Detection of GM soybean in food products by simultaneous employment of three pairs of PCR primers

2009 ◽  
Vol 42 (3) ◽  
pp. 349-352 ◽  
Author(s):  
Zorica Nikolić ◽  
Ksenija Taški-Ajduković ◽  
Aranka Jevtić ◽  
Dragana Marinković
Keyword(s):  
Food Products ◽  
Pcr Primers ◽  
Gm Soybean

scholarly journals Detection of Unlabeled Genetically Modified Soybean in the Omani Market

2016 ◽  
Vol 21 (1) ◽  
pp. 1
Author(s):  
Nabila Al-Sadqi ◽  
Aliya S. Alansari
Keyword(s):  
Real Time ◽  
Quantitative Methods ◽  
Genetically Modified ◽  
Food Products ◽  
Detection Methods ◽  
Soybean Plant ◽  
Roundup Ready ◽  
Specific Pcr ◽  
Roundup Ready Soybean ◽  
Gm Soybean

This study aimed to screen for products containing Genetically Modified (GM) food in the Omani market using detection methods for the presence of Roundup Ready Soybean, Bt176 and MON810 maize in food products and to quantify it in positive samples using real time Polymerase Chain Reaction (PCR). A total of 100 food samples were collected randomly from markets in Oman. Out of 59 samples, 8  (13.5%) were successfully amplified with the maize plant specific PCR. GM screening showed negative for all samples, which indicated low or no GM maize in the samples tested. Out of 57 soy containing samples, 40 (70%) were successfully amplified by the soybean plant specific PCR. Six samples out of the 40 (15%) were found positive for GM using P35S-cf3/P35S-cr4 and HA-nos118-f/HA-nos118-r, primer pairs and using GMO5/GMO9 and GMO7/GMO8 primer pairs for specific detection of Roundup Ready Soybean. Real time PCR (TaqMan™ system) was carried out for the positive Roundup Ready Soybean samples and results showed that 2 out of the positive GM soy samples contained more than 5%; a Soy Formula for Infants (imported) sample contained 21% GM soybean and raw soybean seeds (imported in bulk amounts and packed in Oman)  contained 88% GM soybean. The results demonstrate for the first time the presence of GM-soy in food products in the Omani market, reinforcing the need for the use of qualitative and quantitative methods for GM detection in food products.  

Problems of ensuring metrological traceability of measurement results of indicators of quality for food products and food raw materials

2020 ◽  
pp. 64-70
Author(s):  
Mariya Y. Medvedevskikh ◽  
Anna S. Sergeeva
Keyword(s):  
Reference Materials ◽  
Mass Fraction ◽  
Nutritional Value ◽  
Animal Feed ◽  
Raw Materials ◽  
Meat Products ◽  
Metrological Traceability ◽  
Food Products ◽  
Freeze Dried ◽  
Measurement Results

The article raises the problem of ensuring metrological traceability of the measurement results of indicators of quality and nutritional value for food products and food raw materials: water (moisture), nitrogen (protein, crude protein), fat, ash and carbohydrates. The problem under consideration can be solved by applying reference materials of food composition, traceable to state primary measurement standards GET 173-2017 and GET 176-2019 and primary reference measurement procedures (PRMP), for attestation of measurement procedures and accuracy checking of measurement results. The article discusses the results of the PRMP development of mass fraction of fat, ash and carbohydrates in food products and food raw materials, as well as mass fraction of crude fat (oil content) in oil crops seeds and products based on them. The paper also presents metrological characteristics of reference materials of composition of dry dairy products, grain-milk dry porridges for nutrition of babies, grain dry porridges for nutrition of babies, egg powder, freeze-dried meat products, animal feed. The results of the work allow for building a chain of metrological traceability from GET 173-2017, GET 176-2019 and PRMP to routine measurement procedures, thereby ensuring the uniformity of measurements of nutritional value of food products.

Use of seeds of seed hemp in specialized food Products

2020 ◽  
Vol 29 (7) ◽  
pp. 48-50
Author(s):  
A.A. Bubnova ◽  
Keyword(s):  
Food Products

Enhancement of food production quality: the truffle case

2020 ◽  
pp. 587-611 ◽  
Author(s):  
Elena Viganò ◽  
Federico Gori ◽  
Antonella Amicucci
Keyword(s):  
Food Production ◽  
Food Products ◽  
Production Quality ◽  
Regulatory Framework ◽  
Marketing Literature

The central role of quality agri-food production in the promotion of a given territory is actually widely recognized by both the economic and marketing literature and the stakeholders involved in the enhancement process of rural systems. On this basis, this work analyzes one of the finest Italian agri-food products: the truffle. This work tries to point out the main problems characterizing the current regulatory framework, the trade and the production of the Italian truffle sector, emphasizing their causes, consequences and possible solutions.

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