scholarly journals A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions

2020 ◽  
Author(s):  
Lucas Gelain ◽  
José Geraldo da Cruz Pradella ◽  
Aline Carvalho da Costa ◽  
Luuk van der Wielen ◽  
Walter M. van Gulik
Keyword(s):  
Trichoderma Harzianum ◽  
Extracellular Polysaccharides ◽  
Intracellular Metabolites

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

2020 ◽  
Author(s):  
Ian Sims ◽  
A Bacic
Keyword(s):  
Uronic Acid ◽  
Cell Suspension Cultures ◽  
Anion Exchange Chromatography ◽  
Nicotiana Plumbaginifolia ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Selective Precipitation ◽  
The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

FERMENTASI JERAMI JAGUNG MENGGUNAKAN KAPANG TRICHODERMA HARZIANUM DITINJAU DARI KARAKTERISTIK DEGRADASI

2017 ◽  
Vol 1 (1) ◽  
pp. 43-49
Author(s):  
Suryadi Suryadi ◽  
Darlis Darlis ◽  
Suhessy Syarif ◽  
M. Afdal
Keyword(s):  
Trichoderma Harzianum ◽  
In Sacco

Penelitian ini bertujuan untuk mengetahui lama waktu fermentasi dan karakteristik degradasi komponen serat jerami jagung fermentasi secara In sacco. Fermentasi jerami jagung secara padat menggunakan  Trichoderma harzianum sebagai stater. Sebanyak 2,5 gram urea, 2,5 gram molases, 2,5 ml sediaan  Trichoderma harzianum dicampur dengan air menjadi 20 ml yang kemudian disemprotkan pada 1000 gram jerami jagung segar. Selanjutnya jerami jagung dimasukkan ke dalam toples plastik dan diperam sesuai dengan perlakuan yaitu 4, 8, 12 dan 16 hari. Uji karakteristik degradasi jerami jagung fermentasi dilakukan dengan metode In sacco atau nylon bag technique. Sebanyak 6 gram sampel jerami dimasukkan ke dalam kantong nylon dengan ukuran 140, 80 mm diinkubasi ke dalam rumen sapi dengan interval waktu 6, 12, 24, 48 dan 72. Penelitian ini  menggunakan rancangan acak lengkap (RAL) dengan perlakuan 4 lama fermentasi dan ulangan 3 untuk tiap perlakuan. Peubah yang diukur adalah karakteristik degradasi meliputi : Nilai fraksi a, nilai fraksi b dan nilai fraksi c dari NDF, ADF dan Hemiselulosa  Jerami jagung fermentasi. Hasil penelitian ini menunjukkan bahwa lama fermentasi berpengaruh nyata terhadap nilai fraksi (a) dan nilai fraksi (b) dari NDF, nilai fraksi (c) dari ADF dan Hemiselulosa, tetapi tidak berpengaruh nyata pada fraksi (a) dan fraksi (b) dari ADF,  fraksi (a) dan fraksi (b) dari hemiselulosa, fraksi (c) dari NDF jerami jagung fermentasi. Kesimpulan: Fermentasi jerami jagung dengan Trichoderma harzianum dapat meningkatkan nilai fraksi (a) dari NDF, ADF dan laju degradasi NDF ADF dan Hemiselulosa. Lama fermentasi yang terbaik pada jerami jagung fermentasi diperoleh pada perlakuan 16 hari.

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