Nucleic acid amplification and infectious disease

1994 ◽  
Vol 25 (6) ◽  
pp. 591-593 ◽  
Author(s):  
Beverly Barton Rogers
Author(s):  
Caitlin M. Dugdale ◽  
Sarah E. Turbett ◽  
Suzanne M. McCluskey ◽  
Kimon C. Zachary ◽  
Erica S. Shenoy ◽  
...  

Abstract We describe an approach to the evaluation and isolation of hospitalized persons under investigation (PUIs) for coronavirus disease 2019 (COVID-19) at a large US academic medical center. Only a small proportion (2.9%) of PUIs with 1 or more repeated severe acute respiratory coronavirus virus 2 (SARS-CoV-2) nucleic acid amplification tests (NAATs) after a negative NAAT were diagnosed with COVID-19.


Lab on a Chip ◽  
2017 ◽  
Vol 17 (14) ◽  
pp. 2347-2371 ◽  
Author(s):  
Laura Magro ◽  
Camille Escadafal ◽  
Pierre Garneret ◽  
Béatrice Jacquelin ◽  
Aurélia Kwasiborski ◽  
...  

On-field infectious disease diagnostics can be performed with paper microfluidics through sample preparation and nucleic acid amplification.


2016 ◽  
Vol 3 (2) ◽  
pp. 145
Author(s):  
Anggun Feranisa

Background: Molecular diagnostic is an emerging diagnostic method inpersonalized medicine/dentistry era. Usually, it uses nucleic acid amplificationmethod to detect various diseases. PCR is conventional nucleic acid amplification method. However, due to an urgency in infectious diseases’ diagnotic method, scientists developed LAMP as new nucleic acid amplification method.Discussion: There are various experiments used to develop LAMP as infectious diseases diagnostic method compared to PCR. The results are LAMP more sensitive, specific, rapid, and inexpensive than PCR.Conclusion: Both PCR and LAMP can be used as molecular diagnostic tools.LAMP prefer to used as infectious disease diagnostic method in poor anddeveloping countries.


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