Identification of human spermatogenesis-related proteins by comparative proteomic analysis: a preliminary study

2008 ◽  
Vol 90 (4) ◽  
pp. 1109-1118 ◽  
Author(s):  
Ran Huo ◽  
Ying He ◽  
Chun Zhao ◽  
Xue-jiang Guo ◽  
Min Lin ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Preliminary Study ◽  
Human Spermatogenesis ◽  
Related Proteins

scholarly journals Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

2018 ◽  
Vol 19 (12) ◽  
pp. 4020 ◽  
Author(s):  
Xinbo Wang ◽  
Yanhua Xu ◽  
Jingjing Li ◽  
Yongzhe Ren ◽  
Zhiqiang Wang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Gene Silencing ◽  
Proteomic Analysis ◽  
Crop Yields ◽  
Stress Conditions ◽  
Virus Induced Gene Silencing ◽  
Knock Down ◽  
Comparative Proteomic Analysis ◽  
Related Proteins

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

scholarly journals Silibinin Ameliorates O-GlcNAcylation and Inflammation in a Mouse Model of Nonalcoholic Steatohepatitis

2018 ◽  
Vol 19 (8) ◽  
pp. 2165 ◽  
Author(s):  
Su Lee ◽  
Min Nam ◽  
Da Lee ◽  
Jeen-Woo Park ◽  
Beom Kang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Protein Disulfide Isomerase ◽  
Therapeutic Agent ◽  
Hepatic Inflammation ◽  
Disulfide Isomerase ◽  
Nash Model ◽  
Comparative Proteomic Analysis ◽  
Related Proteins ◽  
Protein Disulfide ◽  
Promising Therapeutic Agent

The mechanisms underlying the progression to non-alcoholic steatohepatitis (NASH) remain to be elucidated. In the present study, we aimed to identify the proteins involved in the pathogenesis of liver tissue inflammation and to investigate the effects of silibinin, a natural polyphenolic flavonoid, on steatohepatitis. We performed comparative proteomic analysis using methionine and choline-deficient (MCD) diet-induced NASH model mice. Eighteen proteins were identified from the two-dimensional proteomic analysis, which are not only differentially expressed, but also significantly improved, by silibinin treatment. Interestingly, seven of these proteins, including keratin cytoskeletal 8 and 18, peroxiredoxin-4, and protein disulfide isomerase, are known to undergo GlcNAcylation modification, most of which are related to structural and stress-related proteins in NASH model animals. Thus, we primarily focused on how the GlcNAc modification of these proteins is involved in the progression to NASH. Remarkably, silibinin treatment alleviates the severity of hepatic inflammation along with O-GlcNAcylation in steatohepatitis. In particular, the reduction of inflammation by silibinin is due to the inhibition of the O-GlcNAcylation-dependent NF-κB-signaling pathway. Therefore, silibinin is a promising therapeutic agent for hyper-O-GlcNAcylation as well as NASH.

scholarly journals Comparative proteomic analysis reveals alterations in development and photosynthesis-related proteins in diploid and triploid rice

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Shuzhen Wang ◽  
Wenyue Chen ◽  
Changdeng Yang ◽  
Jian Yao ◽  
Wenfei Xiao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Related Proteins
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