Effect of thermal processing on the performance of the novel single-tube nested real-time PCR for the detection of walnut allergens in sponge cakes

2013 ◽  
Vol 54 (2) ◽  
pp. 1722-1729 ◽  
Author(s):  
Joana Costa ◽  
Maria Beatriz P.P. Oliveira ◽  
Isabel Mafra
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Thermal Processing ◽  
The Novel ◽  
Single Tube

Development of a single-tube duplex EvaGreen real-time PCR for the detection and identification of EHV-1 and EHV-4

2014 ◽  
Vol 98 (9) ◽  
pp. 4179-4186 ◽  
Author(s):  
Zhe Hu ◽  
Chao Zhu ◽  
Hao Chang ◽  
Wei Guo ◽  
Diqiu Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Single Tube ◽  
Detection And Identification

Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

2021 ◽  
Author(s):  
Kundan Tandel ◽  
Mahadevan Kumar ◽  
G.S. Bhalla ◽  
S.P.S. Shergill ◽  
Vijaya Swarnim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Dengue Virus Serotypes

scholarly journals Validation of a real-time PCR assay for high-throughput detection of Avibacterium paragallinarum in chicken respiratory sites

2019 ◽  
Vol 31 (5) ◽  
pp. 714-718 ◽  
Author(s):  
Kristin A. Clothier ◽  
Simone Stoute ◽  
Andrea Torain ◽  
Beate Crossley
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Bacterial Flora ◽  
Clinical Samples ◽  
Single Tube ◽  
Avibacterium Paragallinarum ◽  
Normal Bacterial Flora ◽  
Close Relatives

Avibacterium paragallinarum is the causative agent of infectious coryza, a highly contagious respiratory disease in chickens. Given its fastidious nature, this bacterium is difficult to recover and identify, particularly from locations colonized by normal bacterial flora. Standard PCR methods have been utilized for detection but are labor-intensive and not feasible for high-throughput testing. We evaluated a real-time PCR (rtPCR) method targeting the HPG-2 region of A. paragallinarum, and validated a high-throughput extraction for this assay. Using single-tube extraction, the rtPCR detected 4 A. paragallinarum (ATCC 29545T and 3 clinical) isolates with a limit of detection (LOD) of 10 cfu/mL and a PCR efficiency of 89–111%. Cross-reaction was not detected with 33 non– A. paragallinarum, all close relatives from the family Pasteurellaceae. Real-time PCR testing on extracts of 66 clinical samples (choana, sinus, or trachea) yielded 98.2% (35 of 36 on positives, 30 of 30 on negatives) agreement with conventional PCR. Duplicate samples tested in a 96-well format extraction in parallel with the single-tube method produced equivalent LOD on all A. paragallinarum isolates, and 96.8% agreement on 93 additional clinical samples extracted with both procedures. This A. paragallinarum rtPCR can be utilized for outbreak investigations and routine monitoring of susceptible flocks.

Matrix-normalised real-time PCR approach to quantify soybean as a potential food allergen as affected by thermal processing

2017 ◽  
Vol 221 ◽  
pp. 1843-1850 ◽  
Author(s):  
Joana Costa ◽  
Joana S. Amaral ◽  
Liliana Grazina ◽  
M. Beatriz P.P. Oliveira ◽  
Isabel Mafra
Keyword(s):  
Real Time ◽  
Food Allergen ◽  
Real Time Pcr ◽  
Thermal Processing ◽  
Potential Food

Single-tube multiplex real-time PCR assay for rapid and reliable detection of HLA-A*31:01 allele

2018 ◽  
Vol 19 (10) ◽  
pp. 837-846 ◽  
Author(s):  
Tingting Zhang ◽  
Ying Xiao ◽  
Yanxia Wang ◽  
Yanwei Li ◽  
Lirong Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Reliable Detection

Novel approach based on single-tube nested real-time PCR to detect almond allergens in foods

2013 ◽  
Vol 51 (1) ◽  
pp. 228-235 ◽  
Author(s):  
Joana Costa ◽  
Maria Beatriz P.P. Oliveira ◽  
Isabel Mafra
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Single Tube ◽  
Novel Approach

scholarly journals Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material

2010 ◽  
Vol 16 (6) ◽  
pp. 704-710 ◽  
Author(s):  
A.M.C. Bergmans ◽  
M. van der Ent ◽  
A. Klaassen ◽  
N. Böhm ◽  
G.I. Andriesse ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Material ◽  
Single Tube ◽  
Detection And Identification ◽  
Dermatophyte Species
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