Efficiency evaluation of a DNA extraction and purification protocol on archival formalin-fixed and paraffin-embedded tissue

2010 ◽  
Vol 194 (1-3) ◽  
pp. e25-e28 ◽  
Author(s):  
A. Farrugia ◽  
C. Keyser ◽  
B. Ludes
Keyword(s):  
Dna Extraction ◽  
Efficiency Evaluation ◽  
Extraction And Purification ◽  
Purification Protocol ◽  
Formalin Fixed

Optimization of genomic DNA extraction from formalin-fixed fish specimens

2013 ◽  
Vol 19 (6) ◽  
pp. 1068-1073
Author(s):  
Xiaolan KONG ◽  
Zuozhi CHEN ◽  
Lin LIN ◽  
Chunhou LI ◽  
Peiwen LIANG
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
Formalin Fixed

Facile and rapid DNA extraction and purification from food matrices using IFAST (immiscible filtration assisted by surface tension)

The Analyst ◽  
2012 ◽  
Vol 137 (17) ◽  
pp. 4023 ◽  
Author(s):  
Lindsay N. Strotman ◽  
Guangyun Lin ◽  
Scott M. Berry ◽  
Eric A. Johnson ◽  
David J. Beebe
Keyword(s):  
Surface Tension ◽  
Dna Extraction ◽  
Extraction And Purification ◽  
Food Matrices

A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure

2003 ◽  
Vol 54 (2) ◽  
pp. 165-175 ◽  
Author(s):  
Teegan Trochimchuk ◽  
John Fotheringham ◽  
Edward Topp ◽  
Heidi Schraft ◽  
Kam Tin Leung
Keyword(s):  
Escherichia Coli ◽  
Dna Extraction ◽  
Cattle Manure ◽  
Escherichia Coli O157 ◽  
Extraction And Purification ◽  
Purification Methods

scholarly journals DNA extraction from fresh-frozen and formalin-fixed, paraffinembedded human brain tissue

2013 ◽  
Vol 29 (5) ◽  
pp. 649-654 ◽  
Author(s):  
Jian-Hua Wang ◽  
Amany Gouda-Vossos ◽  
Nicolas Dzamko ◽  
Glenda Halliday ◽  
Yue Huang
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Dna Extraction ◽  
Human Brain Tissue ◽  
Fresh Frozen ◽  
Formalin Fixed

scholarly journals Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis

2019 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Ezzouhra El Maaiden ◽  
Youssef El Kharrassi ◽  
Abdel Khalid Essamadi ◽  
Khadija Moustaid ◽  
Boubker Nasser
Keyword(s):  
Dna Extraction ◽  
Sodium Dodecyl ◽  
Model Organism ◽  
Tetrahymena Pyriformis ◽  
Pyrrolidine Dithiocarbamate ◽  
Restriction Enzyme Digestion ◽  
Chelex 100 ◽  
Extraction And Purification ◽  
Ammonium Pyrrolidine Dithiocarbamate ◽  
Triton X

Tetrahymena pyriformis (protozoa) is intensely investigated as a model organism, offering numerous advantages in comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is a vital step of any molecular experiment, here a new mixed surfactant (Sodium dodecyl sulfate (SDS) 20%/Triton X-100) was adopted for effective DNA extraction from Tetrahymena pyriformis under an easy, fast protocol. The efficiency of this technique was then compared with three widely-used alternative techniques, namely the Chelex 100 matrix, Ammonium pyrrolidine dithiocarbamate (APD) complex and SDS–chloroform methods. DNA extraction was analyzed by pulsed-field gel electrophoresis, spectral measurement, fluorometry (Qubit), restriction enzyme digestion, and polymerase chain reaction. Data analysis revealed that the quantity and quality of the recovered DNA varied depending on the applied DNA extraction method. The new method (SDS 20%/Triton X-100) was the most efficient for extracting DNA from Tetrahymena pyriformis with high integrity and purity, affordable cost, less time, and suitability for molecular applications.

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