scholarly journals Can timing of cell exclusion identified in a time-lapse culture system be a predictor for embryo ploidy?

2018 ◽  
Vol 110 (4) ◽  
pp. e359
Author(s):  
D.L. Walker ◽  
K.M. Barud ◽  
R. Reiter ◽  
J. Fredrickson ◽  
C.C. Shenoy ◽  
...  
Keyword(s):  
Culture System ◽  
Time Lapse

Growth of the Inner Ear in Organ Culture

1974 ◽  
Vol 83 (14_suppl) ◽  
pp. 1-16 ◽  
Author(s):  
Thomas Van De Water ◽  
R. J. Ruben
Keyword(s):  
Organ Culture ◽  
Inner Ear ◽  
Culture System ◽  
Time Lapse ◽  
Research Tool ◽  
Abnormal Development ◽  
Morphological Development ◽  
Histological Techniques ◽  
Organ Culture System

An organ culture system is presented for the mammalian inner ear. The development of the inner ear is recorded with time lapse cinematography and conventional histological techniques. The system allows for morphological development of differentiation of the neurosensory structures of the inner ear. The use of the organ culture system as a research tool for the understanding of normal and abnormal development of the inner ear is discussed.

Growth of the Inner Ear in Organ Culture

1974 ◽  
Vol 83 (5_suppl) ◽  
pp. 1-16 ◽  
Author(s):  
Thomas Van De Water ◽  
R. J. Ruben
Keyword(s):  
Organ Culture ◽  
Inner Ear ◽  
Culture System ◽  
Time Lapse ◽  
Research Tool ◽  
Abnormal Development ◽  
Morphological Development ◽  
Histological Techniques ◽  
Organ Culture System

An organ culture system is presented for the mammalian inner ear. The development of the inner ear is recorded with time lapse cinematography and conventional histological techniques. The system allows for morphological development of differentiation of the neurosensory structures of the inner ear. The use of the organ culture system as a research tool for the understanding of normal and abnormal development of the inner ear is discussed.

A comparison of embryo development and clinical outcome using autologous endomentrial coculture and time-lapse culture system

2014 ◽  
Vol 102 (3) ◽  
pp. e213
Author(s):  
Q. Zhan ◽  
N. Zaninovic ◽  
C.M. Norberg ◽  
J. Hao ◽  
Z. Ye ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Embryo Development ◽  
Culture System ◽  
Time Lapse

scholarly journals Time-Lapse Cinematography-Compatible Polystyrene-Based Microwell Culture System: A Novel Tool for Tracking the Development of Individual Bovine Embryos1

2010 ◽  
Vol 83 (6) ◽  
pp. 970-978 ◽  
Author(s):  
Satoshi Sugimura ◽  
Tomonori Akai ◽  
Tamás Somfai ◽  
Muneyuki Hirayama ◽  
Yoshio Aikawa ◽  
...  
Keyword(s):  
Culture System ◽  
Time Lapse ◽  
System A

scholarly journals The Inclusion Principles of Human Embryos in the WOW-Based Time-Lapse System: A Retrospective Cohort Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuqiong Wang ◽  
Sheng Wang ◽  
Xilin Qian ◽  
Yanrong Kuai ◽  
Yang Xu
Keyword(s):  
Culture System ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Individual Identification ◽  
Human Embryos ◽  
Paracrine Signaling ◽  
Negative Effects ◽  
Group Culture ◽  
System A ◽  
Negative Effect

A time-lapse system (TLS) with a well-of-the-well (WOW) dish, which allows individual identification and the possibility of autocrine and paracrine signaling between group-cultured embryos, has been widely used in clinic. However, there is a need to re-think the inclusion principles of human embryos in WOW-based TLS, especially for grade IV (G4) embryos, which are considered to potentially have detrimental effects on surrounding embryos. Here, we carried out a single-center, large-cohort, retrospective study, comprising 303 patients undergoing IVF (148 cases) and ICSI (155 cases), with a total of 3282 embryos, to compare embryonic development until the blastocyst stage in the group culture system with or without G4 embryos. Further, LC-MS/MS was used to analyze the G1-G4 embryo secretome to understand the influence of G4 embryos on the group culture microenvironment. We proved that polypronuclear (PPN) embryos positively contribute to the development of the neighboring embryos through secretion of ILIAP, ITI-H4, and keratin. Existence of more than one G4 embryo had a negative effect on the other embryos (p < 0.05). Moreover, G4 embryos were found to secrete KLKB1 and VTDB, which might harm the neighboring embryos. Thus, our study clarified that when embryos are subjected to group culture in WOW-based TLS, the PPN-derived embryos need not be removed, and it is important to ensure that no more than one G4 embryo is present to avoid negative effects on the neighboring embryos.

scholarly journals A novel zona-free culture system for severe cytoplasmic fragmentation cases: a pilot study using 3PN embryos and time-lapse cinematography

2019 ◽  
Vol 112 (3) ◽  
pp. e124-e125
Author(s):  
Keitaro Yumoto ◽  
Toko Shimura ◽  
Minako Sugishima ◽  
Yasuyuki Mio
Keyword(s):  
Pilot Study ◽  
Culture System ◽  
Time Lapse

138 TIME-LAPSE CINEMATOGRAPHY-COMPATIBLE INJECTION-MOLDED MICROWELL CULTURE SYSTEM FOR TRACKING THE DEVELOPMENT OF INDIVIDUAL BOVINE EMBRYOS

2011 ◽  
Vol 23 (1) ◽  
pp. 173
Author(s):  
S. Sugimura ◽  
T. Akai ◽  
T. Somfai ◽  
M. Hirayama ◽  
Y. Aikawa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Culture System ◽  
Cell Mass ◽  
Calf Serum ◽  
Time Lapse ◽  
Pregnancy Rates ◽  
Cleavage Pattern ◽  
Bovine Embryos ◽  
Physiological Level

We have developed a polystyrene-based well of-the-well system (WOW) using injection moulding to track individual embryos throughout culture using time-lapse cinematography (TLC). The WOW-cultured bovine embryos following in vitro fertilization (IVF) were compared with conventional droplet (control)-cultured embryos on in vitro and in vivo development. Twenty-five of zygotes were cultured in each culture system containing 125 μL of CR1aa medium supplemented with 5% calf serum for 168 h after IVF. No differences between control- and WOW-cultured embryos were observed during development to the blastocyst stage. Morphological quality, inner cell mass (ICM), and trophectoderm (TE) cell numbers and post-vitrification survival rates were not different between control- and WOW-derived blastocysts; however, incidence of apoptosis in the ICM and TE cells was reduced in WOW culture (P < 0.01). Oxygen consumption in WOW-derived blastocysts was closer to physiological level than that of control-derived blastocysts. Moreover, WOW culture improved embryo viability, as indicated by increased pregnancy rates at days 30 and 60 after embryo transfer (P < 0.05). The TLC monitoring was performed to evaluate the cleavage pattern and the duration of the first cell cycle of embryos from oocytes collected by ovum pick-up; correlations with success of pregnancy were determined. Logistic regression analysis indicated that the cleavage pattern correlated with success of pregnancy (P < 0.05), but cell cycle length did not. Higher pregnancy rates (66.7%) were observed for animals in which transferred blastocysts had undergone normal cleavage, identified by the presence of 2 blastomeres of the same size without fragmentation, than among those with abnormal cleavage (33.3%). These results suggest that our microwell culture system is a powerful tool for producing and selecting healthy embryos and for identifying viability biomarkers. This work was supported by the Research and Developmental Program for New Bio-Industry Initiatives.

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