A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics

Gene ◽  
2007 ◽  
Vol 395 (1-2) ◽  
pp. 116-124 ◽  
Author(s):  
Hiroshi Honda ◽  
Kenji Ichiyanagi ◽  
Jun Suzuki ◽  
Takao Ono ◽  
Hideki Koyama ◽  
...  
Keyword(s):  
Cell Line ◽  
Reverse Genetics ◽  
Dt40 Cell ◽  
Chicken Dt40 Cell ◽  
New System

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Chicken DT40 Cell Line

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 372
Author(s):  
Aleksandra Dunislawska ◽  
Anna Slawinska ◽  
Maria Siwek
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Reference Gene ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Relative Gene Expression ◽  
Dt40 Cell ◽  
Relative Gene ◽  
Chicken Dt40 Cell ◽  
Selection Of

The selection of a suitable reference gene assures a reliable gene expression analysis when using the qPCR method. Normalization of the reaction is based on the basic metabolism genes. These genes show a constant, unregulated expression in all cells and function throughout their lifetime. In the current study, seven reference gene candidates were screened using RT-qPCR, to determine the best-matched pair of reference genes in the chicken DT40 cell line. The DT40 was derived from bursal lymphoma cells that were subjected to RAV-1 bird retroviral infection. It is a simplified in vitro model that allows tracking the direct interaction of stimulants on the lymphoid population and profiling of the hepatocellular B cell transcriptome. The reference gene analysis was carried out using statistical tools integrating four independent methods—geNorm, Best Keeper, NormFinder, delta Ct and RefFinder. Based on the selected reference genes, the relative gene expression analysis was done using the ddCt method. Complete relative gene expression study on a panel of the target genes revealed that proper selection of reference genes depending on the tissue eliminate decreases in data quality. The SDHA and RPL4 genes constitute stable internal controls as reference genes when analyzing gene expression in the DT40 cell line.

scholarly journals Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction

2013 ◽  
Vol 77 (4) ◽  
pp. 228-233 ◽  
Author(s):  
Eiko N. Minakawa ◽  
Hodaka Yamakado ◽  
Atsushi Tanaka ◽  
Kengo Uemura ◽  
Shunichi Takeda ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Mitochondrial Dysfunction ◽  
Cell Line ◽  
Dt40 Cell ◽  
Familial Parkinson’S Disease ◽  
Chicken Dt40 Cell

DNA Repair Studies: Experimental Evidence in Support of Chicken DT40 Cell Line as a Unique Model

2001 ◽  
Vol 20 (4) ◽  
pp. 11 ◽  
Author(s):  
Pawan Kumar Dhar ◽  
Eiichiro Sonoda ◽  
Akira Fujimori ◽  
Yukiko M. Yamashita ◽  
Shunichi Takeda
Keyword(s):  
Dna Repair ◽  
Experimental Evidence ◽  
Cell Line ◽  
Dt40 Cell ◽  
Unique Model ◽  
Chicken Dt40 Cell

scholarly journals The role of HERC2 and RNF8 ubiquitin E3 ligases in the promotion of translesion DNA synthesis in the chicken DT40 cell line

DNA Repair ◽  
2016 ◽  
Vol 40 ◽  
pp. 67-76 ◽  
Author(s):  
Mohiuddin ◽  
Shunsuke Kobayashi ◽  
Islam Shamima Keka ◽  
Guillaume Guilbaud ◽  
Julian Sale ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Cell Line ◽  
E3 Ligases ◽  
Translesion Dna Synthesis ◽  
Dt40 Cell ◽  
Translesion Dna ◽  
Chicken Dt40 Cell

scholarly journals Reduced X-Ray Resistance and Homologous Recombination Frequencies in a RAD54−/− Mutant of the Chicken DT40 Cell Line

Cell ◽  
1997 ◽  
Vol 89 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Olga Bezzubova ◽  
Arkadiy Silbergleit ◽  
Yuko Yamaguchi-Iwai ◽  
Shunichi Takeda ◽  
Jean-Marie Buerstedde
Keyword(s):  
Homologous Recombination ◽  
Cell Line ◽  
X Ray ◽  
Dt40 Cell ◽  
Chicken Dt40 Cell

scholarly journals Reverse genetic studies of homologous DNA recombination using the chicken B–lymphocyte line, DT40

2001 ◽  
Vol 356 (1405) ◽  
pp. 111-117 ◽  
Author(s):  
Eiichiro Sonoda ◽  
Ciaran Morrison ◽  
Yukiko M. Yamashita ◽  
Minoru Takata ◽  
Shunichi Takeda
Keyword(s):  
Cell Line ◽  
Reverse Genetics ◽  
B Lymphocyte ◽  
Dna Recombination ◽  
Temperature Sensitive ◽  
Immunoglobulin Gene ◽  
Genetic Studies ◽  
Transformed Cell Line ◽  
Variable Segment ◽  
Dt40 Cells

DT40 is an avian leucosis virus–transformed chicken B–lymphocyte line which exhibits high ratios of targeted to random integration of transfected DNA constructs. This efficient targeted integration may be related to the ongoing diversification of the variable segment of the immunoglobulin gene through homologous DNA recombination–controlled gene conversion. DT40s are a convenient model system for making gene–targeted mutants. Another advantage is the relative tractability of these cells, which makes it possible to disrupt multiple genes in a single cell and to generate conditionally gene–targeted mutants including temperature–sensitive mutants. There are strong phenotypic similarities between murine and DT40 mutants of various genes involved in DNA recombination. These similarities confirm that the DT40 cell line is a reasonable model for the analysis of vertebrate DNA recombination, despite obvious concerns associated with the use of a transformed cell line, which may have certain cell–line–specific characteristics. Here we describe our studies of homologous DNA recombination in vertebrate somatic cells using reverse genetics in DT40 cells.

Establishment of a New System for Determination of Coreceptor Usages of HIV Based on the Human Glioma NP-2 Cell Line

1999 ◽  
Vol 258 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Yasushi Soda ◽  
Nobuaki Shimizu ◽  
Atsushi Jinno ◽  
Hui-Yu Liu ◽  
Katsuaki Kanbe ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Glioma ◽  
New System
Sign in / Sign up

Export Citation Format

Share Document