Transcription factor klf12 negatively regulates estradiol synthesis in ovarian granulosa cells

2014 ◽  
Vol 102 (3) ◽  
pp. e272
Author(s):  
Y. Jiang ◽  
B. Wang ◽  
Y. Hu ◽  
H. Sun ◽  
G. Yan
Keyword(s):  
Transcription Factor ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Estradiol Synthesis

RAF1 as Downstream Molecule Mediates the FSH Signaling Pathway to Stimulate E2 Synthesis and Secretion in Mouse Ovarian Granulosa Cells

2020 ◽  
Author(s):  
Xuan Luo ◽  
Hui Liu ◽  
Hongzhou Guo ◽  
Longjie Sun ◽  
Kemian Gou ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Granulosa Cells ◽  
Regulatory Function ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells ◽  
Erk Phosphorylation ◽  
Extracellular Signal ◽  
Key Factor ◽  
Cytochrome P450 Family ◽  
Estradiol Synthesis

Abstract Background: V-raf-leukemia viral oncogene 1 (RAF1) kinase is the key factor in extracellular signal regulated pathway, which transmits signals to the downstream extracellular regulated protein kinases (ERK). Regulatory function of RAF1 has been proved to mediate steroid hormone synthesis, which played an essential physiological function in reproduction and development. Whether RAF1 takes part in the signaling events of gonadotropic hormones follicle-stimulating hormone (FSH) in ovarian is unknown.Results: We found that RAF1 as downstream molecule mediates the FSH signaling pathway to stimulate estradiol (E2) synthesis and secretion in mouse ovarian granulosa cells (GCs). The expression of RAF1 is induced by FSH and the production of E2 is increased in the serum and primary ovarian GCs supernatant, the process of which is blocked by treating with RAF1 inhibitor (N-(2-Methyl-5'-morpholino-6'-((tetrahydro-2H-pyran-4-yl)oxy)-[3,3'-bipyridin]-5-yl)-3(trifluoromethyl) benzamide, RAF709). Inhibition of RAF1 activity by RAF709 decreased ERK phosphorylation, and suppressed the expression of cytochrome P450 family 19 subfamily a member 1 (CYP19A1) which is a major rate-limiting enzyme to participate in the last step of E2 biosynthesis. Conclusion: Our results suggest that RAF1 play a pivotal mediating roles toward E2 production in FSH signaling pathway by inducing the phosphorylation of ERK and promoting the process of estradiol synthesis. RAF1 may be a potential and effective factor to regulate the function of the female mouse reproductive system.

Local effect of bisphenol A on the estradiol synthesis of ovarian granulosa cells from PCOS

2016 ◽  
Vol 33 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yuan Wang ◽  
Qinling Zhu ◽  
Xuan Dang ◽  
Yaqiong He ◽  
Xiaoxue Li ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Local Effect ◽  
Ovarian Granulosa Cells ◽  
Estradiol Synthesis

Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes

2018 ◽  
Author(s):  
Ravi, P.S.P. Gupta, S. Nandi, S. Mondal, Kumar Soni­ ◽  
P.S.P. Gupta ◽  
S. Nandi ◽  
S. Mondal, J.R. Ippala, Avantika Mor, A Mondal ◽  
J.R. Ippala ◽  
...  
Keyword(s):  
Cell Survival ◽  
Mrna Expression ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Culture Medium ◽  
Estrus Synchronization ◽  
Ovarian Granulosa Cells ◽  
Effect Of Copper ◽  
Estradiol Synthesis

The study was conducted by supplementing cupric chloride dihydrate to modulate the estradiol synthesis in granulosa cells with a hypothesis of possible use of copper to potentiate or partially replace the hormones for estrus induction / estrus synchronization in future studies. In present study copper at three doses (0.1, 0.5 and 1 mM level in culture medium) were tested to deserve see their effects on in vitro granulosa cell survival, estradiol synthesis and their associated genes of ovarian granulosa cells of goat.There was no effect of copper on the ovarian granulosa cell survival rate. There was a considerable increase in the estradiol level per ml culture medium basis by 6th day of in vitro culture with the second dose of copper i.e. 0.5 mM, but the increase was non-significant (P greator than 0.05). There was no significant effect of copper on estradiol synthesis when expressed on per 30000 cell basis. Effect of copper (0.1 mM and 0.5 mM) on the mRNA expression of genes of aromatase (CYP19A1) and cyclin D2 (CCND2) was estimated. Copper had significantly (P less than 0.05) increased the mRNA expression of CCND2 and CYP19A1in ovarian granulosa cells with only one of the two doses tested i.e. 0.5 mM. Hence, copper can be considered as a potential mineral to supplement along with hormones in estrus induction or estrus synchronization protocols to minimize the use of hormones.

scholarly journals Author response: The transcription factor FOXL2 mobilizes estrogen signaling to maintain the identity of ovarian granulosa cells

2014 ◽  
Author(s):  
Adrien Georges ◽  
David L'Hôte ◽  
Anne Laure Todeschini ◽  
Aurélie Auguste ◽  
Bérangère Legois ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Granulosa Cells ◽  
Author Response ◽  
Estrogen Signaling ◽  
Ovarian Granulosa Cells

scholarly journals TRIM28-dependent SUMOylation protects the adult ovary from the male pathway

2021 ◽  
Author(s):  
Moïra Rossitto ◽  
Stephanie Dejardin ◽  
Chris M Rands ◽  
Stephanie Legras ◽  
Roberta Migale ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Fate ◽  
Granulosa Cells ◽  
Sertoli Cells ◽  
Ovary Cell ◽  
Protective Mechanism ◽  
Intermediate Cell ◽  
Post Translational Modification ◽  
Ovarian Granulosa Cells ◽  
Adult Ovary

Gonadal sexual fate in mammals is determined during embryonic development and must be actively maintained in adulthood. Therefore, gonadal sex-specific transcription factors are required to prevent transdifferentiation of gonadal somatic cells to the other sexual fate. Mouse genetic experiments have shown that oestrogen receptor signalling and the transcription factor FOXL2 protect ovarian granulosa cells from transdifferentiation into Sertoli cells, their testicular counterpart. However, the mechanism underlying this protective mechanism is unknown. Here, we show that one post-translational modification (i.e. SUMOylation catalysed by TRIM28) is sufficient to prevent female-to-male sex reversal of the mouse ovary after birth. We found that upon loss of TRIM28 SUMO-E3 ligase activity, ovarian granulosa cells transdifferentiate to Sertoli cells through an intermediate cell type different from gonadal embryonic progenitors. TRIM28 binds to chromatin close to the critical transcription factor FOXL2 to maintain the female pathway through SUMOylation of specific chromatin regions. Therefore, FOXL2 signalling might maintain the adult ovary cell fate via TRIM28-dependent SUMOylation. Improper SUMOylation of chromatin regions in granulosa cells might lead to female reproductive disorders and infertility, the incidence of which is currently increasing.

MiR-126* is a novel functional target of transcription factor SMAD4 in ovarian granulosa cells

Gene ◽  
2019 ◽  
Vol 711 ◽  
pp. 143953 ◽  
Author(s):  
Qiqi Li ◽  
Xing Du ◽  
Lu Liu ◽  
Zengxiang Pan ◽  
Shaoxian Cao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells
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