Thymoquinone induces oxidative stress-mediated apoptosis through downregulation of Jak2/STAT3 signaling pathway in human melanoma cells

2021 ◽  
Vol 157 ◽  
pp. 112604
Author(s):  
Pawan Kumar Raut ◽  
Hui Seong Lee ◽  
Sang Hoon Joo ◽  
Kyung-Soo Chun
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Stat3 Signaling ◽  
Human Melanoma Cells ◽  
Stat3 Signaling Pathway

scholarly journals Mechanism of miR-218-5p in autophagy, apoptosis and oxidative stress in rheumatoid arthritis synovial fibroblasts is mediated by KLF9 and JAK/STAT3 pathways

2021 ◽  
pp. jim-2020-001437
Author(s):  
Ming Chen ◽  
Minghui Li ◽  
Na Zhang ◽  
Wenwen Sun ◽  
Hui Wang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Signaling Pathway ◽  
Synovial Tissue ◽  
Synovial Fibroblasts ◽  
Reverse Transcription Pcr ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
Interfering Rna ◽  
And Oxidative Stress

This study was aimed to investigate the effects of miR-218-5p on the proliferation, apoptosis, autophagy, and oxidative stress of rheumatoid arthritis synovial fibroblasts (RASFs), and the related mechanisms. Quantitative reverse transcription–PCR showed that the expression of miR-218-5p in rheumatoid arthritis synovial tissue was significantly higher than that in healthy synovial tissue. Compared with healthy synovial fibroblasts, miR-218-5p expression was obviously upregulated in RASFs, while KLF9 protein expression was markedly downregulated. Mechanistically, miR-218-5p could directly bind to the 3′ untranslated region of KLF9 to inhibit the expression of KLF9. Additionally, transfection of miR-218-5p small interfering RNA (siRNA) inhibited the proliferation but promoted apoptosis and autophagy of RASFs. Simultaneously, miR-218-5p silencing reduced reactive oxygen species and malondialdehyde levels and increased superoxide dismutase and glutathione peroxidase activity to improve oxidative stress in RASFs. More importantly, the introduction of KLF9 siRNA reversed the effects of miR-218-5p siRNA transfection on RASF proliferation, apoptosis, autophagy, and oxidative stress. What is more, silencing miR-218-5p inhibited the activation of JAK2/STAT3 signaling pathway by targeting KLF9. Collectively, knockdown of miR-218-5p could regulate the proliferation, apoptosis, autophagy and oxidative stress of RASFs by increasing the expression of KLF9 and inhibiting the activation of the JAK2/STAT3 signaling pathway, which may provide a potential target for the mechanism research of RA.

scholarly journals NF-YA promotes invasion and angiogenesis by upregulating EZH2-STAT3 signaling in human melanoma cells

2016 ◽  
Vol 35 (6) ◽  
pp. 3630-3638 ◽  
Author(s):  
ZIHAN XU ◽  
YAOWEN SUN ◽  
YADONG GUO ◽  
GAOPING QIN ◽  
SHENGZHI MU ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Human Melanoma ◽  
Stat3 Signaling ◽  
Human Melanoma Cells

Abstract 2806: Oxidative stress limits metastasis of human melanoma cells

2016 ◽  
Author(s):  
Elena Piskounova ◽  
Michail Agathocleous ◽  
Malea Murphy ◽  
Zeping Hu ◽  
Ralph DeBerardinis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells

scholarly journals Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells

2015 ◽  
Vol 35 (2) ◽  
pp. 1065-1074 ◽  
Author(s):  
GUANGMING ZHAO ◽  
XIAODONG HAN ◽  
SIWEN ZHENG ◽  
ZHEN LI ◽  
YANG SHA ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Mtor Signaling ◽  
Mtor Signaling Pathway ◽  
Human Melanoma Cells ◽  
Proliferation And Invasion

Disulfiram facilitates intracellular copper transport that causes Oxidative stress and cell death in human Melanoma cells.

2004 ◽  
Vol 17 (4) ◽  
pp. 444-445 ◽  
Author(s):  
D. Cen ◽  
D Brayton ◽  
B Shahandeh ◽  
M Al-Ghazi ◽  
S McNulty ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Copper Transport ◽  
Human Melanoma Cells ◽  
Intracellular Copper

NCAM regulates the proliferation, apoptosis, autophagy, EMT, and migration of human melanoma cells via the Src/Akt/mTOR/cofilin signaling pathway

2019 ◽  
Vol 121 (2) ◽  
pp. 1192-1204 ◽  
Author(s):  
Jing Li ◽  
Rui Yang ◽  
Haijie Yang ◽  
Sujuan Chen ◽  
Lei Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
And Migration
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