Characterization of a 1-cysteine peroxiredoxin from big-belly seahorse ( Hippocampus abdominalis ); insights into host antioxidant defense, molecular profiling and its expressional response to septic conditions

In last years there is increasing interest in radiogenomics and the characterization of DNA array molecular profiles that can predict tumor and no tumor tissues radioresponse. Ongoing studies carried out worldwide in the banking of tumor and no tumor samples give evidence that perspective markers for response prediction in individual patient to intended radiation therapy can be some apoptotic indexes, spectrum a number of specific proteins, and DNA-based microarray molecular profiling analysis as well determination of single nucleotide polymorphisms in genome of the patients. So far there are only a few robust reports of molecular markers predicting tumor and no tumor tissues response to radiation. The results of new studies, which in future should be validated in larger definitive trials, are likely to see in nearest years. It is needed to determine technologies of methods and to define more precisely areas of its applications.

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Spatial molecular profiling: platforms, applications and analysis tools

Briefings in Bioinformatics ◽

10.1093/bib/bbaa145 ◽

2020 ◽

Author(s):

Minzhe Zhang ◽

Thomas Sheffield ◽

Xiaowei Zhan ◽

Qiwei Li ◽

Donghan M Yang ◽

...

Keyword(s):

Spatial Organization ◽

Spatial Information ◽

Molecular Profiling ◽

Tissue Imaging ◽

Imaging Data ◽

Tissue Heterogeneity ◽

Tissue Dissociation ◽

Recent Developments ◽

Spatial Locations

Abstract Molecular profiling technologies, such as genome sequencing and proteomics, have transformed biomedical research, but most such technologies require tissue dissociation, which leads to loss of tissue morphology and spatial information. Recent developments in spatial molecular profiling technologies have enabled the comprehensive molecular characterization of cells while keeping their spatial and morphological contexts intact. Molecular profiling data generate deep characterizations of the genetic, transcriptional and proteomic events of cells, while tissue images capture the spatial locations, organizations and interactions of the cells together with their morphology features. These data, together with cell and tissue imaging data, provide unprecedented opportunities to study tissue heterogeneity and cell spatial organization. This review aims to provide an overview of these recent developments in spatial molecular profiling technologies and the corresponding computational methods developed for analyzing such data.

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Molecular Profiling: A Case ofZBTB16-RARAAcute Promyelocytic Leukemia

Case Reports in Hematology ◽

10.1155/2017/7657393 ◽

2017 ◽

Vol 2017 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Stephen E. Langabeer ◽

Lisa Preston ◽

Johanna Kelly ◽

Matt Goodyer ◽

Ezzat Elhassadi ◽

...

Keyword(s):

Retinoic Acid ◽

Clinical Course ◽

Molecular Profiling ◽

Promyelocytic Leukemia ◽

Fusion Product ◽

Rare Form ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid ◽

Generation Sequencing

Several variantRARAtranslocations have been reported in acute promyelocytic leukemia (APL) of which the t(11;17)(q23;q21), which results in aZBTB16-RARAfusion, is the most widely identified and is largely resistant to therapy with all-trans retinoic acid (ATRA). The clinical course together with the cytogenetic and molecular characterization of a case of ATRA-unresponsiveZBTB16-RARAAPL is described. Additional mutations potentially cooperating with the translocation fusion product in leukemogenesis have been hitherto unreported inZBTB16-RARAAPL and were sought by application of a next-generation sequencing approach to detect those recurrently found in myeloid malignancies. This technique identified a solitary, low level mutation in theCEBPAgene. Molecular profiling of additional mutations may provide a platform to individualise therapeutic management in patients with this rare form of APL.

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