Development of a lateral flow colloidal gold immunoassay strip for the simultaneous detection of Shigella boydii and Escherichia coli O157:H7 in bread, milk and jelly samples

Food Control ◽  
2016 ◽  
Vol 59 ◽  
pp. 345-351 ◽  
Author(s):  
Chunmei Song ◽  
Cheng Liu ◽  
Shuyan Wu ◽  
Haolin Li ◽  
Huiqin Guo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Escherichia Coli O157 ◽  
Shigella Boydii

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62300-62305 ◽  
Author(s):  
Jing-Yun Wang ◽  
Ming-Hui Chen ◽  
Zhi-Chao Sheng ◽  
Dao-Feng Liu ◽  
Song-Song Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Test Strip ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7.

A gold nanoparticles-assisted multiplex PCR assay for simultaneous detection of Salmonella typhimurium, Listeria monocytogenes and Escherichia coli O157:H7

2020 ◽  
Vol 12 (2) ◽  
pp. 212-217 ◽  
Author(s):  
Juan Du ◽  
Shujing Wu ◽  
Liyuan Niu ◽  
Junguang Li ◽  
Dianbo Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Escherichia Coli O157 ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pcr Product

Unfunctionalized flower-shaped AuNPs is used as colorimetric sensor for PCR product detection by naked eyes.

scholarly journals Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays

Toxins ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 92 ◽  
Author(s):  
Jinliang Wang ◽  
Robab Katani ◽  
Lingling Li ◽  
Narasimha Hegde ◽  
Elisabeth Roberts ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Escherichia Coli O157 ◽  
Shiga Toxins

A remarkable sensitivity enhancement in a gold nanoparticle-based lateral flow immunoassay for the detection of Escherichia coli O157:H7

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45092-45097 ◽  
Author(s):  
Xi Cui ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Lei Zhang ◽  
Yun Rong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticle ◽  
Quantitative Data ◽  
Visual Inspection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

The size and uniformity of AuNPs were optimized to maximally amplify both visual inspection signals and quantitative data of LFA.

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