Homotypic clustering of OsMYB4 binding site motifs in promoters of the rice genome and cellular-level implications on sheath blight disease resistance

Sheath blight disease caused by Rhizoctonia solani is the most destructive diseases in rice cultivation. Development of transgenic indica rice MR219 through Agrobacterium tumefaciens strain EHA 105 harbouring the plasmid pCAMBIA 1305.2 with endo-beta-1,3-1,4-glucanase gene from Bacillus SP 289 is one of the strategies to engineer disease resistance. Four optimisation parameters were examined such as Agrobacterium culture cell density (0.1 to 1.0 based on OD600nm), callus immersion time in the Agrobacterium culture (30 to 120 minutes), duration of the subsequent drying time (15 to 120 minutes) and co-cultivation period (1 to 6 days). Hygromycin-resistant embryogenic calli developed after 8 to 10 weeks of transformation. Improved transformation rates were achieved when calli were incubated with an Agrobacterium suspension with a culture density of OD 600nm 0.2 for 30 mins, followed by 90 mins of drying time and co-cultivation for 3 days. PCR analysis of the transformants confirmed the presence of Bacillus SP 289 endo-beta-1,3-1,4-glucanase and hpt genes in the rice genome. The transgenic rice plants obtained in this study will be tested against sheath blight disease.

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Mapping quantitative trait loci for sheath blight disease resistance in Yangdao 4 rice

Genetics and Molecular Research ◽

10.4238/2015.march.6.10 ◽

2015 ◽

Vol 14 (1) ◽

pp. 1636-1649 ◽

Cited By ~ 14

Author(s):

Z.H. Wen ◽

Y.X. Zeng ◽

Z.J. Ji ◽

C.D. Yang

Keyword(s):

Disease Resistance ◽

Quantitative Trait Loci ◽

Quantitative Trait ◽

Sheath Blight ◽

Sheath Blight Disease ◽

Trait Loci ◽

Blight Disease

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Loss of premature stop codon in theWall-Associated Kinase 91(OsWAK91) gene confers sheath blight disease resistance in rice

10.1101/625509 ◽

2019 ◽

Cited By ~ 3

Author(s):

Noor Al-Bader ◽

Austin Meier ◽

Matthew Geniza ◽

Yamid Sanabria Gongora ◽

James Oard ◽

...

Keyword(s):

Stop Codon ◽

Rice Genome ◽

Premature Stop Codon ◽

Genome Project ◽

Sheath Blight ◽

Chromosome 9 ◽

World Population ◽

Sheath Blight Disease ◽

Susceptible Allele ◽

Blight Disease

AbstractThe genetic arms race between pathogen and host plant is a tug of war that has been ongoing for millennia. The “battles” are those of disruption, restoration of signaling and information transmission on a subcellular level. One such battle occurs between rice an important crop that feeds 50% of the world population and the sheath blight disease (SB) caused by the fungusRhizoctonia solani. It results in 10□30% global yield loss annually and can reach 50% under severe outbreak. Many Receptor□like kinases (RLKs) are recruited as soldiers in these battles. Wall Associated Receptor Kinases (WAKs) a subfamily of receptor-like kinases have been shown to play a role in fungal defense. Here we show that rice geneOsWAK91, present in the major SB resistance QTL region on Chromosome□9 is a key component in defense against rice sheath blight. An SNP mutation C/T separates susceptible variety, Cocodrie (CCDR) from the resistant line MCR010277 (MCR). The resistant allele C results in the stop codon loss that results in 68 amino acids longer C□terminus carrying longer protein kinase domain and phosphorylation sites. Our genotype and phenotype analysis of the top 20 individuals of the double haploid SB population shows a strong correlation with the SNP. The susceptible allele appears as a recent introduction found in the japonica subspecies reference genome and a majority of the tropical and temperate japonica lines sequenced by the 3000 rice genome project. Multiple US commercial varieties with japonica background carry the susceptible allele and are known for SB susceptibility. This discovery opens the possibility of introducing resistance alleles into high yielding commercial varieties to reduce yield losses incurred by the disease.

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Overexpression ofJatropha curcasDefensin (JcDef) Enhances Sheath Blight Disease Resistance in Tobacco

Journal of Phytopathology ◽

10.1111/jph.12531 ◽

2016 ◽

Vol 165 (1) ◽

pp. 15-21 ◽

Cited By ~ 5

Author(s):

Qingwei Wang ◽

Shuhua Zhu ◽

Yao Liu ◽

Rui Li ◽

Shancai Tan ◽

...

Keyword(s):

Disease Resistance ◽

Sheath Blight ◽

Sheath Blight Disease ◽

Blight Disease

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Genome Editing of Rice PFT1 Gene to Study its Role in Rice Sheath Blight Disease Resistance

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2019.806.281 ◽

2019 ◽

Vol 8 (06) ◽

pp. 2356-2364

Author(s):

Priya Rajesh Shah ◽

S. Varanavasiappan ◽

E. Kokiladevi ◽

A. Ramanathan ◽

K.K. Kumar

Keyword(s):

Disease Resistance ◽

Genome Editing ◽

Sheath Blight ◽

Rice Sheath Blight ◽

Sheath Blight Disease ◽

Blight Disease

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RAVL1 Activates IDD3 to Negatively Regulate Rice Resistance to Sheath Blight Disease

Rice Science ◽

10.1016/j.rsci.2021.01.004 ◽

2021 ◽

Vol 28 (2) ◽

pp. 146-155

Author(s):

Sun Qian ◽

Yang Shuo ◽

Guo Xiaofan ◽

Wang Siting ◽

Jia Xintong ◽

...

Keyword(s):

Sheath Blight ◽

Sheath Blight Disease ◽

Blight Disease

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Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

EnviroScienteae ◽

10.20527/es.v16i1.8999 ◽

2020 ◽

Vol 16 (1) ◽

pp. 44

Author(s):

Hanisa Desy Ariani ◽

Noor Aidawati ◽

Dewi Arika Adriani

Keyword(s):

Pseudomonas Aeruginosa ◽

Rhizoctonia Solani ◽

Plant Protection ◽

Block Design ◽

Sheath Blight ◽

In Vitro Test ◽

Sheath Blight Disease ◽

Randomized Block Design ◽

Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

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BIOLOGICAL CONTROL OF BANDED LEAF AND SHEATH BLIGHT DISEASE (Rhizoctonia solani KUHN) IN CORN WITH FORMULATED Bacillus subtilis BR23

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v7n1.2006.1-7 ◽

2016 ◽

Vol 7 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Amran Muis ◽

Arcadio J. Quimio

Keyword(s):

Bacillus Subtilis ◽

Seed Germination ◽

Rhizoctonia Solani ◽

Seed Treatment ◽

The Philippines ◽

Sheath Blight ◽

Resistant Variety ◽

Sodium Hypochlorite Solution ◽

Sheath Blight Disease ◽

Blight Disease

Rhizoctonia solani Kuhn. causing banded leaf and sheath blight diseases is one of the important fungi of corn world wide. The fungus is commonly controlled by using fungicide because no resistant variety available. The objective of the study was to develop a seed treatment formulation of the selected Bacillus subtilis to control R. solani in corn. The study was conducted in the Department of Plant Pathology, College of Agriculture, University of the Philippines Los Bañòs, College, Laguna from May 2004 to August 2005, using sweet corn var. IPB Supersweet as test plant. Corn seeds were surface sterilized for 10 minutes in 1% sodium hypochlorite solution and 5% ethanol, washed thrice with sterile distilled water and air-dried. The seeds were coated with formulated B. subtilis BR23 and used for several experiments, such as evaluation for their germination and growth in the laboratory, effectively on R. solani in the baked and nonbaked field soil under greenhouse condition, and in the microplots artificially infested with R. solani. The treatment was compared with other standard seed treatment of synthetic fungicides such as captan (10 g per kg seeds) and metalaxyl (10 g per kg seeds). The experiments were designed in a completely random design with three replications. Parameters observed were seed germination, plant height, disease scores, and plant yield. Laboratory formulated B. subtilis BR23 used as seed treatment had no detrimental effects on seed germination and seedling vigor. In microplots artificially infested with a selected highly virulent R. solani, seed treatment with the same formulation increased grain yield by 27% compared to that of the control captan seed treatment with 14.4%. The studies showed the potential of B. subtilis BR23 for commercialization as a seed treatment for the control of banded leaf and sheath blight disease (R. solani) in corn.

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