scholarly journals AI-aided on-chip Nucleic Acid Assay for Smart Diagnosis of Infectious Disease

2021 ◽  
Author(s):  
Hao Sun ◽  
Linghu Xiong ◽  
Yi Huang ◽  
Xinkai Chen ◽  
Yongjian Yu ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Nucleic Acid ◽  
Nucleic Acid Assay ◽  
On Chip

scholarly journals Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kanako Saito ◽  
Yuri Ota ◽  
Dieter M. Tourlousse ◽  
Satoko Matsukura ◽  
Hirotsugu Fujitani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Purity ◽  
Droplet Microfluidics ◽  
Individual Growth ◽  
Proof Of Concept ◽  
Widespread Adoption ◽  
Technical Challenges ◽  
On Chip ◽  
Uncultured Microorganisms ◽  
Environmental Microbes

AbstractDroplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

Multifunctional Printable Micropattern Array for Digital Nucleic Acid Assay for Microbial Pathogen Detection

2021 ◽  
Vol 13 (2) ◽  
pp. 3098-3108
Author(s):  
Yunho Choi ◽  
Younseong Song ◽  
Yong Tae Kim ◽  
Seok Jae Lee ◽  
Kyoung G. Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Microbial Pathogen ◽  
Nucleic Acid Assay

Ultrasensitive Nucleic Acid Assay Based on Cyclometalated Iridium(III) Complex with High Electrochemiluminescence Efficiency

2020 ◽  
Author(s):  
Zhi-Hong Xu ◽  
Hang Gao ◽  
Nan Zhang ◽  
Wei Zhao ◽  
Yi-Xiang Cheng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Assay

scholarly journals Screening Metagenomic Data for Viruses Using the E-Probe Diagnostic Nucleic Acid Assay

2014 ◽  
Vol 104 (10) ◽  
pp. 1125-1129 ◽  
Author(s):  
A. H. Stobbe ◽  
W. L. Schneider ◽  
P. R. Hoyt ◽  
U. Melcher
Keyword(s):  
Nucleic Acid ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Metagenomic Data ◽  
Data Sets ◽  
Virus Species ◽  
Data Set ◽  
Golden Yellow ◽  
Nucleic Acid Assay ◽  
Ngs Data

Next generation sequencing (NGS) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a NGS data set. By using the unassembled NGS data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. This method, designated e-probe diagnostic nucleic acid assay, first tested with mock sequence databases, was tested with NGS data sets generated from plants infected with a DNA (Bean golden yellow mosaic virus, BGYMV) or an RNA (Plum pox virus, PPV) virus. In addition, the ability to detect and differentiate among strains of a single virus species, PPV, was examined by using probe sets that were specific to strains. The use of probe sets for multiple viruses determined that one sample was dually infected with BGYMV and Bean golden mosaic virus.

scholarly journals Plasmon-enhanced fluorescence imaging with silicon-based silver chips for protein and nucleic acid assay

2017 ◽  
Vol 955 ◽  
pp. 98-107 ◽  
Author(s):  
Bing Yuan ◽  
Xiangxu Jiang ◽  
Chu Yao ◽  
Meimei Bao ◽  
Jiaojiao Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Enhanced Fluorescence ◽  
Plasmon Enhanced Fluorescence ◽  
Nucleic Acid Assay ◽  
Silicon Based

scholarly journals Magnetic Particles for Advanced Molecular Diagnosis

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2158 ◽  
Author(s):  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu ◽  
Alina Maria Holban
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles ◽  
Early Stage ◽  
Disease Diagnosis ◽  
Laboratory Medicine ◽  
Clinical Reality

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

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