Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion

2020 ◽  
Vol 124 (5) ◽  
pp. 297-303 ◽  
Author(s):  
Gabriela Braga Rodrigues ◽  
Guilherme Thomaz Pereira Brancini ◽  
Marcelo Rodrigues Pinto ◽  
Fernando Lucas Primo ◽  
Mark Wainwright ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Photodynamic Inactivation ◽  
Aluminum Phthalocyanine

scholarly journals Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and ReducesIn VivoPathogenicity

2012 ◽  
Vol 57 (1) ◽  
pp. 445-451 ◽  
Author(s):  
Ilka Tiemy Kato ◽  
Renato Araujo Prates ◽  
Caetano Padial Sabino ◽  
Beth Burgwyn Fuchs ◽  
George P. Tegos ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Virulence Factors ◽  
Sodium Dodecyl ◽  
Systemic Infection ◽  
Tube Formation ◽  
Photodynamic Inactivation ◽  
Content Type ◽  
Daughter Cells

ABSTRACTThe objective of this study was to evaluate whetherCandida albicansexhibits altered pathogenicity characteristics following sublethal antimicrobial photodynamic inactivation (APDI) and if such alterations are maintained in the daughter cells.C. albicanswas exposed to sublethal APDI by using methylene blue (MB) as a photosensitizer (0.05 mM) combined with a GaAlAs diode laser (λ 660 nm, 75 mW/cm2, 9 to 27 J/cm2).In vitro, we evaluated APDI effects onC. albicansgrowth, germ tube formation, sensitivity to oxidative and osmotic stress, cell wall integrity, and fluconazole susceptibility.In vivo, we evaluatedC. albicanspathogenicity with a mouse model of systemic infection. Animal survival was evaluated daily. Sublethal MB-mediated APDI reduced the growth rate and the ability ofC. albicansto form germ tubes compared to untreated cells (P< 0.05). Survival of mice systemically infected withC. albicanspretreated with APDI was significantly increased compared to mice infected with untreated yeast (P< 0.05). APDI increasedC. albicanssensitivity to sodium dodecyl sulfate, caffeine, and hydrogen peroxide. The MIC for fluconazole forC. albicanswas also reduced following sublethal MB-mediated APDI. However, none of those pathogenic parameters was altered in daughter cells ofC. albicanssubmitted to APDI. These data suggest that APDI may inhibit virulence factors and reducein vivopathogenicity ofC. albicans. The absence of alterations in daughter cells indicates that APDI effects are transitory. The MIC reduction for fluconazole following APDI suggests that this antifungal could be combined with APDI to treatC. albicansinfections.

Pathogenicity of Candida tropicalis and Candida albicans after gastrointestinal inoculation in mice

1980 ◽  
Vol 29 (2) ◽  
pp. 808-813 ◽  
Author(s):  
J R Wingard ◽  
J D Dick ◽  
W G Merz ◽  
G R Sandford ◽  
R Saral ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Mouse Model ◽  
Lung Tissue ◽  
Candida Tropicalis ◽  
Cytotoxic Drugs ◽  
Clinical Isolates ◽  
Yeast Cells ◽  
Gastrointestinal Mucosa ◽  
Compromised Host ◽  
Adult Mice

The ability of clinical isolates of Candida albicans and candida tropicalis to invade through normal and damaged gastrointestinal mucosa was determined. Adult mice were treated with either gentamicin or gentamicin and cytarabine. Suspensions of yeast cells (10(7)) were administered through a catheter intraesophageally. Invasion was determined by culturing liver, kidney, and lung tissue from mice sacrificed after 48 h. C. albicans and C. tropicalis were incapable of invading through normal gastrointestinal mucosa in mice treated only with gentamicin. Two isolates of C. tropicalis penetrated the damaged gastrointestinal mucosa in 69% (49 of 71) of mice treated with gentamicin and cytarabine. In contrast, three isolates of C. albicans penetrated he damaged gastrointestinal mucosa in only 23% (14 of 62) of mice. These results suggest that C. tropicalis is more capable of invading through damaged gastrointestinal mucosa than C. albicans. The observations in this mouse model parallel those seen in patients on cytotoxic drugs. Therefore, this model offers a tool for investigation of the pathogenicity of these organisms in a model analogous to the compromised host.

scholarly journals Interactions between Terpinen-4-ol and Nystatin on biofilm of Candida albicans and Candida tropicalis

2018 ◽  
Vol 29 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Caroline Coradi Tonon ◽  
Renata Serignoli Francisconi ◽  
Ester Alves Ferreira Bordini ◽  
Patrícia Milagros Maquera Huacho ◽  
Janaína de Cássia Orlandi Sardi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Enzymatic Activity ◽  
Candida Tropicalis ◽  
Microtiter Plate ◽  
Synergistic Activity ◽  
Oral Keratinocytes ◽  
Mixed Species ◽  
Colony Forming Units ◽  
Anti Fungal ◽  
Oral Cells

Abstract The aim of this study was to evaluate the antifungal activity of Terpinen-4-ol associated with nystatin, on single and mixed species biofilms formed by Candida albicans and Candida tropicalis, as well as the effect of terpinen-4-ol on adhesion in oral cells and the enzymatic activity. The minimum inhibitory concentrations and minimum fungicide concentrations of terpinen-4-ol and nystatin on Candida albicans and Candida tropicalis were determined using the microdilution broth method, along with their synergistic activity (“checkerboard” method). Single and mixed species biofilms were prepared using the static microtiter plate model and quantified by colony forming units (CFU/mL). The effect of Terpinen-4-ol in adhesion of Candida albicans and Candida tropicalis in coculture with oral keratinocytes (NOK Si) was evaluated, as well as the enzymatic activity by measuring the size of the precipitation zone, after the growth agar to phospholipase, protease and hemolysin. Terpinen-4-ol (4.53 mg mL-1) and nystatin (0.008 mg mL-1) were able to inhibit biofilms growth, and a synergistic antifungal effect was showed with the drug association, reducing the inhibitory concentration of nystatin up to 8 times in single biofilm of Candida albicans, and 2 times in mixed species biofilm. A small decrease in the adhesion of Candida tropicalis in NOK Si cells was showed after treatment with terpinen-4-ol, and nystatin had a greater effect for both species. For enzymatic activity, the drugs showed no action. The effect potentiated by the combination of terpinen-4-ol and nystatin and the reduction of adhesion provide evidence of its potential as an anti-fungal agent.

Candida tropicalis meningitis: a case report

1981 ◽  
Vol 95 (11) ◽  
pp. 1149-1151 ◽  
Author(s):  
B. Chattopadhyay
Keyword(s):  
Candida Albicans ◽  
Case Report ◽  
Adult Patient ◽  
Middle Ear ◽  
Candida Tropicalis ◽  
Antifungal Agent ◽  
Healthy Adult ◽  
Uneventful Recovery ◽  
Healthy Patient ◽  
Middle Ear Disease

SummaryA 54-year-old man presented with candida tropicalis meningitis after exploration of the mastoid. He responded well to the combined intravenous administration of amphotericin B and 5 fluorocytosine. But 5 fluorocytosine had to be withdrawn when the candida proved to be resistant to this antifungal agent. However, the patient made an uneventful recovery. This seems to be the first report of a candida tropicalis meningitis in an otherwise healthy adult patient in this country.Infection of the meninges with candida remains rare (Black, 1970). Most of them are caused by candida albicans in patients with some other underlying conditions or those on prolonged antibiotic, corticosteroid, cytotoxic, immunosuppresive therapies. The purpose of this brief communication is to draw attention to its occurrence in a healthy patient following exploration of the mastoid for chronic suppurative middle-ear disease.

scholarly journals Comparative Study on the Efficiency of the Photodynamic Inactivation of Candida albicans Using CdTe Quantum Dots, Zn(II) Porphyrin and Their Conjugates as Photosensitizers

Molecules ◽  
2015 ◽  
Vol 20 (5) ◽  
pp. 8893-8912 ◽  
Author(s):  
Osnir Viana ◽  
Martha Ribeiro ◽  
Andréa Rodas ◽  
Júlio Rebouças ◽  
Adriana Fontes ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Candida Albicans ◽  
Comparative Study ◽  
Cdte Quantum Dots ◽  
Photodynamic Inactivation

FORMACIÓN DE BIOPELÍCULAS Y SENSIBILIDAD A ANTIFÚNGICOS DE Candida albicans, Candida tropicalis Y Candida glabrata

2021 ◽  
pp. 93-105
Author(s):  
Alejandra Paula Espinosa Texis ◽  
Débora Vázquez Domínguez ◽  
David Iván Loaiza Toscuento ◽  
Teresita Spezzia Mazzocco
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata

scholarly journals Synergistic Antifungal Activity of Chitosan with Fluconazole against Candida albicans, Candida tropicalis, and Fluconazole-Resistant Strains

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5114
Author(s):  
Wei-Hsuan Lo ◽  
Fu-Sheng Deng ◽  
Chih-Jung Chang ◽  
Ching-Hsuan Lin
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Tropicalis ◽  
Inhibitory Effect ◽  
Antifungal Drugs ◽  
Drug Resistant ◽  
Combinational Treatment ◽  
Fluconazole Resistant ◽  
Resistant Strains ◽  
Drug Resistant Strains

(1) Background: Few antifungal drugs are currently available, and drug-resistant strains have rapidly emerged. Thus, the aim of this study is to evaluate the effectiveness of the antifungal activity from a combinational treatment of chitosan with a clinical antifungal drug on Candida albicans and Candida tropicalis. (2) Methods: Minimum inhibitory concentration (MIC) tests, checkerboard assays, and disc assays were employed to determine the inhibitory effect of chitosan with or without other antifungal drugs on C. albicans and C. tropicalis. (3) Results: Treatment with chitosan in combination with fluconazole showed a great synergistic fungicidal effect against C. albicans and C. tropicalis, but an indifferent effect on antifungal activity when challenged with chitosan-amphotericin B or chitosan-caspofungin simultaneously. Furthermore, the combination of chitosan and fluconazole was effective against drug-resistant strains. (4) Conclusions: These findings provide strong evidence that chitosan in combination with fluconazole is a promising therapy against two Candida species and its drug-resistant strains.

scholarly journals Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

2009 ◽  
Vol 42 (2) ◽  
pp. 188-191 ◽  
Author(s):  
Danielle Patrícia Cerqueira Macêdo ◽  
Aline Mary de Almeida Farias ◽  
Reginaldo Gonçalves de Lima Neto ◽  
Vanessa Karina Alves da Silva ◽  
André Ferraz Goiana Leal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei

Infecções por leveduras são freqüentes em imunocomprometidos, contudo espécies emergentes têm alterado o perfil epidemiológico. A habilidade de secretar proteases tem sido associada à patogenicidade do gênero Candida. Esta pesquisa teve como objetivos diagnosticar leveduroses em pacientes imunocomprometidos e avaliar a virulência dos agentes etiológicos baseado em teste de secreção de protease utilizando soro de albumina bovina como substrato. Do total de 104 pacientes estudados, 19 apresentaram episódios de leveduroses. O trato respiratório (63,2%), seguido pelo trato urinário (10,5%) foram os locais mais comuns de infecção. Candida albicans, Candida parapsilosis, Candida tropicalis e espécies emergentes como Candida krusei e Candida guilliermondii foram isoladas. Cinco isolados de Candida parapsilosis e um de Candida albicans e Candida guilliermondii exibiram alta atividade enzimática. Concluímos que a caracterização enzimática de isolados de Candida pode ser um útil marcador prognóstico, especialmente em imunocomprometidos, uma vez que leveduroses nestes pacientes são geralmente graves.

Photodynamic inactivation of Candida albicans mediated by a low density of light energy

2013 ◽  
Author(s):  
Gabriel Salles Barbério ◽  
Soraia Veloso da Costa ◽  
Mariana dos Santos Silva ◽  
Thaís Marchini de Oliveira ◽  
Thiago Cruvinel Silva ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Light Energy ◽  
Low Density ◽  
Photodynamic Inactivation
Sign in / Sign up

Export Citation Format

Share Document