Protective effect of Codium fragile against UVB-induced pro-inflammatory and oxidative damages in HaCaT cells and BALB/c mice

Pomegranate extract (PG-E) has been reported to exert a protective effect on the skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions, and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in different biotechnological applications. Thus, we investigated the capacity of Brassica oleracea L. (cauliflower) inflorescence vesicles (CI-vesicles) to encapsulate PG-E and determined the stability and the antioxidant capacity of the system over time. In addition, the protective effect against UV radiation and heavy metals in HaCaT cells was also tested. The CI-vesicles had an entrapment efficiency of around 50%, and accelerated stability tests did not show significant changes in the parameters tested. The results for the HaCaT cells showed the non-cytotoxicity of the CI-vesicles containing PG-E and their protection against heavy metals (lead acetate and mercuric chloride) and UV-B radiation through a reduction of oxidative stress. The reduction of the percentage of deleted mtDNA (mtDNA4977, “common deletion”) in UV-treated HaCaT cells due to the presence of CI-vesicles containing PG-E indicated the mechanism of protection. Therefore, the effects of CI-vesicles loaded with PG-E against oxidative stress support their utilization as natural cosmeceuticals to protect skin health against external damage from environmental pollution and UV radiation.

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Protective effect of astragaloside IV on oxidative damages of Chang Liver cell induced by ethanol and H2O2

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20142229 ◽

2014 ◽

Keyword(s):

Protective Effect ◽

Liver Cell ◽

Astragaloside Iv ◽

Chang Liver Cell ◽

Oxidative Damages ◽

Chang Liver

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Nanoencapsulation of Pomegranate Extract to Increase Stability and Potential Dermatological Protection

10.20944/preprints202012.0432.v1 ◽

2020 ◽

Author(s):

Lucia Yepes-Molina ◽

Jose Antonio Hernandez ◽

Micaela Carvajal

Keyword(s):

Oxidative Stress ◽

Heavy Metals ◽

Protective Effect ◽

Uv Radiation ◽

Entrapment Efficiency ◽

Hacat Cells ◽

Biotechnological Applications ◽

Accelerated Stability ◽

The Stability ◽

Brassica Oleracea L

Pomegranate extract (PG-E) has been reported to exert a protective effect in skin due to its antioxidant activity. Ingredients rich in phenolic compounds are unstable in extract solutions and, therefore, the use of a suitable nanosystem to encapsulate this type of extract could be necessary in different biotechnological applications. Thus, we investigated the capacity of Brassica oleracea L. (cauliflower) inflorescence vesicles (CI-vesicles) to encapsulate PG-E and determined the stability and the antioxidant capacity of the system over time. In addition, the protective effect against UV radiation and heavy metals in HaCaT cells was also tested. The CI-vesicles had an entrapment efficiency around 50% and accelerated stability tests did not show significant changes in the parameters tested. The results for the HaCaT cells show the non-cytotoxicity of the CI-vesicles containing PG-E and their protection against heavy metals (lead acetate and mercuric chloride) and UV-B radiation through a reduction of oxidative stress. The reduction of the percentage of deleted mtDNA (mtDNA4977, “common deletion”) in UV-treated HaCaT cells due to the presence of CI-vesicles containing PG-E indicates the mechanism of protection. Therefore, the effects of CI-vesicles loaded with PG-E against oxidative stress support their utilization as natural cosmeceuticals to protect skin health against external damage from environmental pollution and UV radiation.

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Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells)

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2014.01.013 ◽

2014 ◽

Vol 132 ◽

pp. 27-35 ◽

Cited By ~ 35

Author(s):

Rossella Calò ◽

Laura Marabini

Keyword(s):

Protective Effect ◽

Cell Line ◽

Vaccinium Myrtillus ◽

Hacat Cells ◽

Human Keratinocyte ◽

Human Keratinocyte Cell Line ◽

Keratinocyte Cell Line Hacat ◽

Keratinocyte Cell

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Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish

Marine Drugs ◽

10.3390/md18060316 ◽

2020 ◽

Vol 18 (6) ◽

pp. 316 ◽

Cited By ~ 2

Author(s):

Wanchun Su ◽

Lei Wang ◽

Xiaoting Fu ◽

Liying Ni ◽

Delin Duan ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Protective Effect ◽

Reactive Oxygen ◽

Saccharina Japonica ◽

Intracellular Reactive Oxygen Species ◽

Dependent Manner ◽

Hacat Cells ◽

Oxygen Species

A fucose-rich fucoidan was purified from brown seaweed Saccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.

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