complex Deposition ◽  
Properdin Factor B ◽  
Antigen Antibody

A 4-year-old boy developed nephrotic syndrome following varicella infection. Serologic studies during the early phase of the disease demonstrated a decrease in serum C3, C4, and properdin factor B. Renal biopsy revealed an acute proliferative glomerulonephritis with deposition of immunoglobulins A (IgA) and M, C3, Clq, and varicella virus antigen in the glomerulus, suggesting an immune complex deposition. Ultrastructurally, this suggested a postinfectious immune complex glomerulonephritis. These phenomena suggested that varicella virus antigen antibody complexes were deposited in the glomerulus and activated the classic and alternative pathway of complements, leading to an immune complex glomerulonephritis. During the nephrotic phase, an increase in OKT8 cells and decrease of the OKT4 cells were demonstrated. Two months later, this alteration returned to normal as the renal disease was in remission. This change of lymphocyte subsets during varicella infection may play a role in the pathogenesis of nephrotic syndrome.

PATHOGENESIS OF IMMUNE COMPLEX GLOMERULONEPHRITIS OF NEW ZEALAND MICE

1971 ◽  
Vol 134 (3) ◽  
pp. 65-71 ◽  
Author(s):  
Frank J. Dixon ◽  
Michael B. A. Oldstone ◽  
Giorgio Tonietti
Keyword(s):  
New Zealand ◽  
Immune Complex ◽  
Antibody Formation ◽  
Antinuclear Antibody ◽  
Viral Infections ◽  
Antibody Responses ◽  
Viral Antigens ◽  
Chronic Viral Infections ◽  
Nuclear Antigens ◽  
Antigen Antibody

Observations based on elution of IgG from nephritic kidneys of NZ mice and absorption of the eluted IgG with selected antigens indicate that their immune complex nephritis involves at least two kinds of antigen-antibody complexes. Antibodies reactive with nuclear antigens account for nearly half of the IgG eluted from the kidneys while antibodies reactive with Gross viral antigens make up a significant but lesser amount. Superimposed chronic viral infections affect the nephritis of NZ mice in different ways. LCM and polyoma infections hasten and intensify the antinuclear antibody responses and glomerulonephritis of these mice while LDV infection appears to protect against both antinuclear antibody formation and development of nephritis.

An Apparent Effect Of Size Distribution Of FVIIIR: Ag Multimers On Immunoradiometric Assays

1981 ◽  
Author(s):  
M A Lamb ◽  
H M Reisner ◽  
H A Cooper ◽  
R H Wagner
Keyword(s):  
Complex Formation ◽  
Immune Complex ◽  
Dose Response ◽  
Response Curves ◽  
Crossed Immunoelectrophoresis ◽  
Normal Human ◽  
Dose Response Curves ◽  
Antigen Antibody ◽  
Differential Precipitation ◽  
Immune Complex Formation

Immunoradiometric assays (IRMA) of FVIIIR: Ag from normal and certain variant VWD plasmas have suggested possible antigenic differences in the molecules. Studies reported thus far have used antibody specific for normal FVIIIR: Ag. We have further studied this question of antigenic differences using 2 populations of antibody isolated from an antisera prepared against highly purified human FVIII. Isolated IgG was labeled with [125-I]. The population of labeled IgG “specific” for variant FVIIIR: Ag was separated by immune complex formation with a VWD plasma previously shown, by 2% agarose crossed immunoelectrophoresis, to contain only the lower molecular weight multimers of FVIIIR: Ag. The “specific” labeled IgG was obtained by low pH dissociation and subsequent G-200 chromatography. [125-I] IgG “specific” for normal FVIIIR: Ag was similarly obtained after immune complex formation with pooled normal human plasma. Liquid phase IRMAs were performed using differential precipitation with ammonium sulfate or PEG to separate antigen-antibody complexes from free antibody. Using antibody “specific” for normal FVIIIR: Ag, a lack of parallelism was noted in the dose-response curves of variant plasmas as well as a decrease in maximum antibody bound, as compared to normal. Interestingly, when this antibody was absorbed with the variant VWD plasma and the remaining antibody used in IRMAs, none was bound by either variant or normal plasma.Using antibody “specific” for variant FVIIIR: Ag, a similar lack of parallelism in dose-response curves and a decrease in maximum antibody bound were observed. Therefore rather than antigenic differences as previously implied, these results suggest that the discrepancies noted in IRMAs of variant and normal plasmas are a function of the size of the FVIIIR: Ag multimers.

Dermatitis herpetiformis: immune complex detection with CIq and monoclonal rheumatoid factor

1981 ◽  
Vol 105 (2) ◽  
pp. 159-165 ◽  
Author(s):  
ROBERT E. JORDON ◽  
GERHARD TAPPEINER ◽  
JANE C. KAHL ◽  
KLAUS WOLFF
Keyword(s):  
Rheumatoid Factor ◽  
Immune Complex ◽  
Dermatitis Herpetiformis ◽  
Complex Detection

Qualitative testing for circulating immune complexes by use of zone electrophoresis on agarose.

1980 ◽  
Vol 26 (3) ◽  
pp. 396-402
Author(s):  
R H Kelly ◽  
M A Scholl ◽  
V S Harvey ◽  
A G Devenyi
Keyword(s):  
Immune Complex ◽  
Immune Complexes ◽  
Complex Disease ◽  
Circulating Immune Complexes ◽  
Rectangular Pattern ◽  
Zone Electrophoresis ◽  
Experimental Approaches ◽  
Complex Detection ◽  
Vitro Analysis

Abstract On binding of antibody to antigen an immune complex is formed that has a net surface charge different from that of either of the two components. This, together with clonal restriction of the antibody response, gives rise to distinctive patterns that are readily apparent in stained agarose gels after routine zone electrophoresis. Most circulating immune complexes appear as a rectangular pattern, with well-defined edges, located in the gamma-region. The identity of the material responsible for these patterns has been established by three different experimental approaches: analysis of tetanus/anti-tetanus complexes formed in vitro, analysis of sera from rabbits with experimental immune complex disease, and analysis of human type II and type III cryoglobulins. Studies of reproducibility, interfering substances, and correlation with other assays for detecting immune complexes indicate that zone electrophoresis in agarose gel is a sensitive, highly specific technique for immune complex detection, of potential value as a screening tool.

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