Detection of stx1 and stx2 and subtyping of Shiga toxin-producing Escherichia coli using asymmetric PCR combined with lateral flow immunoassay

Food Control ◽  
2021 ◽  
pp. 108051
Author(s):  
Shan Shan ◽  
Yanmei Huang ◽  
Zhaohong Huang ◽  
Zhonger Long ◽  
Chengwei Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Asymmetric Pcr

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

A remarkable sensitivity enhancement in a gold nanoparticle-based lateral flow immunoassay for the detection of Escherichia coli O157:H7

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45092-45097 ◽  
Author(s):  
Xi Cui ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Lei Zhang ◽  
Yun Rong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticle ◽  
Quantitative Data ◽  
Visual Inspection ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

The size and uniformity of AuNPs were optimized to maximally amplify both visual inspection signals and quantitative data of LFA.

scholarly journals Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

2010 ◽  
Vol 399 (2) ◽  
pp. 831-838 ◽  
Author(s):  
P. Noguera ◽  
G. A. Posthuma-Trumpie ◽  
M. van Tuil ◽  
F. J. van der Wal ◽  
A. de Boer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Shiga Toxin ◽  
Carbon Nanoparticles ◽  
Lateral Flow ◽  
Genes Encoding

scholarly journals Lateral flow immunoassay integrated with competitive and sandwich models for the detection of aflatoxin M1 and Escherichia coli O157:H7 in milk

2018 ◽  
Vol 101 (10) ◽  
pp. 8767-8777 ◽  
Author(s):  
Chun Wang ◽  
Juan Peng ◽  
Dao-Feng Liu ◽  
Ke-Yu Xing ◽  
Gang-Gang Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lateral Flow ◽  
Aflatoxin M1 ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157

Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli

2013 ◽  
Vol 93 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Taro Yonekita ◽  
Ryuji Ohtsuki ◽  
Eri Hojo ◽  
Naoki Morishita ◽  
Takashi Matsumoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Peptide ◽  
Shiga Toxin ◽  
Lateral Flow ◽  
Lateral Flow Assay
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