Changes in Trichoderma asperellum enzyme expression during parasitism of the cotton root rot pathogen Phymatotrichopsis omnivora

2015 ◽  
Vol 119 (4) ◽  
pp. 264-273 ◽  
Author(s):  
Cesar Guigón-López ◽  
Francisco Vargas-Albores ◽  
Víctor Guerrero-Prieto ◽  
Michelina Ruocco ◽  
Matteo Lorito
Plant Disease ◽  
2014 ◽  
Vol 98 (9) ◽  
pp. 1205-1212 ◽  
Author(s):  
M. Arif ◽  
S. Dobhal ◽  
P. A. Garrido ◽  
G. K. Orquera ◽  
A. S. Espíndola ◽  
...  

Phymatotrichopsis omnivora, the causal pathogen of cotton root rot, is a devastating ascomycete that affects numerous important dicotyledonous plants grown in the southwestern United States and northern Mexico. P. omnivora is notoriously difficult to isolate from infected plants; therefore methods for accurate and sensitive detection directly from symptomatic and asymptomatic plant samples are needed for disease diagnostics and pathogen identification. Primers were designed for P. omnivora based on consensus sequences of the nuclear ribosomal internal transcribed spacer (ITS) region of geographically representative isolates. Primers were compared against published P. omnivora sequences and validated against DNA from P. omnivora isolates and infected plant samples. The primer combinations amplified products from a range of P. omnivora isolates representative of known ITS haplotypes using standard end-point polymerase chain reaction (PCR) methodology. The assays detected P. omnivora from infected root samples of cotton (Gossypium hirsutum) and alfalfa (Medicago sativa). Healthy plants and other relevant root pathogens did not produce PCR products with the P. omnivora–specific primers. Primer pair PO2F/PO2R was the most sensitive in end-point PCR assays and is recommended for use for pathogen identification from mycelial tissue and infected plant materials when quantitative PCR (qPCR) is not available. Primer pair PO3F/PO2R was highly sensitive (1 fg) when used in SYBR Green qPCR assays and is recommended for screening of plant materials potentially infected by P. omnivora or samples with suboptimal DNA quality. The described PCR-based detection methods will be useful for rapid and sensitive screening of infected plants in diagnostic laboratories, plant health inspections, and plant breeding programs.


2020 ◽  
Vol 12 (15) ◽  
pp. 2453
Author(s):  
Tianyi Wang ◽  
J. Alex Thomasson ◽  
Thomas Isakeit ◽  
Chenghai Yang ◽  
Robert L. Nichols

Cotton root rot (CRR), caused by the fungus Phymatotrichopsis omnivora, is a destructive cotton disease that mainly affects the crop in Texas. Flutriafol fungicide applied at or soon after planting has been proven effective at protecting cotton plants from being infected by CRR. Previous research has indicated that CRR will reoccur in the same regions of a field as in past years. CRR-infected plants can be detected with aerial remote sensing (RS). As unmanned aerial vehicles (UAVs) have been introduced into agricultural RS, the spatial resolution of farm images has increased significantly, making plant-by-plant (PBP) CRR classification possible. An unsupervised classification algorithm, PBP, based on the Superpixel concept, was developed to delineate CRR-infested areas at roughly the single-plant level. Five-band multispectral data were collected with a UAV to test these methods. The results indicated that the single-plant level classification achieved overall accuracy as high as 95.94%. Compared to regional classifications, PBP classification performed better in overall accuracy, kappa coefficient, errors of commission, and errors of omission. The single-plant fungicide application was also effective in preventing CRR.


2011 ◽  
Vol 49 ◽  
pp. 68-75 ◽  
Author(s):  
P. Hu ◽  
A.S. Wang ◽  
A.S. Engledow ◽  
E.B. Hollister ◽  
K.L. Rothlisberger ◽  
...  

Science ◽  
1940 ◽  
Vol 92 (2397) ◽  
pp. 533-534
Author(s):  
W. N. EZEKIEL
Keyword(s):  
Root Rot ◽  

Science ◽  
1940 ◽  
Vol 92 (2397) ◽  
pp. 533-534
Author(s):  
Walter N. Ezekiel
Keyword(s):  
Root Rot ◽  

Science ◽  
1940 ◽  
Vol 92 (2397) ◽  
pp. 533-534
Author(s):  
Walter N. Ezekiel
Keyword(s):  
Root Rot ◽  

2011 ◽  
Author(s):  
Chenghai Yang ◽  
Gary N Odvody ◽  
Carlos J Fernandez ◽  
Juan A Landivar ◽  
Robert L Nichols

2016 ◽  
Vol 19 (s1) ◽  
pp. 15-17
Author(s):  
Jacek Nawrocki ◽  
Anna Pogodzińska

Abstract In two-year field experiments (2014 and 2015), the effect of used preparations on health status of leaves and roots and bulbs on two cultivars of garlic: ‘Arkus’ and ‘Garpek’ was studied. During investigations: Polyversum WP (Pythium oligandrum), Trifender WP (Trichoderma asperellum) and RhizoVital 42 (Bacillus amyloliquefaciens) and standard fungicide Topsin M 500 SC (tiophanate methyl) were used. Unprotected plants presented control. The obtained results showed that in the first year of the studies, all the tested formulations effectively protected the roots and bulbs of garlic against rot, except RhizoVital 42 for ‘Arkus’ variety and Trifender WP for cultivar ‘Garpek’. In 2015, all tested preparations, without exception, limited root rot and the rot of basal part of bulbs both cultivars of garlic. The applied biological preparations had no significant effect on health of the leaves of garlic in 2014 and in 2015 for cultivar ‘Garpek’, while in the second year of studies, all the tested formulations effectively limited the dieback of leaves of the garlic cultivar ‘Arkus’.


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