Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 62300-62305 ◽  
Author(s):  
Jing-Yun Wang ◽  
Ming-Hui Chen ◽  
Zhi-Chao Sheng ◽  
Dao-Feng Liu ◽  
Song-Song Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Test Strip ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Poly-L-lysine brushes on magnetic nanoparticles for ultrasensitive detection of Escherichia coli O157: H7

Talanta ◽  
2017 ◽  
Vol 172 ◽  
pp. 53-60 ◽  
Author(s):  
Jing Wu ◽  
Yan Chen ◽  
Yu Wang ◽  
Hong Yin ◽  
Zhitao Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Magnetic Nanoparticles ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157

scholarly journals Immunomagnetic separation and size-based detection of Escherichia coli O157 at the meniscus of a membrane strip

RSC Advances ◽  
2018 ◽  
Vol 8 (46) ◽  
pp. 26266-26270 ◽  
Author(s):  
Hyeonjeong Lee ◽  
Jeongin Hwang ◽  
Yunsung Park ◽  
Donghoon Kwon ◽  
Sanghee Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Velocity ◽  
Test Strip ◽  
Immunomagnetic Separation ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Membrane Strip

E. coli–Au/MNC complexes accumulate at the meniscus of the test strip where the flow velocity reaches a maximum.

QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold

2011 ◽  
Vol 26 (7) ◽  
pp. 3376-3381 ◽  
Author(s):  
Zhi-Qiang Shen ◽  
Jing-Feng Wang ◽  
Zhi-Gang Qiu ◽  
Min Jin ◽  
Xin-Wei Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colloidal Gold ◽  
Escherichia Coli O157 ◽  
Catalytic Growth
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