Altered mouse oocyte DNA methyltransferases (Dnmts) after vitrification with dimethyl sulfoxide (DMSO)

DNA from mammalian cells has been shown to contain significant amounts of 5-methyl cytosine resulting from enzymatic transfer of methyl groups from s-adenosylmethionine to cytosine residues in the DNA polymer. The function of this modification is not known. We have found that DNA synthesized during chemically induced differentiation of friend erythroleukemia cells is hypomethylated, as measured by its ability to accept methyl groups transferred by homologous DNA methyltransferases in vitro. The extent of hypomethylation detected by this sensitive method is small, a decrease of less than 1.6 percent in 5-methylcytosine content. Hypomethylated DNA can be isolated from friend erythroleukemia cells grown in the presence of dimethyl sulfoxide, butyrate, hexamethylene-bis- acetamide, pentamethylene-bis acetamide, and ethionine. However, hypomethylated DNA is found only under conditions where differentiation is actually induced. DNA isolated from cells of a dimethyl sulfoxide- resistant subclone grown in the presence of that agent is not hypomethylated, although DNA of these cells becomes hypomethylated after growth in the presence of inducers that can trigger their differentiation. We also find that the DNA of friend erythroleukemia cells does not become hypomethylated when the cells are exposed to inducing agents in the presence of substances that inhibit differentiation. These results suggest a close link between genome modification by methylation and differentiation of friend erythroleukemia cells.

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Mouse Oocyte Methylomes at Base Resolution Reveal Genome-Wide Accumulation of Non-CpG Methylation and Role of DNA Methyltransferases

PLoS Genetics ◽

10.1371/journal.pgen.1003439 ◽

2013 ◽

Vol 9 (4) ◽

pp. e1003439 ◽

Cited By ~ 180

Author(s):

Kenjiro Shirane ◽

Hidehiro Toh ◽

Hisato Kobayashi ◽

Fumihito Miura ◽

Hatsune Chiba ◽

...

Keyword(s):

Dna Methyltransferases ◽

Cpg Methylation ◽

Mouse Oocyte ◽

Genome Wide

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Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations

Canadian Journal of Animal Science ◽

10.4141/cjas10042 ◽

2011 ◽

Vol 91 (3) ◽

pp. 385-388

Author(s):

Afrooz Habibi ◽

Ahmad Hosseini ◽

Naser Farrokhi ◽

Fardin Amidi ◽

Isabel Carvalhais ◽

...

Keyword(s):

Ethylene Glycol ◽

Dimethyl Sulfoxide ◽

Short Communication ◽

Mouse Oocyte ◽

Toxic Effects ◽

Mouse Oocytes ◽

Vitrification Solution

Habibi, A., Hosseini, A., Farrokhi, N., Amidi, F., Carvalhais, I., Chaveiro, A. and Moreira da Silva, F. 2011. Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations. Can. J. Anim. Sci. 91: 385–388. The response of vitrified mouse MII oocytes in the presence of two concentrations of cryoprotectants [vit1 (15%: 7.5% dimethyl sulfoxide (DMSO)+7.5% ethylene glycol (EG) and vit2 (30%: 15% DMSO+15% EG)] was analyzed to investigate whether reducing cryoprotectant concentrations can affect oocyte survival after cryopreservation by the cryotop method. After thawing the survival, fertilization, cleavage and blastocyst rates were compared with unfrozen oocytes. It can be concluded that 15% cryoprotectant (7.5% DMSO+7.5% EG), instead of the commonly used 30% (15% DMSO+15% EG), could be helpful by moderating the probable toxic effects of vitrification solution in mouse oocyte during vitrification by cryotop.

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Mouse oocyte vitrification with and without dimethyl sulfoxide: influence on cryo-survival, development, and maternal imprinted gene expression

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-021-02221-1 ◽

2021 ◽

Author(s):

Clementina Cantatore ◽

Jenny S. George ◽

Raffaella Depalo ◽

Giuseppe D’Amato ◽

Molly Moravek ◽

...

Keyword(s):

Gene Expression ◽

Dimethyl Sulfoxide ◽

Mouse Oocyte ◽

Oocyte Vitrification ◽

Imprinted Gene

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Dimethyl sulfoxide as a vehicle for corticosteroids. A comparison with the occlusive dressing technique

Archives of Dermatology ◽

10.1001/archderm.97.2.110 ◽

1968 ◽

Vol 97 (2) ◽

pp. 110-114 ◽

Cited By ~ 2

Author(s):

J. T. Groel

Keyword(s):

Dimethyl Sulfoxide ◽

Occlusive Dressing

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The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648027 ◽

1976 ◽

Vol 36 (01) ◽

pp. 221-229 ◽

Cited By ~ 16

Author(s):

Charles A. Schiffer ◽

Caroline L. Whitaker ◽

Morton Schmukler ◽

Joseph Aisner ◽

Steven L. Hilbert

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Dimethyl Sulfoxide ◽

Platelet Function ◽

Platelet Volume ◽

Short Term ◽

Platelet Factor ◽

Short Term Exposure ◽

Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

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Preservation of Morphological Integrity and Clot Retraction Activity of Human Platelets After Freezing

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654822 ◽

1964 ◽

Vol 11 (01) ◽

pp. 222-229 ◽

Cited By ~ 8

Author(s):

Isaac Djerassi ◽

Albert Roy ◽

Jorge Alvarado ◽

Keyword(s):

Dimethyl Sulfoxide ◽

Liquid Nitrogen ◽

Human Platelets ◽

Slow Freezing ◽

Critical Conditions ◽

Plasma Medium ◽

Clot Retraction ◽

Controlled Freezing ◽

Direct Immersion

SummaryHuman platelets frozen at −195° C (liquid nitrogen) retain their morphological integrity and ability to promote clot retraction when 5% dimethyl-sulfoxide and 5% dextrose are added to the suspending plasma medium. Slow freezing was more effective than direct immersion in the liquid nitrogen. Although similar results may be achieved with dimethylsulfoxide alone with rigidly controlled freezing rates, the addition of sugars may permit freezing under less critical conditions.Dimethylsulfoxyd und 5% Dextrose dem Plasmamilieu hinzugefügt werden. Das langsame Einfrieren ist effektiver als das direkte Eintauchen in flüssigen Stickstoff. Obschon ähnliche Resultate mit Dimethylsulfoxyd allein unter exakter Kontrolle der Einfrierungsgeschwindig-keit erreicht werden können, erlaubt die Zugabe von Dextrose ein Einfrieren unter weniger kritischen Bedingungen.

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